Hypoxia, reoxygenation and cytosolic manganese superoxide dismutase (cMnSOD) silencing in Litopenaeus vannamei: Effects on cMnSOD transcripts, superoxide dismutase activity and superoxide anion production capacity

García-Triana, Antonio; Zenteno‐Savín, Tania; Peregrino‐Uriarte, Alma B.; Yépiz-Plascencia, Glória

doi:10.1016/j.dci.2010.06.018

Cited by 67 publications

(36 citation statements)

References 31 publications

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“…An enzymatic AD system including the enzymes GPX, SOD and CAT is activated to reduce high ROS levels in the tissues (Kassahn et al 2009, García-Triana et al 2010, Sheikh zadeh et al 2012. Results of the current study show a significant downregulation in the expression of both antioxidant genes, cMnSOD and GPX, in shrimp co-culture.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have reported that the GPX and cMnSOD genes contribute significantly to GPX and SOD enzymatic activity and indicate its importance in the redox system regulation for L. vannamei (García-Triana et al 2010) and tilapia (Sheikh zadeh et al 2012). Expression of the cMnSOD gene was upregulated following exposure of L. vannamei to acute pH stress (Wang et al 2009) and hypoxia (García-Triana et al 2010). Activities of MnSOD and CuSOD in L. vannamei (Zhou et al 2010) and the abalone Haliotis discus (Kim et al 2007) have been shown to increase under heat stress.…”

Section: Introductionmentioning

confidence: 99%

“…To minimize the deleterious effects of ROS, shrimp possess an enzymatic and non-enzymatic antioxidant defence system (AD) which becomes more active in response to those stressors. Some of the most important antioxidant enzymes are glutathione peroxidase (GPX), superoxide dismutase (SOD) and catalase (CAT), which are necessary to reduce high ROS levels in the tissues (García-Triana et al 2010, Sheikhzadeh et al 2012. If excess ROS are not neutralized by the AD, oxidative stress-induced cellular damage and death may occur (Kassahn et al 2009).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antioxidant response and body composition of whiteleg shrimp co-cultured with Nile tilapia in recirculating aquaculture

Sharawy¹,

Thiele²,

Abbas³

et al. 2017

Aquacult. Environ. Interact.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antioxidant response and body composition of whiteleg shrimp co-cultured with Nile tilapia in recirculating aquaculture

Sharawy¹,

Thiele²,

Abbas³

et al. 2017

Aquacult. Environ. Interact.

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“…Previous studies have shown that mMnSOD and cMnSOD are induced in response to pathogen infection and environmental stress in marine invertebrate animals, and their expressions are signifi cantly induced in shrimps in response to bacteria Lin et al, 2010), viruses (Gómez-Anduro et al, 2006;Zhang et al, 2007), and changes in pH (Wang et al, 2009) and dissolved oxygen levels (García-Triana et al, 2010). However, little is known about the roles of mMnSOD and cMnSOD in the ammonia stress response of penaeid species.…”

Section: Introductionmentioning

confidence: 98%

Transcript profiles of mitochondrial and cytoplasmic manganese superoxide dismutases in Exopalaemon carinicauda under ammonia stress

Ren

Ping

et al. 2015

Chin. J. Ocean. Limnol.

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A bstractSuperoxide dismutase (SOD) is one of the most important antioxidant defense enzymes, and is considered as the fi rst line against oxidative stress. In this study, we cloned a mitochondrial manganese (Mn) SOD ( m MnSOD ) cDNA from the ridgetail white prawn Exopalaemon carinicauda by using rapid amplifi cation of cDNA ends (RACE) methods. The full-length cDNA for mMnSOD was 1 014-bp long, containing a 5′-untranslated region (UTR) of 37-bp, a 3′-UTR of 321-bp with a poly (A) tail, and included a 657-bp open reading frame encoding a protein of 218 amino acids with a 16-amino-acid signal peptide. The protein had a calculated molecular weight of 23.87 kDa and a theoretical isoelectric point of 6.75. The m MnSOD sequence included two putative N-glycosylation sites (NHT and NLS), the MnSOD signature sequence 180 DVWEHAYY 187 , and four putative Mn binding sites (H48, H96, D180, and H184). Sequence comparison showed that the mMnSOD deduced amino acid sequence of E . carinicauda shared 97%, 95%, 89%, 84%, 82%, 72%, and 69% identity with that of Macrobrachium rosenbergii , M acrobrachium nipponense , Fenneropeneaus chinensis , Callinectes sapidus , P erisesarma b idens , D anio r erio , and Homo sapiens , resectively. Quantitative real-time RT-PCR analysis showed that mMnSOD transcripts were present in all E . carinicauda tissues examined, with the highest levels in the hepatopancreas. During an ammonia stress treatment, the transcript levels of mMnSOD and cMnSOD were up-regulated at 12 h in hemocytes and at 24 h in the hepatopancreas. As the duration of the ammonia stress treatment extended to 72 h, the transcript levels of mMnSOD and cMnSOD signifi cantly decreased both in hemocytes and hepatopancreas. These fi ndings indicate that the SOD system is induced to respond to acute ammonia stress, and may be involved in environmental stress responses in E . carinicauda .

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“…The SOD activities were established by the degree of reaction inhibition [35]. The detailed method was as follows: soluble proteins (90 μL; protein extraction as above) were homogenized into 936 μL of a solution composed of 510 μL xanthine (1.5 mM), 6 μL XO (5 U/mL), 180 μL INT (0.45 mM), and 240 μL potassium phosphate buffer (300 mM, pH 7.8).…”

Section: Antioxidant Enzyme Assaysmentioning

confidence: 99%

Physiological adaptations to osmotic stress and characterization of a polyethylene glycol-responsive gene in Braya humilis

Wang

Zhao

et al. 2016

Acta Soc Bot Pol

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Braya humilis (Brassicaceae) is a widely distributed plant in arid and semi-arid regions of northern Asia. This plant is well adapted to extremely arid conditions and is a promising candidate species to discover novel drought tolerance strategies. However, not much information about the mechanism(s) mediating drought resistance in this species is currently available. Therefore, the present study aimed to characterize the physiological traits and expression patterns of a polyethylene glycol (PEG)-responsive gene in B. humilis responding to different levels of osmotic stress induced by PEG-6000. Several important physiological parameters were examined, including the levels of relative water content, soluble protein, malondialdehyde, and antioxidant enzyme activity. A tolerance threshold between 20 and 30% PEG-6000 was identified for B. humilis. The water status and oxidative damage below this threshold were maintained at a relatively constant level during the 12 h of treatment. However, once the threshold was exceeded, the water status and oxidative damage were obviously affected after treatment for 4 h. The soluble protein results suggest that B. humilis maintains a vigorous resistance to osmotic stress and that it may play a greater role in osmotic regulation at late stages of stress. Moreover, superoxide dismutase and catalase may be important at preventing oxidative damage in plants at early stages of stress, while peroxidase may be more involved in some biological processes that resist osmotic stress at the late stage, especially in severely damaged plants. Furthermore, a PEG-responsive gene, BhCIPK12, was identified by differential display reverse transcription-polymerase chain reaction (PCR), cloned, and characterized by quantitative real-time PCR. We hypothesized that this gene may play an important role in mediating osmotic stress or drought resistance in plants. Altogether, these results provide valuable insights into the mechanism(s) mediating drought tolerance in B. humilis.

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Hypoxia, reoxygenation and cytosolic manganese superoxide dismutase (cMnSOD) silencing in Litopenaeus vannamei: Effects on cMnSOD transcripts, superoxide dismutase activity and superoxide anion production capacity

Cited by 67 publications

References 31 publications

Antioxidant response and body composition of whiteleg shrimp co-cultured with Nile tilapia in recirculating aquaculture

Antioxidant response and body composition of whiteleg shrimp co-cultured with Nile tilapia in recirculating aquaculture

Transcript profiles of mitochondrial and cytoplasmic manganese superoxide dismutases in Exopalaemon carinicauda under ammonia stress

Physiological adaptations to osmotic stress and characterization of a polyethylene glycol-responsive gene in Braya humilis

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