Hypoxia tolerance in a selectively bred F4 population of blunt snout bream (Megalobrama amblycephala) under hypoxic stress

Wu, Chen; Zheng, Guo‐Dong; Zhao, Xinyu; Zhou, Shenjie; Zou, Shu‐Ming

doi:10.1016/j.aquaculture.2019.734484

Cited by 23 publications

(15 citation statements)

References 41 publications

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“…Blunt snout bream has always been considered as a hypoxia-sensitive species [ 2 , 4 , 37 , 38 ]. The experimental lethal DO value of blunt snout bream was 1.0 ± 0.5 mg·L − 1 (water temperature 20-25 °C) [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…The experimental lethal DO value of blunt snout bream was 1.0 ± 0.5 mg·L − 1 (water temperature 20-25 °C) [ 39 , 40 ]. While the experimental lethal DO value of “Pujiang No.2” was dropped below 0.90 mg·L − 1 (Water temperature 25 °C) [ 4 ]. Therefore, “Pujiang No.2” has strong hypoxia tolerance and extremely high commercial value.…”

Section: Discussionmentioning

confidence: 99%

“…At 25 °C, the LOE crit of the control group was 0.85 ± 0.11 mg·L − 1 , similar to the previous study (0.89 ± 0.08 mg·L − 1 ), and which was lower than that of “Pujiang No. 1” at 25 °C (~ 1.03 mg·L − 1 ) [ 4 ]. This finding verified that “Pujiang No.…”

Section: Discussionmentioning

confidence: 99%

“…Blunt snout bream ( Megalobrama amblycephala ) is native to the affiliated lakes of the Yangtze River [ 1 , 2 ]. It is an herbivorous freshwater fish species with a high economic value and high disease resistance in China [ 3 , 4 ]. Blunt snout bream production output was more than 7.6 × 10 5 tons in 2019 [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…In 2020, “Pujiang No.2”, developed by Shanghai Ocean University, was awarded a new aquatic product variety certificate (Breed Registration Number: GS-01-002-2020). Compared with “Pujiang No.1”, “Pujiang No.2” has a 27% increase in hypoxia tolerance, and the key dissolved oxygen value of body imbalance (LOE crit , 25 °C) in the fingerling stage has dropped below 0.90 mg·L − 1 [ 4 ]. However, the related mechanisms remain unclear.…”

Section: Introductionmentioning

confidence: 99%

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The transcriptomic responses of blunt snout bream (Megalobrama amblycephala) to acute hypoxia stress alone, and in combination with bortezomib

Zhao

Pan

et al. 2022

BMC Genomics

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Background Blunt snout bream (Megalobrama amblycephala) is sensitive to hypoxia. A new blunt snout bream strain, “Pujiang No.2”, was developed to overcome this shortcoming. As a proteasome inhibitor, bortezomib (PS-341) has been shown to affect the adaptation of cells to a hypoxic environment. In the present study, bortezomib was used to explore the hypoxia adaptation mechanism of “Pujiang No.2”. We examined how acute hypoxia alone (hypoxia-treated, HN: 1.0 mg·L− 1), and in combination with bortezomib (hypoxia-bortezomib-treated, HB: Use 1 mg bortezomib for 1 kg fish), impacted the hepatic ultrastructure and transcriptome expression compared to control fish (normoxia-treated, NN). Results Hypoxia tolerance was significantly decreased in the bortezomib-treated group (LOEcrit, loss of equilibrium, 1.11 mg·L− 1 and 1.32 mg·L− 1) compared to the control group (LOEcrit, 0.73 mg·L− 1 and 0.85 mg·L− 1). The HB group had more severe liver injury than the HN group. Specifically, the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the HB group (52.16 U/gprot, 32 U/gprot) were significantly (p < 0.01) higher than those in the HN group (32.85 U/gprot, 21. 68 U/gprot). In addition, more severe liver damage such as vacuoles, nuclear atrophy, and nuclear lysis were observed in the HB group. RNA-seq was performed on livers from the HN, HB and NN groups. KEGG pathway analysis disclosed that many DEGs (differently expressed genes) were enriched in the HIF-1, FOXO, MAPK, PI3K-Akt and AMPK signaling pathway and their downstream. Conclusion We explored the adaptation mechanism of “Pujiang No.2” to hypoxia stress by using bortezomib, and combined with transcriptome analysis, accurately captured the genes related to hypoxia tolerance advantage.

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