Hypoxic ADSCs-derived EVs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cells

Xue, Ke; Jiang, Yongkang; Zhang, Xiaodie; Wu, Jun; Qi, Lin; Li, Kai

doi:10.1080/21623945.2021.1945210

Cited by 13 publications

(14 citation statements)

References 59 publications

(65 reference statements)

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“…Four weeks after in vivo transplantation, samples were harvested and total RNA was extracted from every specimen. Followed previously described methods, cDNA was obtained by reverse transcription and gene expression was evaluated by real-time quantitative PCR analysis with the brilliant SYBR green qPCR kit (Strata gene, United States) ( Xue et al, 2019 ; Xue et al, 2021 ). SOX9, COL2, and aggrecan were employed to evaluate chondrogenic differentiation.…”

Section: Methodsmentioning

confidence: 99%

“…Due to its poor regenerative capacity, cartilage rarely produces effective regeneration, which causes patients pain and limitation in their usual activities of daily life ( Kilmer et al, 2020 ; Maihofer et al, 2021 ; Cui et al, 2023 ). Cell-assisted therapy has demonstrated promising results for cartilage regeneration ( Li et al, 2020 ; Xue et al, 2021 ; Gu et al, 2022 ). Stem cells have inspired research due to their special biological structure and characteristics at the molecular level ( Li et al, 2020 ; Rodriguez Ruiz et al, 2021 ; Palmquist-Gomes et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Comparative study of alginate and type I collagen as biomaterials for cartilage stem/progenitor cells to construct tissue-engineered cartilage in vivo

Zhang

Qi²,

Chen³

et al. 2023

Front. Bioeng. Biotechnol.

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With the help of biomaterials, cartilage stem/progenitor cells (CSPCs) derived from cartilage tissue present a promising choice for cartilage regeneration. In our previous study, we investigated whether CSPCs could be ideal seeding cells for cartilage tissue regeneration. Biomaterials are fabricated to accelerate tissue regeneration, providing a suitable environment for cell attachment, proliferation, and differentiation. Among the biomaterials used in cartilage regeneration medicine, alginate and collagen are classified as natural biomaterials and are characterized by high biocompatibility, bioactivity, and non-toxic degradation products. However, it is unclear which material would have a competitive advantage in CSPC-based cartilage regeneration in vivo. In the present study, we employed alginate and type Ⅰ collagen as substrates for CSPCs and chondrocytes, which was made control group, to explore a more suitable biomaterials for CSPCs to fabricate tissue-engineered cartilage, in vivo. Hematoxylin and eosin (HE) staining, Safranin O, immunohistochemical assay, and quantitative real-time polymerase chain reaction (qRT-PCR) were used to evaluate the tissue-engineered cartilage in vivo. Compared with the alginate group, collagen enhanced the expression of cartilage-specific genes, such as ACAN, SOX9, and COLII, more markedly. Furthermore, the marker genes of expression, dedifferentiation, and hypertrophy, COLI and COLX, were downregulated in the collagen group. The results demonstrated that collagen as a substrate was superior to alginate in increasing the accumulation of cartilage-like ECM for CSPCs in vivo. In summary, compared with alginate, collagen hydrogel is an effective biomaterial for CSPC-based cartilage regeneration.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative study of alginate and type I collagen as biomaterials for cartilage stem/progenitor cells to construct tissue-engineered cartilage in vivo

Zhang

Qi²,

Chen³

et al. 2023

Front. Bioeng. Biotechnol.

Self Cite

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“…Additionally, increased miR-31 and let-7 expression in hypoxia-treated ADSC-EVs resulted in the activation of the protein kinase A system (PKA) signaling pathway in endothelial cells, thus inducing the expression of endogenous VEGF and VEGF-R and leading to enhanced angiogenesis [ 39 , 63 , 64 ]. The role of hypoxic ADSC-EVs in cartilage regeneration has also been evaluated; upregulation of chondrocyte-related gene expression in hypoxic ADSC-EVs was found to induce more cartilage matrix and proteoglycan production, and this study is expected to play an important role in cartilage tissue engineering [ 65 ]. Hypoxia is only one of the more classic pretreatment strategies, but there are many others, such as the addition of lipopolysaccharides [ 66 ], hydrogen peroxide [ 66 ], hydrogen peroxide [ 67 ], atorvastatin [ 68 ], and pioglitazone [ 69 ].…”

Section: Extracellular Vesiclesmentioning

confidence: 99%

Extracellular vesicle-loaded hydrogels for tissue repair and regeneration

et al. 2023

Materials Today Bio

125

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“… 125 However, apparently few differences were found between ADSC‐EVs and BMSC‐EVs in the study of Gorgun et al, 126 and both of them could possess immunomodulatory, pro‐regenerative, pro‐angiogenic, and anti‐apoptotic properties. In the study of Xue et al, 127 the hypoxic ADSCs‐derived EVs improved the proliferation and chondrogenic differentiation of CPCs for cartilage tissue engineering. These results indicate that the ADSCs not only play a role in cartilage tissue engineering as seed cells but also serve as the source of exosomes, a regulator promoting cartilage regeneration.…”

Section: Chondrogenic Culture Of Adscsmentioning

confidence: 99%

Cartilage 3D bioprinting for rhinoplasty using adipose‐derived stem cells as seed cells: Review and recent advances

et al. 2023

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Nasal deformities due to various causes affect the aesthetics and use of the nose, in which case rhinoplasty is necessary. However, the lack of cartilage for grafting has been a major problem and tissue engineering seems to be a promising solution. 3D bioprinting has become one of the most advanced tissue engineering methods. To construct ideal cartilage, bio-ink, seed cells, growth factors and other methods to promote chondrogenesis should be considered and weighed carefully. With continuous progress in the field, bio-ink choices are becoming increasingly abundant, from a single hydrogel to a combination of hydrogels with various characteristics, and more 3D bioprinting methods are also emerging. Adipose-derived stem cells (ADSCs) have become one of the most popular seed cells in cartilage 3D bioprinting, owing to their abundance, excellent proliferative potential, minimal morbidity during harvest and lack of ethical considerations limitations. In addition, the coculture of ADSCs and chondrocytes is commonly used to achieve better chondrogenesis. To promote chondrogenic differentiation of ADSCs and construct ideal highly bionic tissue-engineered cartilage, researchers have used a variety of methods, including adding appropriate growth factors, applying biomechanical stimuli and reducing oxygen tension. According to the process and sequence of cartilage 3D bioprinting, this review summarizes and discusses the selection of hydrogel and seed cells (centered on ADSCs), the design of printing, and methods for inducing the chondrogenesis of ADSCs. | INTRODUCTIONTrauma, burn, tumour, surgery, or congenital malformation of nasal cartilage may compromise a nasal deformity or lead to nasal airway dysfunction, affecting the aesthetics and utility of the nose. 1 Rhinoplasty usually requires trimming of nasal cartilage and implantation of grafts. Therefore, finding the most suitable and compatible cartilage graft material is essential. Ideally, the engineered cartilage graft should have properties to meet the clinical needs of the recipient site, including being able to withstand the external forces in the nasal reconstructive environment, biocompatible, and biologically active to support and promote tissue integration and healing. Besides, it should be readily available or easy to access without contributing to donor site morbidity. Moreover, from a surgical point of view, the graft material needs to be big enough in the case of intraoperative trimming to meet the needs of patients.Chong Zhang and Guanhuier Wang contributed equally to this work.

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Hypoxic ADSCs-derived EVs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cells

Cited by 13 publications

References 59 publications

Comparative study of alginate and type I collagen as biomaterials for cartilage stem/progenitor cells to construct tissue-engineered cartilage in vivo

Comparative study of alginate and type I collagen as biomaterials for cartilage stem/progenitor cells to construct tissue-engineered cartilage in vivo

Extracellular vesicle-loaded hydrogels for tissue repair and regeneration

Cartilage 3D bioprinting for rhinoplasty using adipose‐derived stem cells as seed cells: Review and recent advances

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