Sensory and non-sensory assessment of frozen fish: Sardinella species and Micromesistius poutassou obtained from four Agricultural Development Programme (ADP) zones (Ibadan/Ibarapa, Ogbomoso, Oyo and Saki) of Oyo State were carried out. Overall acceptability was based on 7-point Hedonic scale (very much liked (7)-very much disliked (1). Chemical tests [Hypoxanthine (Hx), Peroxide value (PV), Trimethylamine (TMA) and Free fatty acid (FFA)] were carried out using standard methods. Data were analyzed using the mean, standard deviation and t-test.Overall acceptability of the two frozen fish species was between 5.47±0.20 and 5.90±0.08.Hx values varied between 22.15±1.04 and 25.96±0.53mg/100g fish for Sardinella spp. and M. poutassou respectively. The values recorded for both Sardinella spp. and M. poutassou were between 17.48±0.81 and 19.79±1.03 meq/kg for PV; 19.03±065 and 23.63±0.60 mg/100g fish for TMA and between 1.38±0.07% and 1.85±0.31% for FFA respectively. In both Sardinella spp. and M. Poutassou respectively, Oyo Zone had the least PV (17.48±0.81; 17.90±0.60meq/kg), TMA level (20.58±0.90; 19.03±0.65mg/100g). Sardinella spp. samples from the Ogbomoso Zone had highest PV (18.83±0.60meq/kg), FFA (1.85±0.13%) and TMA (23.63±0.60mg/100g) levels, and also the highest FFA values (1.81±0.19%) in M. poutassou. Hypoxanthine and TMA valueswere significantly different in the Sardinella spp. and M. poutassou samples across the zones, respectively.Quality of frozen fish was best in the Oyo Zone.All sensory and non-sensory indicators of fish quality were however within acceptable limits in the four zones of Oyo State.