Hypoxia inhibits human bladder smooth muscle cell proliferation: A potential mechanism of bladder dysfunction

Galvin, David; Watson, R. W. G.; O’Neill, Amanda; Coffey, Ronan; Taylor, Cormac T.; Gillespie, James; Fitzpatrick, John M.

doi:10.1002/nau.20035

Cited by 26 publications

(33 citation statements)

References 18 publications

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“…The response of human detrusor muscle to hypoxic insult was investigated in an in vitro cell culture model [9]. Those authors reported a time-dependent increase in HIF-1 α expression (up to 72 h) with a doubling of vascular endothelial growth factor (VEGF), while spontaneous cell apoptosis and viability were not influenced.…”

Section: Discussionmentioning

confidence: 99%

“…LUTS were classified according to the IPSS as minimal (score 0-7), moderate (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) and severe ( > 20). The duration of LUTS was also recorded .…”

Section: Methodsmentioning

confidence: 99%

“…In humans the development of BOO is very insidious and progresses slowly, so the different stages of bladder deterioration cannot be fully investigated [4], thus there have been few reports to examine hypoxia in the obstructed human detrusor [9,10]. However, in vitro models were developed, and strips of detrusor smooth muscle used to investigate how BOO affects detrusor physiology [9].…”

Section: Introductionmentioning

confidence: 99%

“…However, in vitro models were developed, and strips of detrusor smooth muscle used to investigate how BOO affects detrusor physiology [9]. Recently, with the use of laser Doppler ultrasonography (US), bladder blood flow was found to decrease during the later stages of filling, along with the increase in intravesical pressure [11].…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical estimation of hypoxia in human obstructed bladder and correlation with clinical variables

Koritsiadis¹,

Stravodimos²,

Koutalellis³

et al. 2008

BJU International

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OBJECTIVETo investigate the tissue distribution of ischaemia in human detrusor in patients with bladder outlet obstruction (BOO) and to correlate the results with clinical variables, as clinical BOO is a common problem in ageing men and ischaemia might be important in detrusor dysfunction.PATIENTS AND METHODSFrom September 2004 to October 2006, 70 patients were recruited, comprising 60 scheduled for surgery to treat benign prostatic hyperplasia (the study group) and 10 as controls. Detrusor tissue was retrieved and stained for hypoxia‐inducible factor (HIF)‐1α, a cellular marker of hypoxia.RESULTSThe mean (sd) total number of cells immunoreactive to HIF‐1α in the study group was 93.3 (48.09), and in the specimens from the control group only few rare cells showed weak immunoreactivity to HIF‐1α (0–2). Positive cells were in different proportions between muscle bundles and submucosa, expressed mainly in stromal cells. The urothelium and detrusor muscle showed no immunoreactivity to HIF‐1α. There was strong immunoreactivity in patients with prolonged BOO (<10 years), declining thereafter, and in those patients with urinary retention.CONCLUSIONSThe urothelium and detrusor seem to be more resistant to hypoxic stress, while stromal cells perceive low oxygen tension. The bladder response to chronic hypoxia through HIF‐1α expression is limited in time and might depend on the functional status of the detrusor.

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Section: Discussionmentioning

confidence: 99%

“…LUTS were classified according to the IPSS as minimal (score 0-7), moderate (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) and severe ( > 20). The duration of LUTS was also recorded .…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Immunohistochemical estimation of hypoxia in human obstructed bladder and correlation with clinical variables

Koritsiadis¹,

Stravodimos²,

Koutalellis³

et al. 2008

BJU International

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show abstract

“…There are some data to support the importance of hypoxia in LUTS, 85 although there is debate regarding potential mechanisms. 86 It is also reasonable to generalize the concept to incorporate other forms of vascular compromise that may contribute to hypoxia or other local perfusion-induced anatomical or functional problems. For instance, measures of perfusion have shown vascular changes in prostate and corpora cavernosa, suggesting disease common to both sites.…”

Section: Pelvic Atherosclerosismentioning

confidence: 99%

A survey of commonalities relevant to function and dysfunction in pelvic and sexual organs

2007

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Micturition, defecation and sexual function are all programmed through spinal reflexes that are under descending control from higher centres. Interaction between these reflexes can clearly be perceived, and evidence is accumulating the dysfunction in one reflex is often associated with dysfunction in another. In this article, we describe some of the basic properties and neural control of the smooth muscles mediating the reflexes, reviewing the common features that underlie these reflex functions, and what changes may be responsible for dysfunction. We propose that autonomic control within the pelvis predisposes pelvic and sexual organs to crosstalk, with the consequence that diseases and conditions of the pelvis are subject to convergence on a functional level. It should be expected that disturbance of the function of one system will inevitably impact adjacent systems.

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Do functional changes occur in the bladder due to bladder outlet obstruction? ‐ ICI‐RS 2018

Bosch

Abrams

Averbeck

et al. 2019

Neurourology and Urodynamics

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Studies on bladder dysfunction (BD), more specifically functional‐urodynamic changes in the bladder as a result of bladder outlet obstruction (BOO) have been summarized for this TT. Based on available, but limited evidence from human studies a three‐stage model can be hypothesized to characterize BOO‐induced bladder remodeling: hypertrophy, compensation (increased detrusor contractility during the voiding phase, often in combination with filling phase detrusor overactivity) followed by the phase of decompensation [detrusor underactivity]. The time between the start of compensation and eventual decompensation seems to be determined by age of onset, severity, and type of obstruction and clinical mitigating factors such as vascular and metabolic problems. Understanding the relative contributions of these factors may allow the development of personalized timelines and probabilities for these obstructed patients.

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Hypoxia inhibits human bladder smooth muscle cell proliferation: A potential mechanism of bladder dysfunction

Cited by 26 publications

References 18 publications

Immunohistochemical estimation of hypoxia in human obstructed bladder and correlation with clinical variables

Immunohistochemical estimation of hypoxia in human obstructed bladder and correlation with clinical variables

A survey of commonalities relevant to function and dysfunction in pelvic and sexual organs

Do functional changes occur in the bladder due to bladder outlet obstruction? ‐ ICI‐RS 2018

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