Hypoxia and metallothionein expression in squamous cell carcinomas of the cervix or head and neck using pimonidazole as a marker of tumor hypoxia at the cellular level

Raleigh, James A.; Chou, Shu-Chuan; Rinker, Lillian H.; Weissler, Mark C.; Fowler, Wesley C.; Novotny, Debra B.; Varia, Mahesh A.

doi:10.1016/s0360-3016(98)80315-5

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“…Microscopically, tumor hypoxia can be detected by immunohistochemistry staining of biopsies or tumor slices (Raleigh et al 1998), where hypoxic cells are visualized using exogenous bio-reductive markers such as pimonidazole or EF5. Immunohistochemistry staining has been used as a physiological reference to assess hypoxia tracer uptake in autoradiography and the results of kinetic modeling in preclinical studies (Cho et al 2009, Troost et al 2008.…”

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confidence: 99%

Preclinical evaluation of parametric image reconstruction of [¹⁸F]FMISO PET: correlation withex vivoimmunohistochemistry

et al. 2013

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Compared to indirect methods, direct parametric image reconstruction (PIR) has the advantage of high quality and low statistical errors. However, it is not yet clear if this improvement in quality is beneficial for physiological quantification. This study aimed to evaluate direct PIR for the quantification of tumor hypoxia using the hypoxic fraction (HF) assessed from immunohistological data as a physiological reference. Sixteen mice with xenografted human squamous cell carcinomas were scanned with dynamic [18F]FMISO PET. Afterward, tumors were sliced and stained with H&E and the hypoxia marker pimonidazole. The hypoxic signal was segmented using k-means clustering and HF was specified as the ratio of the hypoxic area over the viable tumor area. The parametric Patlak slope images were obtained by indirect voxel-wise modeling on reconstructed images using filtered back projection and ordered-subset expectation maximization (OSEM) and by direct PIR (e.g., parametric-OSEM, POSEM). The mean and maximum Patlak slopes of the tumor area were investigated and compared with HF. POSEM resulted in generally higher correlations between slope and HF among the investigated methods. A strategy for the delineation of the hypoxic tumor volume based on thresholding parametric images at half maximum of the slope is recommended based on the results of this study.

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Section: Introductionmentioning

confidence: 99%

Preclinical evaluation of parametric image reconstruction of [¹⁸F]FMISO PET: correlation withex vivoimmunohistochemistry

et al. 2013

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Hypoxia and metallothionein expression in squamous cell carcinomas of the cervix or head and neck using pimonidazole as a marker of tumor hypoxia at the cellular level

Cited by 1 publication

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Preclinical evaluation of parametric image reconstruction of [¹⁸F]FMISO PET: correlation withex vivoimmunohistochemistry

Preclinical evaluation of parametric image reconstruction of [¹⁸F]FMISO PET: correlation withex vivoimmunohistochemistry

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Hypoxia and metallothionein expression in squamous cell carcinomas of the cervix or head and neck using pimonidazole as a marker of tumor hypoxia at the cellular level

Cited by 1 publication

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Preclinical evaluation of parametric image reconstruction of [18F]FMISO PET: correlation withex vivoimmunohistochemistry

Preclinical evaluation of parametric image reconstruction of [18F]FMISO PET: correlation withex vivoimmunohistochemistry

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Preclinical evaluation of parametric image reconstruction of [¹⁸F]FMISO PET: correlation withex vivoimmunohistochemistry

Preclinical evaluation of parametric image reconstruction of [¹⁸F]FMISO PET: correlation withex vivoimmunohistochemistry