Hypoxia conditioned mesenchymal stem cell-derived extracellular vesicles induce increased in vitro vascular tube formation

Almeria, Ciarra; Weiss, René; Roy, Michelle G.; Tripisciano, Carla; Kasper, Cornelia; Weber, Viktoria; Egger, Dominik

doi:10.1016/s1465324921004618

Cited by 9 publications

(14 citation statements)

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“…In addition, hypoxiaprimed MSCs produced exosomes that attenuated inflammation and elevated fat survival after grafting [136] with upregulated levels of angiopoietin-1, epidermal growth factor (EGF), and fibroblast growth factor proteins [134]. Vesicles produced by hypoxia-conditioned MSCs also induced protein kinase A signaling, vascular endothelial growth factor expression, and thus their angiogenic potential in vitro [137].…”

Section: Hypoxic Preconditioning Of Mscsmentioning

confidence: 99%

New Approaches for Enhancement of the Efficacy of Mesenchymal Stem Cell-Derived Exosomes in Cardiovascular Diseases

Ahmed

Al-Massri

2022

Tissue Eng Regen Med

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Cardiovascular diseases (CVDs) remain a major health concern worldwide, where mesenchymal stem cells (MSCs) therapy gives great promise in their management through their regenerative and paracrine actions. In recent years, many studies have shifted from the use of transplanted stem cells to their secreted exosomes for the management of various CVDs and cardiovascular-related diseases including atherosclerosis, stroke, myocardial infarction, heart failure, peripheral arterial diseases, and pulmonary hypertension. In different models, MSC-derived exosomes have shown beneficial outcomes similar to cell therapy concerning regenerative and neovascular actions in addition to their anti-apoptotic, anti-remodeling, and anti-inflammatory actions. Compared with their parent cells, exosomes have also demonstrated several advantages, including lower immunogenicity and no risk of tumor formation. However, the maintenance of stability and efficacy of exosomes after in vivo transplantation is still a major concern in their clinical application. Recently, new approaches have been developed to enhance their efficacy and stability including their preconditioning before transplantation, use of genetically modified MSC-derived exosomes, or their utilization as a targeted drug delivery system. Herein, we summarized the use of MSC-derived exosomes as therapies in different CVDs in addition to recent advances for the enhancement of their efficacy in these conditions. Graphical abstract

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Section: Hypoxic Preconditioning Of Mscsmentioning

confidence: 99%

New Approaches for Enhancement of the Efficacy of Mesenchymal Stem Cell-Derived Exosomes in Cardiovascular Diseases

Ahmed

Al-Massri

2022

Tissue Eng Regen Med

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“…36,37 The 3D microenvironment promotes proliferation and cellular signaling and enhances the secretion of angiogenic EVs and antiinflammatory factors. 38 According to recent research, MSCs cultured in collagen hydrogel not only caused osteogenic differentiation, but their EVs also showed more osteogenic potential than 2D grown MSC-EVs. 39 For large-scale EV pro-Fig.…”

Section: D and 3d Cell Culture Approaches Impact Mscs' Bone-regenerat...mentioning

confidence: 99%

“…40,41 Various factors like biophysical cues, cellular reprogramming, hypoxic conditions, priming with active agents, etc., can modulate MSCs and increase the secretion of therapeutically more potent EVs. 38,42,43 To reduce the cost associated with every time starting from primary cells, an alternative method can be used by genetic modifications, and MSCs could be immortalized. This could serve as a continuous source of EVs with minimum batch-to-batch variations.…”

Section: Reviewmentioning

confidence: 99%

Well-orchestrated physico-chemical and biological factors for enhanced secretion of osteogenic and angiogenic extracellular vesicles by mesenchymal stem cells in a 3D culture format

2022

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“…In a study, Sun showed that MSC-Exo with abundant HIF-1a can increase the mRNA and protein levels of proangiogenic factors (e.g., VEGF and PDGF) and enhance neovessel formation to provide cardioprotection [59]. Hypoxic conditions can enhance this function [60]. Wang et al [44] found that these proangiogenic effects were induced by the upregulation of miRNA-221-3p.…”

Section: Msc-exos Promote Angiogenesismentioning

confidence: 99%

Mesenchymal Stem Cell-Derived Exosomes in Cardioprotection: A Novel Application to Prevent Myocardial Injury

Yang¹,

Li²,

Tang³

et al. 2022

Rev. Cardiovasc. Med.

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Perioperative myocardial injury is a common complication caused by major surgery. Many pharmacological and nonpharmacological studies have investigated perioperative cardioprotection. However, the methods are insufficient to meet the increasing clinical needs for cardioprotection. The application of Mesenchymal Stem Cell-Derived Exosomes (MSC-Exos) is a novel cell-free therapeutic strategy and has significantly benefitted patients suffering from various diseases. In this review, we comprehensively analyzed the application of MSC-Exos to prevent myocardial infarction/injury by regulating inflammatory reactions, inhibiting cardiomyocyte apoptosis and autophagy, promoting angiogenesis, and mediating cardiac remodeling. Finally, we assessed the therapeutic effects and the challenges associated with the application of MSC-Exos from a clinical perspective.

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Hypoxia conditioned mesenchymal stem cell-derived extracellular vesicles induce increased in vitro vascular tube formation

Cited by 9 publications

References 0 publications

New Approaches for Enhancement of the Efficacy of Mesenchymal Stem Cell-Derived Exosomes in Cardiovascular Diseases

New Approaches for Enhancement of the Efficacy of Mesenchymal Stem Cell-Derived Exosomes in Cardiovascular Diseases

Well-orchestrated physico-chemical and biological factors for enhanced secretion of osteogenic and angiogenic extracellular vesicles by mesenchymal stem cells in a 3D culture format

Mesenchymal Stem Cell-Derived Exosomes in Cardioprotection: A Novel Application to Prevent Myocardial Injury

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