Hypoxia drives apoptosis independently of p53 and metallothionein transcript levels in hemocytes of the whiteleg shrimp Litopenaeus vannamei

Félix-Portillo, Monserrath; Martínez-Quintana, José A.; Arenas-Padilla, Marina; Mata-Haro, Verónica; Gómez‐Jiménez, Silvia; Yépiz-Plascencia, Glória

doi:10.1016/j.chemosphere.2016.07.041

Cited by 40 publications

(24 citation statements)

References 67 publications

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“…Previous studies have shown that hypoxia stress leads to increased expression of the p53 gene and p53 protein in oriental river prawn (Macrobrachium nipponense) and white shrimp (Litopenaeus vannamei) hepatopancreas and haemocytes, leading to apoptosis (Nuñez-Hernandez et al, 2018;Sun et al, 2016;Felix-Portillo et al, 2016). Similar results were found in this study.…”

Section: Discussionsupporting

confidence: 91%

Hypoxia-induced miR-92a regulates p53 signalling pathway and apoptosis by targeting calcium-sensing receptor in Genetically Improved Farmed Tilapia (Oreochromis niloticus)

Qiang

Tao

Bao

et al. 2020

Preprint

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AbstractmiR-92a miRNAs are immune molecules that regulate apoptosis (programmed cell death) during the immune response. Apoptosis helps to maintain the dynamic balance in tissues of fish under hypoxia stress. The aim of this study was to explore the role and potential mechanisms of miR-92a in the liver of tilapia under hypoxia stress. We first confirmed that CaSR (encoding a calcium-sensing receptor) is a target gene of miR-92a in genetically improved farmed tilapia (GIFT) using luciferase reporter gene assays. In GIFT under hypoxia stress, miR-92a was up-regulated and CaSR was down-regulated in a time-dependent manner. Knocked-down CaSR expression led to inhibited expression of p53, TP53INP1 and caspase-3/8, reduced the proportion of apoptotic hepatocytes, and decreased the activity of calcium ions induced by hypoxia in hepatocytes. GIFT injected in the tail vein with an miR-92a agomir showed up-regulation of miR-92a and down-regulation of CaSR, p53, TP53INP1, and caspase-3/8 genes in the liver, resulting in lower serum alanine aminotransferase and aspartate aminotransferase activities under hypoxia stress. These findings suggest that stimulation of miR-92a interferes with hypoxia-induced apoptosis in hepatocytes of GIFT by targeting CaSR, thereby alleviating liver damage. These results provide new insights into the adaptation mechanisms of GIFT to hypoxia stress.

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Section: Discussionsupporting

confidence: 91%

Hypoxia-induced miR-92a regulates p53 signalling pathway and apoptosis by targeting calcium-sensing receptor in Genetically Improved Farmed Tilapia (Oreochromis niloticus)

Qiang

Tao

Bao

et al. 2020

Preprint

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“…KX827273). LvFLp53 cDNA sequence was identical to the previous reported p53 from L. vannamei (KC422442.1 or KX179650) 29 30 with a little discrepancy in the coding region, which may be generated by synonymous or nonsynonymous substitution. The full length of LvΔNp53 cDNA (KX827274) was 1818 bp in size containing an ORF of 948 bp that encoded a polypeptide of 315 amino acids with MW of ~34.8 kDa and theoretical pI of ~8.33 ( Supplement Fig.…”

Section: Resultssupporting

confidence: 62%

“…A growing number of studies support the idea that p53-mediated cell response needs to be considered as isoform-specific and is, in fact, the sum of the activities of the coexpressed p53 isoforms 2 . In shrimp, p53 is reported to be implicated with host survival via its regulation roles on MnSOD and GPx in response to acute environmental stresses 29 , and p53 also plays an important role in apoptosis in response to hypoxia 30 , nevertheless, the contribution of shrimp p53 isoforms mediated immune response to pathogenic infection still remains unrevealed. In the current study, we cloned a novel alternatively spliced form of p53 from L. vannamei named as LvΔNp53, and then evaluated the regulatory roles of LvΔNp53 and LvFLp53 (full length of p53) on host immune genes and their function during viral infection.…”

Section: Discussionmentioning

confidence: 99%

“…However, it may be a different situation during virus infection. In addition to that LvFLp53 can suppress the expression of WSSV IEs by targeting with NF-κB (Dorsal), L. vannamei p53 (sequence identical to LvFLp53) has been reported to play a cytoprotective role during the basal apoptotic program in hemocytes 30 , which may be an important antiviral mechanism. As for LvΔNp53, its interaction with NF-κB could induce the expression of WSSV IEs, which is propitious to WSSV infection.…”

Section: Discussionmentioning

confidence: 99%

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Identification of two p53 isoforms from Litopenaeus vannamei and their interaction with NF-κB to induce distinct immune response

Wang

Chen

et al. 2017

Sci Rep

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p53 is a transcription factor with capability of regulating diverse NF-κB dependent biological progresses such as inflammation and host defense, but the actual mechanism remains unrevealed. Herein, we firstly identified two novel alternatively spliced isoforms of p53 from Litopenaeus vannamei (LvΔNp53 and the full-length of p53, LvFLp53). We then established that the two p53 isoforms exerted opposite effects on regulating NF-κB induced antimicrobial peptides (AMPs) and white spot syndrome virus (WSSV) immediate-early (IE) genes expression, suggesting there could be a crosstalk between p53 and NF-κB pathways. Of note, both of the two p53 isoforms could interact directly with LvDorsal, a shrimp homolog of NF-κB. In addition, the activation of NF-κB mediated by LvDorsal was provoked by LvΔNp53 but suppressed by LvFLp53, and the increased NF-κB activity conferred by LvΔNp53 can be attenuated by LvFLp53. Furthermore, silencing of LvFLp53 in shrimp caused higher mortalities and virus loads under WSSV infection, whereas LvΔNp53-knockdown shrimps exhibited an opposed RNAi phenotype. Taken together, these findings present here provided some novel insight into different roles of shrimp p53 isoforms in immune response, and some information for us to understand the regulatory crosstalk between p53 pathway and NF-κB pathway in invertebrates.

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“…Shrimp is a highly valued product for its pleasant taste and has a high demand from consumers. In addition, it has advantageous characteristics for aquaculture including high growth performance, high survival rate and disease resistance (Cuzon et al 2004); this organism can tolerate a wide range of physicochemical parameters such as temperature, salinity and dissolved oxygen (Moss et al 2007; Félix‐Portillo et al 2016; Kim et al 2017b). Therefore, taking advantage of these features crop intensification has been stimulated (Cuzon et al 2004; Moss et al 2007; Félix‐Portillo et al 2016; Kim et al 2017b; Thakur et al 2018).…”

Section: Most Important Bft‐based Cultures: Shrimp and Tilapiamentioning

confidence: 99%

The nitrification process for nitrogen removal in biofloc system aquaculture

Robles‐Porchas

Gollas‐Galván

Martínez‐Porchas

et al. 2020

Reviews in Aquaculture

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Aquaculture is an economic activity that faces the unavoidable problem of water quality detriment, which is mainly generated by the improper management of production ponds, including inadequate water circulation and aeration, accumulation of undigested food residues, excretion of metabolic by‐products by the cultivated organisms and other. In addition, the increase in suspended organic matter together with the presence and generation of nitrogen compounds can severely affect the physiology of the animal, leading to significant losses in production. Ammonia (NH3), nitrite (NO2) and nitrate (NO3) are toxic in different scales and environmental conditions; therefore, reducing their concentrations in the culture units has paramount relevance. In this regard, bioflocs are an efficient alternative to transform nitrogen compounds into a non‐toxic form, taking advantage of the capabilities of the microbial communities conforming them. Also, nitrogen can be harnessed and incorporated as organic nitrogen, making the biofloc a source of useful natural food for the cultivated organism. The substantial reduction in the rate of water exchange favoured by the biofloc technology (BFT) is a beneficial advantage for both the production systems and the environment, diminishing the risk of introducing pathogens into the pond in parallel with the improvement in the water quality of effluents. Ammonia oxidation is an advantage adding value to the BFT systems and is discussed in this review.

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Hypoxia drives apoptosis independently of p53 and metallothionein transcript levels in hemocytes of the whiteleg shrimp Litopenaeus vannamei

Cited by 40 publications

References 67 publications

Hypoxia-induced miR-92a regulates p53 signalling pathway and apoptosis by targeting calcium-sensing receptor in Genetically Improved Farmed Tilapia (Oreochromis niloticus)

Hypoxia-induced miR-92a regulates p53 signalling pathway and apoptosis by targeting calcium-sensing receptor in Genetically Improved Farmed Tilapia (Oreochromis niloticus)

Identification of two p53 isoforms from Litopenaeus vannamei and their interaction with NF-κB to induce distinct immune response

The nitrification process for nitrogen removal in biofloc system aquaculture

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