Hypoxia stimulates collagen hydroxylation in gingival fibroblasts and periodontal ligament cells

Morimoto, Chiaki; Takedachi, Masahide; Kawasaki, Kanako; Shimomura, Junpei; Murata, Mari; Hirai, Asae; Kawakami, Kazuma; Sawada, Kaichiro; Iwayama, Tomoaki; Murakami, Shinya

doi:10.1002/jper.20-0670

Cited by 14 publications

(15 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our enrichment analysis, we found that exposure to hypoxia was mainly associated with the intracellular component GO term, and P4HA1, which could be induced by HIF -1α or hypoxia-independent factors, is a part of a gene expression signature associated with hypoxia and glycolysis in ATII cells ( 59 , 60 ). P 4 HA 1 and P 4 HA 2 were the prolyl hydroxylase subtypes with significantly increased expression in cells under hypoxic conditions ( 61 – 63 ), which is similar to the results of our study, indicating that P4HA1 mediates hypoxia-induced invasion and migration ( 64 ).…”

Section: Discussionsupporting

confidence: 89%

Integrative Analysis of the lncRNA-Associated ceRNA Regulatory Network Response to Hypoxia in Alveolar Type II Epithelial Cells of Tibetan Pigs

Yang

Yuan

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

The function of alveolar type II epithelial (ATII) cells is severely hampered by oxygen deficiency, and understanding the regulatory mechanisms controlling responses to hypoxia may assist in relieving injury induced by hypoxia. In this study, we cultured ATII cells from Tibetan pigs and Landrace pigs under hypoxic and normoxic environments to screen for differentially expressed (DE) lncRNAs, DEmiRNAs, and construct their associated ceRNA regulatory networks in response to hypoxia. Enrichment analysis revealed that target genes of DElncRNAs of Tibetan pigs and Landrace pig between the normoxic (TN, LN) and hypoxic (TL, LL) groups significantly enriched in the proteoglycans in cancer, renal cell carcinoma, and erbB signaling pathways, while the target genes of DEmiRNAs were significantly enriched in the axon guidance, focal adhesion, and mitogen-activated protein kinase (MAPK) signaling pathways. Hypoxia induction was shown to potentially promote apoptosis by activating the focal adhesion/PI3K-Akt/glycolysis pathway. The ssc-miR-20b/MSTRG.57127.1/ssc-miR-7-5p axis potentially played a vital role in alleviating hypoxic injury by regulating ATII cell autophagy under normoxic and hypoxic conditions. MSTRG.14861.4-miR-11971-z-CCDC12, the most affected axis, regulated numerous RNAs and may thus regulate ATII cell growth in Tibetan pigs under hypoxic conditions. The ACTA1/ssc-miR-30c-3p/MSTRG.23871.1 axis is key for limiting ATII cell injury and improving dysfunction and fibrosis mediated by oxidative stress in Landrace pigs. Our findings provide a deeper understanding of the lncRNA/miRNA/mRNA regulatory mechanisms of Tibetan pigs under hypoxic conditions.

show abstract

Section: Discussionsupporting

confidence: 89%

Integrative Analysis of the lncRNA-Associated ceRNA Regulatory Network Response to Hypoxia in Alveolar Type II Epithelial Cells of Tibetan Pigs

Yang

Yuan

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…The results of qPCR showed that the relative expression of extracellular matrix gene products SPARC and type I collagen in all mice increased with the extension of tooth extraction time, however, the relative expression of them in the T1DM group were significantly lower. Type I collagen is the main structural component of the PDL, which maintains periodontal health ( 30 ). In previous orthodontic experiments, the expression of type I collagen increased during tooth movement ( 31 ).…”

Section: Discussionmentioning

confidence: 99%

“…which maintains periodontal health (30). In previous orthodontic experiments, the expression of type I collagen increased during tooth movement (31).…”

mentioning

confidence: 81%

Remodeling of the periodontal ligament and alveolar bone during axial tooth movement in mice with type 1 diabetes

Zheng

et al. 2023

Front. Endocrinol.

View full text Add to dashboard Cite

ObjectivesTo observe the elongation of the axial tooth movement in the unopposed rodent molar model with type 1 diabetes mellitus and explore the pathological changes of periodontal ligament and alveolar bone, and their correlation with tooth axial movement.MethodsThe 80 C57BL/6J mice were randomly divided into the streptozotocin(STZ)-injected group (n = 50) and the control group (n = 30). Mice in the streptozotocin(STZ)-injected group were injected intraperitoneal with streptozotocin (STZ), and mice in the control group were given intraperitoneal injection of equal doses of sodium citrate buffer. Thirty mice were randomly selected from the successful models as the T1DM group. The right maxillary molar teeth of mice were extracted under anesthesia, and allowed mandibular molars to super-erupt. Mice were sacrificed at 0, 3, 6,9, and 12 days. Tooth elongation and bone mineral density (BMD) were evaluated by micro-CT analysis(0,and 12 days mice). Conventional HE staining, Masson staining and TRAP staining were used to observe the changes in periodontal tissue(0, 3, 6, 9, and 12 days mice). The expression differences of SPARC, FGF9, BMP4, NOGGIN, and type I collagen were analyzed by RT-qPCR.ResultsAfter 12 days of tooth extraction, our data showed significant super-eruption of mandibular mouse molars of the two groups. The amount of molar super-eruption in the T1DM group was 0.055mm( ± 0.014mm), and in the control group was 0.157( ± 0.017mm). The elongation of the T1DM mice was less than that of the control mice(P<0.001). It was observed that the osteoclasts and BMD increased gradually in both groups over time. Compared with the control group, the collagen arrangement was more disordered, the number of osteoclasts was higher (P<0.05), and the increase of bone mineral density was lower(2.180 ± 0.007g/cm3 vs. 2.204 ± 0.006g/cm3, P<0.001) in the T1DM group. The relative expression of SPARC, FGF9, BMP4, and type I collagen in the two groups increased with the extension of tooth extraction time while NOGGIN decreased. The relative expression of all of SPARC, FGF9, BMP4, and type I collagen in the T1DM group were significantly lower, and the expression of NOGGIN was higher than that in the control group (P<0.05).ConclusionThe axial tooth movement was inhibited in type 1 diabetic mice. The result may be associated with the changes of periodontal ligament osteoclastogenic effects and alveolar bone remodeling regulated by the extracellular matrix and osteogenesis-related factors.

show abstract

“…Therefore, it is possible to selectively apply and eliminate only the wells in which the cells are cultured in pressure and low-oxygen environments. To change the existing cell culture environment from normoxia (20% O 2 concentration) [62] to hypoxia (1% O 2 concentration) [63], a wait of more than 2 h is required to stabilize the gas in the cell incubator [64] or to prepare a separate hypoxic incubator for replacement [65,66]. In addition, to apply pressure to the cells, financially burdensome hydrostatic pressure equipment [67,68] or mechanical loading device [20,69] are required.…”

Section: Discussionmentioning

confidence: 99%

Biomimetic hydrogel blanket for conserving and recovering intrinsic cell properties

Seo

et al. 2022

Biomater Res

View full text Add to dashboard Cite

Background Cells in the human body experience different growth environments and conditions, such as compressive pressure and oxygen concentrations, depending on the type and location of the tissue. Thus, a culture device that emulates the environment inside the body is required to study cells outside the body. Methods A blanket-type cell culture device (Direct Contact Pressing: DCP) was fabricated with an alginate-based hydrogel. Changes in cell morphology due to DCP pressure were observed using a phase contrast microscope. The changes in the oxygen permeability and pressure according to the hydrogel concentration of DCP were analyzed. To compare the effects of DCP with normal or artificial hypoxic cultures, cells were divided based on the culture technique: normal culture, DCP culture device, and artificial hypoxic environment. Changes in phenotype, genes, and glycosaminoglycan amounts according to each environment were evaluated. Based on this, the mechanism of each culture environment on the intrinsic properties of conserving chondrocytes was suggested. Results Chondrocytes live under pressure from the surrounding collagen tissue and experience a hypoxic environment because collagen inhibits oxygen permeability. By culturing the chondrocytes in a DCP environment, the capability of DCP to produce a low-oxygen and physical pressure environment was verified. When human primary chondrocytes, which require pressure and a low-oxygen environment during culture to maintain their innate properties, were cultured using the hydrogel blanket, the original shapes and properties of the chondrocytes were maintained. The intrinsic properties could be recovered even in aged cells that had lost their original cell properties. Conclusions A DCP culture method using a biomimetic hydrogel blanket provides cells with an adjustable physical pressure and a low-oxygen environment. Through this technique, we could maintain the original cellular phenotypes and intrinsic properties of human primary chondrocytes. The results of this study can be applied to other cells that require special pressure and oxygen concentration control to maintain their intrinsic properties. Additionally, this technique has the potential to be applied to the re-differentiation of cells that have lost their original properties.

show abstract

Hypoxia stimulates collagen hydroxylation in gingival fibroblasts and periodontal ligament cells

Cited by 14 publications

References 41 publications

Integrative Analysis of the lncRNA-Associated ceRNA Regulatory Network Response to Hypoxia in Alveolar Type II Epithelial Cells of Tibetan Pigs

Integrative Analysis of the lncRNA-Associated ceRNA Regulatory Network Response to Hypoxia in Alveolar Type II Epithelial Cells of Tibetan Pigs

Remodeling of the periodontal ligament and alveolar bone during axial tooth movement in mice with type 1 diabetes

Biomimetic hydrogel blanket for conserving and recovering intrinsic cell properties

Contact Info

Product

Resources

About