Hypoxia‐inducible factor‐1alpha and nitric oxide synthases in bovine follicles close to ovulation and early luteal angiogenesis

Berisha, Bajram; Schams, D.; Sinowatz, Fred; Rodler, Daniela; Pfaffl, Michael W.

doi:10.1111/rda.13812

Cited by 5 publications

(6 citation statements)

References 78 publications

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“…NO-signaling is known in mammalian ovarian physiology, as it has been demonstrated that the multiple isoenzymes of nitric oxide synthase (NOS) are expressed and localized in the ovary of different species [ 65 , 66 ]. A role for NO was ascribed to the processes of steroidogenesis, folliculogenesis, oocyte meiotic maturation, and ovulation [ 65 , 67 , 68 ]. SODs in mammals are known to modulate the bioavailability of nitric oxide and peroxynitrite (ONOO-) rate at vascular smooth muscles or, hence, in the extracellular space [ 49 , 69 ].…”

Section: Discussionmentioning

confidence: 99%

Orthologs of NOX5 and EC-SOD/SOD3: dNox and dSod3 Impact Egg Hardening Process and Egg Laying in Reproductive Function of Drosophila melanogaster

Steinmetz,

Scherer,

Calvet

et al. 2024

IJMS

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The occurrence of ovarian dysfunction is often due to the imbalance between the formation of reactive oxygen species (ROS) and the ineffectiveness of the antioxidative defense mechanisms. Primary sources of ROS are respiratory electron transfer and the activity of NADPH oxidases (NOX) while superoxide dismutases (SOD) are the main key regulators that control the levels of ROS and reactive nitrogen species intra- and extracellularly. Because of their central role SODs are the subject of research on human ovarian dysfunction but sample acquisition is low. The high degree of cellular and molecular similarity between Drosophila melanogaster ovaries and human ovaries provides this model organism with the best conditions for analyzing the role of ROS during ovarian function. In this study we clarify the localization of the ROS-producing enzyme dNox within the ovaries of Drosophila melanogaster and by a tissue-specific knockdown we show that dNox-derived ROS are involved in the chorion hardening process. Furthermore, we analyze the dSod3 localization and show that reduced activity of dSod3 impacts egg-laying behavior but not the chorion hardening process.

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Section: Discussionmentioning

confidence: 99%

Orthologs of NOX5 and EC-SOD/SOD3: dNox and dSod3 Impact Egg Hardening Process and Egg Laying in Reproductive Function of Drosophila melanogaster

Steinmetz,

Scherer,

Calvet

et al. 2024

IJMS

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“…One microgram of total RNA was reverse‐transcribed to cDNA using the master mix of Promega, Random Primers (Invitrogen), dNTPs (Fermentas) and M‐MLV Reverse Transcriptase (Promega) according to our previous study (Berisha et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

“…The RT‐qPCR protocol for all investigated genes (Table 1) was done using a RotorGene 3000 (Corbett Research) according to Berisha et al (2020).…”

Section: Methodsmentioning

confidence: 99%

Regulatory changes of local produced prostaglandins in corpus luteum after experimentally induced luteolysis in the cow

Berisha

Thaqi

Rodler

et al. 2022

Anat Histol Embryol

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The objective of the study was to evaluate the expression patterns of prostaglandin F2alpha (PGF), prostaglandin E2 (PGE), PGF receptor (FP), PGE receptors (EP2 and EP4), prostaglandin‐endoperoxide synthase 2 (PTGS2) and prostaglandin synthases (PGFS and PGES) in corpora lutea (CL) during experimentally induced luteolysis in cow. The Fleckvieh cows in the mid‐luteal phase (days 8–12, control group) were injected with cloprostenol (PGF analogue), and CL were collected by transvaginal ovariectomy before (days 8–12, control group) and at 0.5, 2, 4, 12, 24, 48 and 64 h after PGF application (n = 5 per group). The mRNA expression was determined by RT‐qPCR, the hormone concentrations by enzyme immunoassay and localization by immunohistochemistry. PTGS2 gene expression increased significantly 2 h after PGF application, followed by continuous and significant downregulation afterwards. The PGF tissue concentration increased significantly just after PGF injection and again during structural luteolysis (after 12 h), whereas PGE concentration significantly decreased during structural luteolysis. The FP receptor mRNA decreased significantly at 2 h and again at 12 h after PGF. In contrast, EP4 receptor mRNA increased significantly just after the PGF application (0.5 h). The immunostaining of PGES and PTGS2 on day 15–17 shows numerous positive luteal cells, followed by lower activity afterwards on day 18 (luteolysis). In conclusion, the changes of examined prostaglandin family members in CL tissue after PGF application may be key components of the local mechanisms regulating the cascade of actions leading to functional and subsequent structural luteolysis in the bovine ovary.

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“…Recognizing the undoubted importance of above-mentioned mediators in reproduction processes, our research group focused on the main phases of ovarian function, using different experimental models and analytical methods (Berisha et al, 2019(Berisha et al, , 2020(Berisha et al, , 2022Livak & Schmittgen, 2001;Mueller et al, 2004).…”

Section: Pros Tag L and In S And Ovarian Fun C Tionmentioning

confidence: 99%

“…Here, we can mention the essential role of prostaglandins during the ovarian cycle, endometrial receptivity, embryo implantation, pregnancy, parturition and postpartum stage (Weems et al, 2006). Recognizing the undoubted importance of above‐mentioned mediators in reproduction processes, our research group focused on the main phases of ovarian function, using different experimental models and analytical methods (Berisha et al, 2019, 2020, 2022; Livak & Schmittgen, 2001; Mueller et al, 2004).…”

Section: Prostaglandins and Ovarian Functionmentioning

confidence: 99%

Prostaglandins as local regulators of ovarian physiology in ruminants

Berisha,

Thaqi,

Sinowatz

et al. 2023

Anat Histol Embryol

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Prostaglandins are synthesized from arachidonic acid through the catalytic activities of cyclooxygenase, while the production of different prostaglandin types, prostaglandin F2 alpha (PGF) and prostaglandin E2 (PGE), are regulated by specific prostaglandin synthases (PGFS and PGES). Prostaglandin ligands (PGF and PGE) bind to specific high‐affinity receptors and initiate biologically distinct signalling pathways. In the ovaries, prostaglandins are known to be important endocrine regulators of female reproduction, in addition to maintaining local function through autocrine and/or paracrine effect. Many research groups in different animal species have already identified a variety of factors and molecular mechanisms that are responsible for the regulation of prostaglandin functions. In addition, prostaglandins stimulate their intrafollicular and intraluteal production via the pathway of prostaglandin self‐regulation in the ovary. Therefore, the objective of the review article is to discuss recent findings about local regulation patterns of prostaglandin ligands PGF and PGE during different physiological stages of ovarian function in domestic ruminants, especially in bovine. In conclusion, the discussed local regulation mechanisms of prostaglandins in the ovary may stimulate further research activities in different methodological approaches, especially during final follicle maturation and ovulation, as well as corpus luteum formation and function.

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Hypoxia‐inducible factor‐1alpha and nitric oxide synthases in bovine follicles close to ovulation and early luteal angiogenesis

Abstract: The gonadotropin surge in ruminants activates a biochemical cascade that leads to the final follicle growth and ovulation, resulting in corpus luteum (CL) formation and

Cited by 5 publications

References 78 publications

Orthologs of NOX5 and EC-SOD/SOD3: dNox and dSod3 Impact Egg Hardening Process and Egg Laying in Reproductive Function of Drosophila melanogaster

Orthologs of NOX5 and EC-SOD/SOD3: dNox and dSod3 Impact Egg Hardening Process and Egg Laying in Reproductive Function of Drosophila melanogaster

Regulatory changes of local produced prostaglandins in corpus luteum after experimentally induced luteolysis in the cow

Prostaglandins as local regulators of ovarian physiology in ruminants

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