Hypoxia‐ischaemia model in the 7‐day‐old rat: possibilities and shortcomings

Abstract

M. Hypoxia-ischaemia model in the 7-day-old rat: possibilities and shortcomings. Acta Pzediatr 1997; Suppl422: 85-8. Stockholm. ISSN The Levene model in 7-day-old rats is the most often used model of hypoxia-ischaemia (HI) in immature animals. The rat central nervous system is immature at birth and corresponds neurodevelopmentally to the term human infant during the second postnatal week. The Levene model of HI differs from clinical asphyxia with respect to the unilateral distribution of brain injury and lack of multi-organ dysfunction. Furthermore, it does not allow cardiovascular monitoring or repeated blood sampling. On the other hand, the progressive nature of HI bears many similarities to birth asphyxia with regard to blood flow changes and cellular metabolic derangements. The model is well characterized, easy to carry out and the low cost allows inclusion of a sufficient number of animals for dose-response evaluation of neuroprotective agents. In addition, it provides the unique opportunity of long-term evaluation of neuropathological and functional outcome. 0 Hypoxia, i&ture rat, ischaemia, neonatal, review H Hagberg,

Recurrent hypoinsulinemic hyperglycemia in neonatal rats increases PARP-1 and NF-κB expression and leads to microglial activation in the cerebral cortex

“…The anatomy of neonatal rat and human cerebral cortices is also an important difference. However, due to similarities in the rate of postnatal brain growth, development pattern of the germinal matrix, and neurochemical data, P7-P14 is generally accepted to correspond to a term human brain (39). Our model of recurring hyperglycemia is meant to affect the premature rat brain (<7 d), but also include the period of rapidly increasing glucose utilization between P7 and P12.…”

Prolonged Seizures Exacerbate Perinatal Hypoxic-Ischemic Brain Damage

“…As indicated above, no differences were noted among the experimental and control groups, suggesting that in the presence of wellpreserved cerebral blood flow, and presumably substrate delivery, seizures of this duration do not appear to cause direct neuronal damage. Finally, of the available models, the cerebral development of the postnatal d 10 rat pup appears to most closely parallel the human term infant (5,29,30). Although many studies have used rat pups Ն15 d of age, cerebral development at this age more likely parallels that of a more mature human infant rather than a neonate.…”

X-Linked Inhibitor of Apoptosis Protein Expression After Ischemic Injury in the Human and Rat Developing Brain

“…Because of all these limitations in using human tissue, we investigated the temporal and spatial expression of XIAP in a more controlled experimental paradigm using the RiceVannucci rat model of neonatal stroke. The brain maturity of a 7-d-old rat used in this model corresponds to a brain of human term infant (26).…”