2021
DOI: 10.1016/j.scr.2021.102317
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Hypoxia depletes contaminating CD45+ hematopoietic cells from murine bone marrow stromal cell (BMSC) cultures: Methods for BMSC culture purification

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Cited by 5 publications
(4 citation statements)
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“…The Colony Forming Unit – Fibroblastic (CFU-F) assay was performed as published, with the exception of placing cells in hypoxia based on a recent publication demonstrating reduction of contaminating cells ( Guo et al, 2020 ; Robey et al, 2014 ). In short, cells were isolated from bisected tibia and femur of 2-month-old Col10a1Cre; R26-tdTomato mice centrifuged at 4°C twice pulsed up to 5 K xG.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The Colony Forming Unit – Fibroblastic (CFU-F) assay was performed as published, with the exception of placing cells in hypoxia based on a recent publication demonstrating reduction of contaminating cells ( Guo et al, 2020 ; Robey et al, 2014 ). In short, cells were isolated from bisected tibia and femur of 2-month-old Col10a1Cre; R26-tdTomato mice centrifuged at 4°C twice pulsed up to 5 K xG.…”
Section: Methodsmentioning
confidence: 99%
“…The Colony Forming Unit -Fibroblastic (CFU-F) assay was performed as published, with the exception of placing cells in hypoxia based on a recent publication demonstrating reduction of contaminating cells. (Guo et al, 2021;Robey et al, 2014) In short, cells were isolated from bisected tibia and femur of 2M old Col10a1Cre; R26-tdTomato mice centrifuged at 4C twice pulsed up to 5K xG. Following red blood cell lysis (Roche), cells were plated at 750K cells per 15cm tissue culture plate in complete media (a-MEM, 20% FBS, 1% penicillinstreptomycin) and glutamax (Gibco) after straining through a 70um filter.…”
Section: Colony Forming Unit Assaysmentioning
confidence: 99%
“…[46] As for BMSC isolation, BMSCs were isolated by flushing the bone marrow from the long bones of hind limbs. [47] Then BMSCs were cultured in high glucose DMEM medium supplemented with 20% FBS and 1% penicillin/streptomycin.…”
Section: Methodsmentioning
confidence: 99%
“…The previous evidence indicated that the number of CD13 + CD29 + CD44 + CD73 + CD90 + CD105 + CD151 + CD34 − BMSCs colonies developed was higher under hypoxia (3% O2) [ 92 ]. The recent study of Guo showed that PDGFRα + and LepR + BMSC subpopulations exhibited increased proliferation under 1% O2, while a 10-fold reduction in CD45 + hematopoietic cells [ 93 ]. In addition to proliferation, hypoxia also can influence specific BMSC subpopulations differentiation.…”
Section: Functional Heterogeneity Of Bmsc Subpopulations In Physiolog...mentioning
confidence: 99%