Hypoxic preconditioning with cobalt ameliorates hypobaric hypoxia induced pulmonary edema in rat

Shukla, Dhananjay; Saxena, Sanjeev; Jayamurthy, P.; Shrivastava, Kalpana; Singh, Mrinalini; Shukla, Shirish; Malhotra, Vineet; Sairam, M.; Bansal, Anju

doi:10.1016/j.ejphar.2011.01.038

Cited by 38 publications

(28 citation statements)

References 44 publications

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“…Acute exposure of hypoxia leads to an increase in lung water content and total protein and albumin leakage in lavage fluids. The increased water content in lungs exposed to hypoxia appears to be due to leakage of fluids from intravascular to extravascular fluid compartments (8,20,21). Shukla et al (21) showed that bronchoalveolar fluid in lungs exposed to hypoxia for 5 h had a 1.5-fold increased VEGF level compared to the control group.…”

Section: Discussionmentioning

confidence: 99%

“…The increased water content in lungs exposed to hypoxia appears to be due to leakage of fluids from intravascular to extravascular fluid compartments (8,20,21). Shukla et al (21) showed that bronchoalveolar fluid in lungs exposed to hypoxia for 5 h had a 1.5-fold increased VEGF level compared to the control group. Many in vitro studies showed a clear link between hypoxia-induced VEGF and angiogenesis (22).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, damaged structural integrity of the alveolar/ capillary barrier increases pulmonary vascular leakage and causes edema in hypoxic pulmonary hypertension (17,21,27). During edema formation, the relation with hypoxia and MMPs has been examined both in vivo (10) and in vitro (11).…”

Section: Vegf (Average Of Staining Scores)mentioning

confidence: 99%

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The effects of hypoxia on the expression of vascular endothelialgrowth factor in broiler lung fibroblasts

Özyiğit

Kahraman²,

Akkoç³

2015

Turk J Vet Anim Sci

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IntroductionAscites (pulmonary hypertension), is a metabolic disorder that causes economic losses and is characterized by the accumulation of lymph fluid in the abdomen due to right heart failure in meat-type chickens. At necropsy, besides the lesions observed, other common findings such as generalized edema and expansion of the air capillaries are typically observed in the lung tissues of the chickens (1). Although environmental factors such as cold, high altitude, and diet play significant roles in the development of the syndrome (2), the main cause is hypoxia caused by increased oxygen demand, pulmonary hypertension, and extra workload on the heart (1-4).Vascular endothelial growth factor (VEGF), which is also known as edema factor, is a potent and key factor in the development of tumoral angiogenesis and edema formation by increasing vascular permeability (5,6). There are five VEGF products of alternative splicing, VEGF 121, VEGF 145, VEGF 165, VEGF 189, and VEGF 206 (7). VEGF 121 and VEGF 165 are secreted and both have mitogenic and permeability-inducing properties. VEGF has been synthesized in both animals and humans in endothelial cells, in mesenchymal cells, in alveolar macrophages of lung, in neurons, in renal epithelial cells, in connective tissue cells, and in tumor cells (8). Hypoxia has an important role in triggering VEGF (5,9). Investigations on people living at high altitudes showed that there might be a direct relationship between hypoxia and VEGF level due to the observed higher levels of serum VEGF, while in vitro studies on VEGF revealed that fibroblasts inflicted with mechanical damage and exposed to hypoxia showed an increase in VEGF levels compared with the control group (9).During the formation of pulmonary edema, matrix metalloproteinases (MMPs) and their relation with hypoxic stress were examined through both in vivo and in vitro studies for pulmonary hypertension syndrome (10,11). It was shown that MMPs have the potential for damaging the vascular basement membrane, but the mechanisms by which hypoxia causes ascites by increasing vascular permeability remains unclear. Thus, the aim of this study is to investigate the effects of hypoxic stress on VEGF, also known as edema factor, and its contribution to the pathogenesis of the disease in cultured lung fibroblasts of meat-type chickens. Materials and methods Cell cultureThis study was carried out after receiving written approval from the Scientific and Ethics Committee of Uludağ

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The effects of hypoxia on the expression of vascular endothelialgrowth factor in broiler lung fibroblasts

Özyiğit

Kahraman²,

Akkoç³

2015

Turk J Vet Anim Sci

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“…In support to these findings Chao et al [59] and Araneda and Tuesta [46] revealed that systemic inflammation of acute alveolar hypoxia observed in rats is not initiated by the low systemic tissue PO 2 , but rather a chemokine -monocyte chemoattractant Protein-1 (MCP-1) released by alveolar macrophages stimulated by hypoxia and transported by circulation therefore leads to microvascular inflammatory cascade. Exposure of rats to 9,142 m for 5 h [46] and 48 h at 7619 m [67] favors the generation of pulmonary edema and promotes the release of several inflammatory mediators (TNF-α, INF-γ, IL-1, MCP-1 and TGF-β) and adhesion molecules like ICAM-I and VACM-I in BALF. Rats exposed to 10% O 2 increased NFkB, mRNA of ICAM-1, VCAM-1, HIF1-α [66] with increased ROS by alveolar macrophages were described earlier [68].…”

Section: Discussionmentioning

confidence: 99%

Prophylactic Administration of Curcumin Abates the Incidence of Hypobaric Hypoxia Induced Pulmonary Edema in Rats: A Molecular Approach

Sagi¹

2014

J Pulm Respir Med

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“…Cobalt chloride (CoCl2) is a widely used hypoxia-mimetic; preconditioning with cobalt has been reported to exert protection against ischemic injury as well as decreasing pro-inflammatory cytokine levels ( Shukla et al 2011 ). Exposure to moderate hypoxia may lead to neurological phenomena such as paraesthesia, numbness or pain, possibly as a consequence of the modifications that appear in small DRG neurones.…”

Section: Introductionmentioning

confidence: 99%

Chronic cobalt exposure modifies nociception and orofacial inflammatory pain

et al. 2013

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Hypoxic preconditioning with cobalt ameliorates hypobaric hypoxia induced pulmonary edema in rat

Cited by 38 publications

References 44 publications

The effects of hypoxia on the expression of vascular endothelialgrowth factor in broiler lung fibroblasts

The effects of hypoxia on the expression of vascular endothelialgrowth factor in broiler lung fibroblasts

Prophylactic Administration of Curcumin Abates the Incidence of Hypobaric Hypoxia Induced Pulmonary Edema in Rats: A Molecular Approach

Chronic cobalt exposure modifies nociception and orofacial inflammatory pain

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