Hypotonic stress induces translocation of the osmolyte channel protein pICln in embryonic skate (Raja eglanteria) heart

Musch, Mark W.; Luer, Carl A.; Davis-Amaral, Erin M.; Goldstein, Léon

doi:10.1002/(sici)1097-010x(19970415)277:6<460::aid-jez6>3.0.co;2-m

Cited by 30 publications

(13 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, transferring the cells into a hypotonic solution shifts a significant amount (approximately 50%) of the ICln protein to the membrane fraction. Moreover, returning the cells to the isotonic medium shifts the ICln protein back to the cytosolic fraction (Musch et al 1997(Musch et al , 1998. The duration of the volume stress appears to be crucial, since exposure of the cells to a hypotonic medium for only a few minutes does not result in a significant redistribution of ICln in C6 glioma and EAhy926 endothelial cells (Buyse et al 1997;Emma et al 1998a).…”

Section: Introductionsupporting

confidence: 63%

Icln, An Ion Channel‐Forming Protein Associated with Cell Volume Regulation

Gschwentner

Fürst

Ritter

et al. 1999

Experimental Physiology

View full text Add to dashboard Cite

It is not resolved whether the anionic channel involved in volume regulation after cell swelling comprises one or more subunits. Moreover, it remains to be determined which of the different proteins cloned so far, for which an involvement in cell volume regulation has been postulated, is the ideal candidate. In this review, we consider the role of the ICln protein, cloned from MDCK cells, in cell volume regulation.

show abstract

Section: Introductionsupporting

confidence: 63%

Icln, An Ion Channel‐Forming Protein Associated with Cell Volume Regulation

Gschwentner

Fürst

Ritter

et al. 1999

Experimental Physiology

View full text Add to dashboard Cite

show abstract

“…3). Similar results were reported for rat neonatal cardiac myocytes in which ICln transiently translocates to the cell membrane, a reversible process that is paralleled by a hypotonicity-stimulated taurine efflux (40,41). In C6 glioma cells, ϳ90% of ICln was found to be located in the cytosol.…”

Section: Discussionmentioning

confidence: 99%

Cell Swelling Stimulates Cytosol to Membrane Transposition of ICln

Ritter

Ravasio

Jakab

et al. 2003

Journal of Biological Chemistry

View full text Add to dashboard Cite

ICln is a multifunctional protein that is essential for cell volume regulation. It can be found in the cytosol and is associated with the cell membrane. Besides its role in the splicing process, ICln is critically involved in the generation of ion currents activated during regulatory volume decrease after cell swelling (RVDC). If reconstituted in artificial bilayers, ICln can form ion channels with biophysical properties related to RVDC. We investigated (

show abstract

“…In the kidney, ICln is located preferentially in the apical membrane of the proximal tubule and both in the cytosol and the membrane of distal tubules [156,292], indicating that the distribution of ICln depends on the cell type and the physiological status of the cell. Accordingly, in fibroblasts and in skate and neonatal rat heart cells hypotonic stress leads to the transposition of ICln from the cytosol to the plasma membrane [156,197,198]. In C6 glioma cells previously adapted to a hypertonic culture medium and in human non-pigmented ciliary epithelial cells, this effect could not be observed [81, 255] following a moderate reduction of extracellular tonicity.…”

Section: Iclnmentioning

confidence: 90%