Hypoxia reduces the osteogenic differentiation of peripheral blood mesenchymal stem cells by upregulating Notch-1 expression

Liu, Haixin; Wang, Yihan; Wu, Guofeng; Qiu, Sujun; Li, Chun; Tan, Zhiwen; Guo, Jiasong; Zhu, Lixin

doi:10.1080/03008207.2019.1611792

Cited by 25 publications

(21 citation statements)

References 51 publications

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“…Contrary to this, multilineage differentiation potential including osteogenic differentiation of tendon derived MSc was compromised in hypoxia cultures [76]. Minsheng Yang and colleagues found that 9% hypoxia increased osteogenesis whereas 1% results in decreased osteogenic potentials due to upregulation of Notch 1 expression [77]. Cells cultured throughout in hypoxic culture (5%) showed less osteogenic potential, less mineralization as compared to cells primed with hypoxia (5% for 7 days).…”

Section: Hypoxia Improves Differentiation Potential Of Cellsmentioning

confidence: 99%

Hypoxic Preconditioning as a Strategy to Maintain the Regenerative Potential of Mesenchymal Stem Cells

Bahir¹,

Choudhery²,

Hussain³

2020

Regenerative Medicine

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Mesenchymal stem cells (MSCs) are non-hematopoietic cells with high proliferative potential and multi-lineage differentiation capacity. MSCs are promising therapeutic candidates for cell-based therapies, and hundreds of clinical trials have been registered using these cells. Potential of stem cells is compromised with the factors such as disease condition and age of donor. Therefore, taking the cells from such patients for autologous use may compromise the benefits of cell-based therapies. It is therefore required to enhance the potential of these cells before use in stem cell-based therapies. Optimization of culture conditions is preferred strategies to enhance the regenerative potential of cells before use. This chapter briefly overviews the benefits of hypoxic preconditioning of stem cells to enhance the regenerative potential of cells in terms of their survival, proliferation, and differentiation.

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Section: Hypoxia Improves Differentiation Potential Of Cellsmentioning

confidence: 99%

Hypoxic Preconditioning as a Strategy to Maintain the Regenerative Potential of Mesenchymal Stem Cells

Bahir¹,

Choudhery²,

Hussain³

2020

Regenerative Medicine

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“…Egress was speculated to be associated with hypoxic conditions [159]. To date, the existence of short-lived bone marrow-derived peripheral blood circulating MSC is undoubted) [160][161][162]. However, MSC egress under physiological conditions requires more investigations.…”

Section: Mesenchymal Stem Cells General Backgroundmentioning

confidence: 99%

“…Cultures from mobilized peripheral blood progenitor cells of cancer patients or healthy donors yielded only a few adherent cells which showed fibroblast-like morphology, yet did not form bone in vivo [170]. The commonly reported frequency of MSC when mobilized was reported to be in range of 0.5% to 0.6% relative to all nucleated cells [162,165,166] Past reports concluded that isolation of MSC from non-mobilized peripheral blood was not successful [156,162]. However, this might not be correct.…”

Section: Circulating Rare Cell Population Concentrationsmentioning

confidence: 99%

“…Sielatycka et al [245] reported even 100 to 1000 cells in pregnant women using the CD45−/CD105+/CD90+/CD29+ phenotype. A concentration that exceeds 50 cells per mL seems unrealistic given the paucity and rarity of adherent cells suggesting that either most mesenchymal lineage fibroblast-like cells are non-adherent or including the possibility of analytical flaw or overlapping phenotypes [94,162]. We would also like to point out the issue of excluding the analysis of the cell nucleus in most flow cytometry methods.…”

Section: Circulating Rare Cell Population Concentrationsmentioning

confidence: 99%

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The Blood Circulating Rare Cell Population. What Is It and What Is It Good for?

Schreier

Triampo

2020

Cells

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Blood contains a diverse cell population of low concentration hematopoietic as well as non-hematopoietic cells. The majority of such rare cells may be bone marrow-derived progenitor and stem cells. This paucity of circulating rare cells, in particular in the peripheral circulation, has led many to believe that bone marrow as well as other organ-related cell egress into the circulation is a response to pathological conditions. Little is known about this, though an increasing body of literature can be found suggesting commonness of certain rare cell types in the peripheral blood under physiological conditions. Thus, the isolation and detection of circulating rare cells appears to be merely a technological problem. Knowledge about rare cell types that may circulate the blood stream will help to advance the field of cell-based liquid biopsy by supporting inter-platform comparability, making use of biological correct cutoffs and “mining” new biomarkers and combinations thereof in clinical diagnosis and therapy. Therefore, this review intends to lay ground for a comprehensive analysis of the peripheral blood rare cell population given the necessity to target a broader range of cell types for improved biomarker performance in cell-based liquid biopsy.

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“…The development of tissue engineering technology, involving three pivotal elements-seed cells, growth factors, and scaffold materials-has enhanced the treatment of several diseases [8]. Mesenchymal stem cells (MSCs) are the most commonly used seed cells in tissue engineering technology and include adipose-derived MSCs [9][10][11], peripheral blood-derived MSCs [12,13], and bone marrow MSCs (BMSCs) [14,15]. Owing to their unique advantages, BMSCs have become the most popular seed cells and are now widely used in many fields.…”

Section: Introductionmentioning

confidence: 99%

FGF-2-Induced Human Amniotic Mesenchymal Stem Cells Seeded on a Human Acellular Amniotic Membrane Scaffold Accelerated Tendon-to-Bone Healing in a Rabbit Extra-Articular Model

Zhang

Liu

et al. 2020

Stem Cells International

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Background. FGF-2 (basic fibroblast growth factor) has a positive effect on the proliferation and differentiation of many kinds of MSCs. Therefore, it represents an ideal molecule to facilitate tendon-to-bone healing. Nonetheless, no studies have investigated the application of FGF-2-induced human amniotic mesenchymal stem cells (hAMSCs) to accelerate tendon-to-bone healing in vivo. Objective. The purpose of this study was to explore the effect of FGF-2 on chondrogenic differentiation of hAMSCs in vitro and the effect of FGF-2-induced hAMSCs combined with a human acellular amniotic membrane (HAAM) scaffold on tendon-to-bone healing in vivo. Methods. In vitro, hAMSCs were transfected with a lentivirus carrying the FGF-2 gene, and the potential for chondrogenic differentiation of hAMSCs induced by the FGF-2 gene was assessed using immunofluorescence and toluidine blue (TB) staining. HAAM scaffold was prepared, and hematoxylin and eosin (HE) staining and scanning electron microscopy (SEM) were used to observe the microstructure of the HAAM scaffold. hAMSCs transfected with and without FGF-2 were seeded on the HAAM scaffold at a density of 3 × 10 5 cells/well. Immunofluorescence staining of vimentin and phalloidin staining were used to confirm cell adherence and growth on the HAAM scaffold. In vivo, the rabbit extra-articular tendon-to-bone healing model was created using the right hind limb of 40 New Zealand White rabbits. Grafts mimicking tendon-to-bone interface (TBI) injury were created and subjected to treatment with the HAAM scaffold loaded with FGF-2induced hAMSCs, HAAM scaffold loaded with hAMSCs only, HAAM scaffold, and no special treatment. Macroscopic observation, imageological analysis, histological assessment, and biomechanical analysis were conducted to evaluate tendon-tobone healing after 3 months. Results. In vitro, cartilage-specific marker staining was positive for the FGF-2 overexpression group. The HAAM scaffold displayed a netted structure and mass extracellular matrix structure. hAMSCs or hAMSCs transfected with FGF-2 survived on the HAAM scaffold and grew well. In vivo, the group treated with HAAM scaffold loaded with FGF-2-induced hAMSCs had the narrowest bone tunnel after three months as compared with other groups. In addition, macroscopic and histological scores were higher for this group than for the other groups, along with the best mechanical strength. Conclusion. hAMSCs transfected with FGF-2 combined with the HAAM scaffold could accelerate tendon-to-bone healing in a rabbit extraarticular model.

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Hypoxia reduces the osteogenic differentiation of peripheral blood mesenchymal stem cells by upregulating Notch-1 expression

Cited by 25 publications

References 51 publications

Hypoxic Preconditioning as a Strategy to Maintain the Regenerative Potential of Mesenchymal Stem Cells

Hypoxic Preconditioning as a Strategy to Maintain the Regenerative Potential of Mesenchymal Stem Cells

The Blood Circulating Rare Cell Population. What Is It and What Is It Good for?

FGF-2-Induced Human Amniotic Mesenchymal Stem Cells Seeded on a Human Acellular Amniotic Membrane Scaffold Accelerated Tendon-to-Bone Healing in a Rabbit Extra-Articular Model

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