Hypoxia-induced metabolic and antioxidant enzymatic activities in the estuarine fish Leiostomus xanthurus

Cooper, Rebecca U.; Clough, Lisa M.; Farwell, Mary A.; West, Terry L.

doi:10.1016/s0022-0981(02)00329-5

Cited by 182 publications

(100 citation statements)

References 48 publications

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“…LDH plasma levels have been used in toxicology and clinical biochemistry to diagnose lesions at the cellular, tissue, and organ level (Asztalos et al 1990;Gupta et al 1991). Alterations of normal LDH activity patterns have already been found in several fish species collected from polluted areas or exposed in situ (Castro et al 2004;Gül et al 2004), in fish exposed to crude oil (Gagnon and Holdway 1999) or subjected to hypoxic conditions (Cooper et al 2002). The increased LDH and CK plasma activities observed in the present study may indicate that more intense metabolism in fish muscles took place (increased glycolysis and formation of creatine phosphate; Marks et al 1996;Brightman et al 1997).…”

Section: Discussionmentioning

confidence: 99%

Biomarker responses in flounder Platichthys flesus from the Polish coastal area of the Baltic Sea and applications in biomonitoring

et al. 2009

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The objective of this study was to investigate the pattern of enzymatic activities, environmental genotoxicity and cytotoxicity in flounder, Platichthys flesus, from the Polish coastal area of the Baltic Sea. Fish were sampled in different contaminated sites in the Gulf of Gdansk and in a reference area outside the gulf. The activities of acetylcholinesterase (AChE), glutathione S: -transferase (GST), catalase (CAT), alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase (CK), lactate dehydrogenase (LDH), were studied, as well as the frequency of micronuclei, nuclear buds and fragmented-apoptotic cells. A higher mean activity level of muscular AChE and a lower activity level of hepatic GST were evident in samples taken from the reference site, relative to those found in the gulf. Modeled CAT activity (in both liver and gill tissue), blood plasma LDH and CK activities were all significantly higher in flounder collected at locations within the Gulf of Gdansk than at the reference site. No statistically significant alterations were observed in the activities of ALT and AST in the blood plasma of flounder in this study. Fish collected from a location at the mouth of the Vistula River showed the highest hepatic GST and CAT, the highest gill CAT activity, and the highest frequency of blood micronuclei, nuclear buds and fragmented-apoptotic cell inductions, as well as the lowest level of blood plasma CK. The present study confirms that compared to fish from the reference area, flounder from the Gulf of Gdansk clearly demonstrate a different enzyme activity, genotoxicity and cytotoxicity biomarker response pattern.

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Section: Discussionmentioning

confidence: 99%

Biomarker responses in flounder Platichthys flesus from the Polish coastal area of the Baltic Sea and applications in biomonitoring

et al. 2009

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“…This process was termed ''preparation for oxidative stress'' . Lushchak et al (2001), in goldfish, andCooper et al (2002), in the estuarine spot fish (Leiostomus xanthurus), showed that anoxia activated antioxidant defenses, in agreement with previous reports for other poikilotherms (Vig and Nemcsok, 1989;Storey, 1993a,b, 1998), and opposed to findings in mammals (Shlafer et al, 1987;Kirshenbaum and Singal, 1992;Singh et al, 1993). This response in fish is considered an evolutionary adaptation, playing a protective role during oxygen deficient conditions that allows the fish to cope with oxidative stress arising from tissue reoxygenation (for review see Hochachka and Lutz, 2001;Hermes-Lima and Zenteno-Savı´n, 2002).…”

Section: Antioxidant Defenses Fish Behavior and Environmental Factorsmentioning

confidence: 99%

Antioxidant Defenses in Fish: Biotic and Abiotic Factors

Martínez‐Álvarez

Morales

Sanz

2005

Rev Fish Biol Fisheries

961

456

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“…In the estuarine fish Leiostomus xanthurus, organ-specific increases in SOD activity were also observed under hypoxia (Cooper et al 2002). In addition, hypoxia exposure in Pacific oysters prompted increased GPX expression in the gills and mantle (David et al 2005).…”

Section: Oxidative Stress In Hypoxic/anoxic-tolerant Speciesmentioning

confidence: 99%