Hypoxia-induced miR-210 in epithelial ovarian cancer enhances cancer cell viability via promoting proliferation and inhibiting apoptosis

Abstract

miR-210 is upregulated in a HIF-1α-dependent way in several types of cancers. In addition, upregulated miR-210 promotes cancer proliferation, via its anti-apoptotic effects. It is blind to the regulation of miR-210 under hypoxia conditions for ovarian cancer cells and to the effect of miR-210 on ovarian cancer growth. In the present study, we determined the expression of miR-210 in epithelial ovarian cancer specimens, and in ovarian cancer cell lines under hypoxia conditions, and determined in detail the effect of miR-210 overexpression on tumor cell proliferation, and the possible mechanisms of tumor growth by miR-210 regulation. It was shown that miR-210 expression is upregulated, in response to hypoxia conditions in epithelial ovarian cancer specimens as well as epithelial ovarian cancer cell lines, with an association to HIF-1α overexpression. Furthermore, upregulated miR-210 promoted tumor growth in vitro via targeting PTPN1 and inhibiting apoptosis. Therefore, our findings shed light on the mechanism of ovarian cancer adaptation to hypoxia.

The circulating microRNA-200 family in whole blood are potential biomarkers for high-grade serous epithelial ovarian cancer

“…As a result, the miR-200 family may not only be used to detect and stage EOC but may provide information regarding prognosis. miR-210 has been shown to be increased in EOC tissue and cell lines in response to tissue hypoxia; however, little is known with regard to circulating miR-210 in EOC (10). miR-21 has been identified as an important oncogene in multiple types of cancer and has been shown to be increased in EOC tissue; nevertheless, little is known regarding circulating miR-21 in EOC (5,11).…”

Epithelial-to-Mesenchymal Transition in the Female Reproductive Tract: From Normal Functioning to Disease Pathology

“…In response to hypoxia, miR-210 is upregulated in OC tissue as well as OC cell lines, and miR-210 mediates hypoxia-induced EMT by promoting Snail expression leading to inhibition of E-cadherin transcription. Additionally, miR-210 was found to increase the transcriptional activity of HIF-1, suggesting that a positive feedback loop may exist between miR-210 and HIF-1 that reciprocally modulates miR-210 release and thus sustains the function of miR-210 under hypoxia (243). …”

microRNA-126 suppresses PAK4 expression in ovarian cancer SKOV3 cells

“…miR-126 is a non-coding RNA that is involved in various cellular processes, including proliferation, differentiation, apoptosis and invasion (17,21,29). miRNAs that are upregulated in cancer may function as oncogenes through the negative regulation of tumor suppressor genes, whilst miRNAs that are downregulated may function as tumor suppressor genes and inhibit cancer by regulating oncogenes (30,31). miR-126 acts as a metastatic suppressor in a number of human cancers (21,23).…”