Hypoxia in skeletal muscles: from physiology to gene expression

Zhang, Gan

doi:10.14800/mr.1193

Cited by 2 publications

(2 citation statements)

References 107 publications

(149 reference statements)

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“…Under hypoxic conditions, formation of ROS induces oxidation of cellular components, including proteins, lipids and DNA, triggering cell damage [69]. One mechanism of removing the harmful products is via the glutathione detoxification pathway [70].…”

Section: Resultsmentioning

confidence: 99%

Absolute expressions of hypoxia-inducible factor-1 alpha (HIF1A) transcript and the associated genes in chicken skeletal muscle with white striping and wooden breast myopathies

et al. 2019

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Development of white striping (WS) and wooden breast (WB) in broiler breast meat have been linked to hypoxia, but their etiologies are not fully understood. This study aimed at investigating absolute expression of hypoxia-inducible factor-1 alpha subunit ( HIF1A ) and genes involved in stress responses and muscle repair using a droplet digital polymerase chain reaction. Total RNA was isolated from pectoralis major collected from male 6-week-old medium (carcass weight ≤ 2.5 kg) and heavy (carcass weight > 2.5 kg) broilers. Samples were classified as “non-defective” (n = 4), “medium-WS” (n = 6), “heavy-WS” (n = 7) and “heavy-WS+WB” (n = 3) based on abnormality scores. The HIF1A transcript was up-regulated in all of the abnormal groups. Transcript abundances of genes encoding 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 ( PFKFB4 ), lactate dehydrogenase-A ( LDHA ), and phosphorylase kinase beta subunit ( PHKB ) were increased in heavy-WS but decreased in heavy-WS+WB. Glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) was up-regulated in non-defective samples. The muscle-specific mu-2 isoform of glutathione S-transferases ( GSTM2 ) was up-regulated in the abnormal samples, particularly in the heavy groups. The genes encoding myogenic differentiation ( MYOD1 ) and myosin light chain kinase ( MYLK ) exhibited similar expression pattern, of which medium-WS and heavy-WS significantly increased compared to non-defective whereas expression in heavy-WS+WB was not different from either non-defective or WS-affected group. The greatest and the lowest levels of calpain-3 ( CAPN3 ) and delta-sarcoglycan ( SCGD ) were observed in heavy-WS and heavy-WS+WB, respectively. Based on micrographs, the abnormal muscles primarily comprised fibers with cross-sectional areas ranging from 2,000 to 3,000 μm 2 . Despite induced glycolysis at the transcriptional level, lower stored glycogen in the abnormal muscles corresponded with the reduced lactate and higher pH within their meats. The findings support hypoxia within the abnormal breasts, potentially associated with oversized muscle fibers. Between WS and WB, divergent glucose metabolism, cellular detoxification and myoregeneration at the transcriptional level could be anticipated.

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Section: Resultsmentioning

confidence: 99%

Absolute expressions of hypoxia-inducible factor-1 alpha (HIF1A) transcript and the associated genes in chicken skeletal muscle with white striping and wooden breast myopathies

et al. 2019

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“…Compared to other oxygen-sensitive tissues such as the brain and heart, skeletal muscles tolerate hypoxia quite well because of their plasticity. Indeed, they adapt their metabolism and structural features (fiber size, muscle fiber type, mitochondrial activity, myoglobin content) as well as blood supply (capillary density) in response to hypoxia, a condition muscles encounter in non-pathological contexts e.g., physical exercise or exposure to high altitude [13][14][15]. Obviously, muscle adaptation will strongly depend on exercise training duration and modalities (resistive vs. endurant).…”

Section: Introductionmentioning

confidence: 99%

Hypoxia and Hypoxia-Inducible Factor Signaling in Muscular Dystrophies: Cause and Consequences

Nguyen

Conotte

Belayew

et al. 2021

IJMS

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Muscular dystrophies (MDs) are a group of inherited degenerative muscle disorders characterized by a progressive skeletal muscle wasting. Respiratory impairments and subsequent hypoxemia are encountered in a significant subgroup of patients in almost all MD forms. In response to hypoxic stress, compensatory mechanisms are activated especially through Hypoxia-Inducible Factor 1 α (HIF-1α). In healthy muscle, hypoxia and HIF-1α activation are known to affect oxidative stress balance and metabolism. Recent evidence has also highlighted HIF-1α as a regulator of myogenesis and satellite cell function. However, the impact of HIF-1α pathway modifications in MDs remains to be investigated. Multifactorial pathological mechanisms could lead to HIF-1α activation in patient skeletal muscles. In addition to the genetic defect per se, respiratory failure or blood vessel alterations could modify hypoxia response pathways. Here, we will discuss the current knowledge about the hypoxia response pathway alterations in MDs and address whether such changes could influence MD pathophysiology.

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Hypoxia in skeletal muscles: from physiology to gene expression

Cited by 2 publications

References 107 publications

Absolute expressions of hypoxia-inducible factor-1 alpha (HIF1A) transcript and the associated genes in chicken skeletal muscle with white striping and wooden breast myopathies

Absolute expressions of hypoxia-inducible factor-1 alpha (HIF1A) transcript and the associated genes in chicken skeletal muscle with white striping and wooden breast myopathies

Hypoxia and Hypoxia-Inducible Factor Signaling in Muscular Dystrophies: Cause and Consequences

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