Hypoxia and retinoic acid-inducible NDRG1 expression is responsible for doxorubicin and retinoic acid resistance in hepatocellular carcinoma cells

Jung, Eunjin; Yoon, Jeesun; Lee, Youn Jae; Lee, Jeong-Hoon; Kim, Bo Hyun; Yu, Su Jong; Myung, Sun Jung; Kim, Yoon Jun; Lee, Hyo Suk

doi:10.1016/j.canlet.2010.05.020

Cited by 48 publications

(35 citation statements)

References 26 publications

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“…Some of the differential proteins were reported to be related to cancer. For example, NDRG1 was detected to be over-expressed in malignant lesions (classical pattern and follicular variant of papillary thyroid carcinomas, follicular carcinomas, and metastases of thyroid carcinomas) compared to benign thyroid lesions (goiter and follicular adenomas) [19][20][21][22][23]. In this work, we identified NDRG1 in two protein spots both upregulated in the MG-63 cell.…”

Section: Discussionmentioning

confidence: 83%

“…NDRG1, HLA-B27, and ATCB have PM locations, while NDUFS3 and CALR were reported not to be located in PM, which might be because they are proteins with multiple locations. Given the fact that NDRG1 is closely related to hypoxia-induced chemoresistance, it is highly worth further studying [19][20][21][22]. According to their functions as shown in Table 1, the five differentially expressed proteins can be classified into two groups.…”

Section: Discussionmentioning

confidence: 99%

“…19), normal precursor of osteosarcoma, were used as the comparative model. Seven differential protein spots corresponding to five non-redundant proteins were identified by 2DE-MS strategy, including ACTB, NDRG1, HLA-B27, NDUFS3, and CALR.…”

Section: Discussionmentioning

confidence: 99%

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Plasma membrane proteomic analysis of human osteosarcoma and osteoblastic cells: revealing NDRG1 as a marker for osteosarcoma

et al. 2011

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Osteosarcoma (OS) is the most common primary malignant bone tumor in children and adolescents. To identify new biomarkers for early diagnosis of OS and novel therapeutic candidates, we carried out a plasma membrane proteomic study based on two-dimensional electrophoresis (2DE). The OS cell line MG-63 and the human osteoblastic cell line hFOB1.19 were adopted as the comparison model. We extracted plasma membrane by aqueous two-phase partition extraction. The proteins were separated through 2DE. We analyzed the differentially expressed proteins by Imagemaster software and then identified them by liquid chromatography-tandem mass spectrometry, and the location and function of differential proteins were searched through the Gene Ontology database. In total, 220 protein spots were separated by 2DE. Seven proteins with more than 2.0-folds of difference were successfully identified from 13 gel spots, with 6 up-regulated and 1 down-regulated. Gene Ontology analysis of the differentially expressed proteins indicated that these proteins were involved in seven kinds of functions including binding, structural, cell motility, receptor activity, electron carrier activity, NADH dehydrogenase (ubiquinone) activity, and transcription repressor activity. The up-regulation of NDRG1 was verified in osteosarcoma through Western blotting and by immunohistochemistry in paraffin-embedded tissues. The plasma membrane proteins identified in this study may provide new insights into osteosarcoma cancer biology and potential diagnostic and therapeutic biomarkers.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

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Plasma membrane proteomic analysis of human osteosarcoma and osteoblastic cells: revealing NDRG1 as a marker for osteosarcoma

et al. 2011

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“…Caspase-related markers of apoptosis such as CFLAR and ESPL1, the cell-death related markers NGFRAP1 (also called ''death receptor''), EDARADD, and PDCD1 were overexpressed. We also noticed the down-modulation of proapoptotic signals, including NDRG1 [25], WT1 [26], and YWHAZ [27], as well as the up-regulation of antiapoptotic genes (BIRC5, GADD45B, and MAPK family).…”

Section: Dna Microarray Analysismentioning

confidence: 92%

Toxicity of Doxorubicin on Pig Liver After Chemoembolization with Doxorubicin-loaded Microspheres: A Pilot DNA-microarrays and Histology Study

Verret¹,

Namur²,

Ghegediban³

et al. 2012

Cardiovasc Intervent Radiol

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“…From in vitro studies, it has come forward that there is a relationship between mTOR inhibition and hypoxia [66]. Hypoxia can also elicit multidrug resistance to chemotherapeutics [67] and protect cells from drug-induced apoptosis [68][69][70]. Several studies have found a link between hypoxia and oxidative stress, one of the factors strongly associated with the development of cirrhosis and HCC and therefore an attractive candidate for antitumor therapy [71][72][73].…”

Section: Hypoxia and Hccmentioning

confidence: 99%

Chronic Hypoxia Emerging as One of the Driving Forces behind Gene Expression and Prognosis of Hepatocellular Carcinoma

et al. 2011

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Hepatocellular carcinoma (HCC) is one of the most frequent malignant tumors and an important cause of death. It has become evident that the tumor microenvironment, including hypoxia, plays a major role in the development of HCC. This paper focuses on the role of chronic hypoxia in HCC. Recently, we have shown the importance of chronic hypoxia on gene expression and behavior of liver cells. Using a cell culture model, we identified a distinct gene expression pattern and demonstrated clinical relevance for a 7-gene subset that can predict survival and early recurrence in patients. Recently, it was also shown that chronic hypoxia is associated with the upregulation of β-catenin and Hif1α and that suppression of β-catenin reduced the metastatic potential of the tumor. Both studies demonstrate the importance of chronic hypoxia for the prognosis of HCC. Identifying the molecular pathways can help us to understand the mechanisms responsible for tumor aggressiveness.

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Hypoxia and retinoic acid-inducible NDRG1 expression is responsible for doxorubicin and retinoic acid resistance in hepatocellular carcinoma cells

Cited by 48 publications

References 26 publications

Plasma membrane proteomic analysis of human osteosarcoma and osteoblastic cells: revealing NDRG1 as a marker for osteosarcoma

Plasma membrane proteomic analysis of human osteosarcoma and osteoblastic cells: revealing NDRG1 as a marker for osteosarcoma

Toxicity of Doxorubicin on Pig Liver After Chemoembolization with Doxorubicin-loaded Microspheres: A Pilot DNA-microarrays and Histology Study

Chronic Hypoxia Emerging as One of the Driving Forces behind Gene Expression and Prognosis of Hepatocellular Carcinoma

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