Hypouricemic, hepatoprotective and nephroprotective roles of oligopeptides derived from <i>Auxis thazard</i> protein in hyperuricemic mice

WEI, Liuyi; Ji, Hongwu; Song, Wenkui; Peng, Shuo; Zhan, Suhong; Qu, Yushan; Chen, Ming; Zhang, Di; Liu, Shucheng

doi:10.1039/d1fo02539b

Cited by 15 publications

(20 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Specifically, THLPK was shown to have the worst effects on decreasing MDA content, while the effect of LPK was the best ( P < 0.05). Similarly, the oligopeptides derived from Auxis thazard were proved to lower MDA content in the liver . Moreover, the inhibited activity of LDH causes cells to produce ATP through oxidative phosphorylation, which upregulates ROS levels in mitochondria .…”

Section: Resultsmentioning

confidence: 95%

“…Similarly, the oligopeptides derived from Auxis thazard were proved to lower MDA content in the liver. 65 Moreover, the inhibited activity of LDH causes cells to produce ATP through oxidative phosphorylation, which upregulates ROS levels in mitochondria. 66 Compared with the IR-model group, the activity of LDH was significantly inhibited by the oligopeptides (P < 0.05).…”

Section: Oligopeptides Restore the Abnormal Expression And Translocat...mentioning

confidence: 99%

See 1 more Smart Citation

Assessment of Novel Oligopeptides from Rapeseed Napin (Brassica napus) in Protecting HepG2 Cells from Insulin Resistance and Oxidative Stress

Yao

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

Oligopeptides (Thr–His–Leu–Pro–Lys (THLPK), His–Pro–Leu–Lys (HPLK), Leu–Pro–Lys (LPK), His–Leu–Lys (HLK), and Leu–His–Lys (LHK)) are newly identified from rapeseed napin (Brassica napus) protein-derived hydrolysates with the capability of upregulating glucose transporter-4 (GLUT4) expression and translocation. However, whether each of them enhances GLUT4 expression and translocation and their specific mechanisms remain unclear. Here, we assess the effects of the oligopeptides against insulin resistance (IR) and oxidative stress in hepatocytes and screen out the most antidiabetic one. Specifically, compared with other oligopeptides, LPK not only remarkably elevated glucose consumption to 8.45 mmol/L protein; superoxide dismutase (SOD) activity to 319 U/mg protein; GLUT4 expression and translocation; and phosphorylated level of insulin receptor substrate-1 (IRS-1), phosphatidylinositol 3-kinase (PI3K), and protein kinase B (Akt) (P < 0.05) but also remarkably attenuated the reactive oxygen species (ROS) level to 2255, lactate dehydrogenase (LDH) activity to 20.5 U/mg protein, malondialdehyde (MDA) content to 241 nmol/mg protein, and NO content to 1302 μmol/mL protein (P < 0.05). These findings demonstrated that antidiabetic oligopeptide LPK possessed the most potential to protect HepG2 cells from IR and oxidative stress via activating IRS-1/PI3K/Akt/GLUT4 and regulating common oxidative markers in vitro.

show abstract

Section: Resultsmentioning

confidence: 95%

Section: Oligopeptides Restore the Abnormal Expression And Translocat...mentioning

confidence: 99%

Assessment of Novel Oligopeptides from Rapeseed Napin (Brassica napus) in Protecting HepG2 Cells from Insulin Resistance and Oxidative Stress

Yao

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…As shown in Figure A, molecular weight <1, 1–2, 2–3, and 3–5 kDa in TAP accounted for 91.40, 7.03, 1.11, and 0.46%, respectively, suggesting that TAP may have potential activity. Small molecular weight (1 kDa) oligopeptides tend to exhibit bioactivity and are more easily absorbed by the body . At present, the molecular weight of most identified XOD inhibitory peptides and/or antihyperuricemic peptides is below 1000 Da, such as Ala-Glu-Ala-Gln-Met-Trp-Arg, Ala-Leu-Pro-Met, Leu-Trp-Met, Pro-Glu-Trp, Ala-Leu-Ser-Gly-Ser-Trp, Gly-Gly-Tyr-Gly-Ile-Phe, Met-Ala-Ile-Gly-Leu-Trp, Ala-Cys-Glu-Cys-Asp .…”

Section: Resultsmentioning

confidence: 99%

Identification of Hyperuricemia Alleviating Peptides from Yellow Tuna Thunnus albacares

Hao,

Ding,

Fan

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

The development of food-derived antihyperuricemic substances is important for alleviating hyperuricemia (HUA) and associated inflammation. Here, novel peptides fromThunnus albacares (TAP) with strong antihyperuricemic activity were prepared. TAP was prepared by alkaline protease (molecular weight <1000 Da), with an IC 50 value of xanthine oxidase inhibitory activity of 2.498 mg/mL, and 5 mg/mL TAP could reduce uric acid (UA) by 33.62% in human kidney-2 (HK-2) cells (P < 0.01). Mice were fed a high-purine diet and injected with potassium oxonate to induce HUA. Oral administration of TAP (600 mg/kg/d) reduced serum UA significantly by 42.22% and increased urine UA by 79.02% (P < 0.01) via regulating urate transporters GLUT9, organic anion transporter 1, and ATP-binding cassette subfamily G2. Meantime, TAP exhibited hepatoprotective and nephroprotective effects, according to histological analysis. Besides, HUA mice treated with TAP showed anti-inflammatory activity by decreasing the levels of toll-like receptor 4, nuclear factors-κB p65, NLRP3, ASC, and Caspase-1 in the kidneys (P < 0.01). According to serum non-targeted metabolomics, 91 differential metabolites between the MC and TAP groups were identified, and purine metabolism was considered to be the main pathway for TAP alleviating HUA. In a word, TAP exhibited strong antihyperuricemic activity both in vitro and in vivo.

show abstract

“…Hyperuricemia is characterized by high levels of UA in serum [ 37 ], and previous studies have shown that some small molecular weight peptides (<1 kDa) could lower the serum UA level [ 18 , 38 , 39 ]. In this study, SISH was confirmed to contain a large number of small peptides (up to 90.7%, <1 kDa) and shown to reduce serum UA levels in hyperuricemic rats, effects that were validated in serum metabolome analysis, as well.…”

Section: Discussionmentioning

confidence: 99%

Sacha Inchi Oil Press-Cake Protein Hydrolysates Exhibit Anti-Hyperuricemic Activity via Attenuating Renal Damage and Regulating Gut Microbiota

Wang

et al. 2022

Foods

View full text Add to dashboard Cite

The incidence of hyperuricemia has increased globally due to changes in dietary habits. The sacha inchi oil press-cake is generally discarded, resulting in the waste of resources and adverse environmental impact. For the purpose of developing sacha inchi oil press-cake and identifying natural components with anti-hyperuricemic activities, we systemically investigated the underlying mechanisms of sacha inchi oil press-cake protein hydrolysates (SISH) in the hyperuricemic rat model. SISH was obtained from sacha inchi oil press-cake proteins after trypsin treatment, and 24 peptides with small molecular weight (<1000 Da) were identified. The results of animal experiments showed that SISH significantly decreased the serum uric acid (UA) level by inhibiting the xanthine oxidase (XOD) activity and regulating the gene expression related to UA production and catabolism in hyperuricemia rats, such as Xdh and Hsh. In addition, SISH attenuated the renal damage and reduced the gene expression related to inflammation (Tlr4, Map3k8, Pik3cg, Pik3ap1, Ikbke, and Nlrp3), especially Tlr4, which has been considered a receptor of UA. Notably, SISH reversed high purine-induced gut microbiota dysbiosis, particularly by enhancing the relative abundance of butyric acid-producing bacteria (unidentified_Ruminococcaceae, Oscillibacter, Ruminiclostridium, Intestinimonas). This research provided new insights into the treatment of hyperuricemia.

show abstract

Hypouricemic, hepatoprotective and nephroprotective roles of oligopeptides derived from Auxis thazard protein in hyperuricemic mice

Abstract: The oligopeptides derived from Auxis thazard protein (ATO) are a class of small peptides with molecular weight <1 kDa and good bioactivity.

Cited by 15 publications

References 51 publications

Assessment of Novel Oligopeptides from Rapeseed Napin (Brassica napus) in Protecting HepG2 Cells from Insulin Resistance and Oxidative Stress

Assessment of Novel Oligopeptides from Rapeseed Napin (Brassica napus) in Protecting HepG2 Cells from Insulin Resistance and Oxidative Stress

Identification of Hyperuricemia Alleviating Peptides from Yellow Tuna Thunnus albacares

Sacha Inchi Oil Press-Cake Protein Hydrolysates Exhibit Anti-Hyperuricemic Activity via Attenuating Renal Damage and Regulating Gut Microbiota

Contact Info

Product

Resources

About