Hypouricemic and Nephroprotective Effects of an Active Fraction from <b><i>Polyrhachis Vicina Roger</i></b> On Potassium Oxonate-Induced Hyperuricemia in Rats

Abstract

Background/Aims: The objective of this study is to evaluate the hypouricemic and nephroprotective effects of an active fraction from Polyrhachis vicina Roger (AFPR) in potassium oxonate-induced hyperuricemic rats. Methods: Hyperuricemia was induced by potassium oxonate in male rats. AFPR was orally administered to hyperuricemic rats for 12 consecutive weeks. Serum, liver and kidney samples were collected for effects and mechanism analysis. The levels of serum uric acid (SUA) were measured by the phosphotungstic acid method, xanthine oxidase (XOD) activity in the hepatic and serum samples were measured by ultraviolet spectrophotometry, serum levels of interleukin-1 (IL-1β), interleukin-1 (IL-6) and tumor necrosis factor-α (TNF-α) were measured by ELISA, the levels of serum creatinine (SCr), blood urea nitrogen (BUN), super oxide dismutase (SOD) and malondialdehyde (MDA) in serum were determined by colorimetric method. Protein expression of renal URAT1, GLUT9, and OAT1 were analyzed by Western blot. Results: AFPR significantly decreased the levels of SUA, serum and hepatic XOD, SCr, BUN, and MDA as well as increased SOD. In addition, AFPR treatment significantly reduced the levels of proinflammatory cytokines in serum, including IL-1β, IL-6 and TNF-α. Moreover, we found the significant decrease in protein expression of URAT1 and GLUT9, and the significant increase in protein expression of OAT1 in the kidney in AFPR treated groups compared to the model groups of hyperuricemia. Conclusion: These findings suggest that AFPR has anti-hyperuricemic activity attributed to the inhibition of uric acid generation in the liver and probably to the enhancement of urate excretion in the kidney, and possess nephroprotective effect in hyperuricemic rats due to its anti-inflammatory and antioxidant activities.

Characterization, Antioxidant Activities, and Pancreatic Lipase Inhibitory Effect of Extract From the Edible Insect Polyrhachis vicina

“…The ratio of omega-6/omega-3 was less than 5 in other edible insects, such as Clanis bilineata . This ratio meets the health requirements of human intake ( 13 ). An appropriate proportion of omega-6/omega-3 fatty acids exerts various bioactivities, including anticancer, anti-atherosclerotic, antithrombotic, anti-inflammatory, and antidiabetes activities, in humans ( 33 ).…”

“…However, limited studies have examined the phenolic components of P. vicina . Previous studies have reported that P. vicina extracts exert hyperuricemia and exhibit anti-inflammatory and antitumor activities ( 12 , 13 ). Thus, the practice of P. vicina supplementation in wine has continued for many years in China or certain Western countries.…”

Synergistic Impacts of Alpinia oxyphylla Seed Extract and Allopurinol against Experimental Hyperuricemia

“…It was reported that there was a close correlation between serum urate and creatinine concentrations in gout patients [ 23 ]. Increases in serum BUN and creatinine indicate nephrotoxicity and cause renal damage in hyperuricemia [ 24 ]. Our results suggested that the addition of AE to AP therapy could prevent renal damage.…”

Withaferin A protects against hyperuricemia induced kidney injury and its possible mechanisms

“…PO is most frequently used to set up animal models of hyperuricemia via suppressing uricase that converts UA to allantoin [ 22 ]. In this study, we successfully established PO-induced hyperuricemic mouse model, and evaluated the protective effect of WFA.…”