Hypothyroidism in Children with Filter Paper TSH of 30 to 50 μU/ml at Initial Screening <i>Implication of the TSH Cut‐off Point for Recalling of Infants at Risk</i>

Léger, Juliane; LeMerrer, Martine; Briard, Marie-Louise; Czernichow, P

doi:10.1111/j.1651-2227.1987.tb10527.x

“…The detection limit of 1 mU/l is quite sufficient, as the levels below 20 mU/l are considered negative in the screening of neonatal hypothyroidism (18). A very low detection limit (0.037 mU/l) was described in an assay of Japanese researchers based on a combination of monoclonal antibody and polyclonal antibody (6).…”

Section: Discussionmentioning

confidence: 99%

Immunoradiometric and Luminescence Immunoenzymometric Assay of Human Thyrotropin from Dried Blood Spots for Screening of Neonatal Hypothyroidism

Jirkalová¹,

Čáp²,

Strakova³

et al. 1996

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Summary:Two monoclonal antibodies to thyrotropin prepared in our laboratory were employed for the development of two-site immunometric assays in two modifications for the estimation of human thyrotropin in dried blood spots designed for the screening of neonatal hypothyroidism. The immunoradiometric assay using the second antibody labelled with I25 I is simple and fast (one incubation step lasting 2 h). The detection limit of 1 mU/1 and the absence of the hook effect up to a concentration of more than 1000mU/l are optimal for neonatal screening; the presence of other glycoprotein hormones does not interfere with the assay. In the luminescence immunoenzymometric assay the second antibody is labelled with peroxidase. In spite of the two-step configuration the method is fast (4.5 h) and enables specific determination of thyrotropin levels in the range of 2.4-1100 mU/1. The conditions and properties of both immunometric assays described are comparable with the time-resolved immunofluorometric assay widely used in Europe.The luminescence immunoenzymometric assay was applied successfully in the screening of 3000 neonates for congenital hypothyroidism.

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Immunoradiometric and luminescence immunoenzymometric assay of human thyrotropin from dried blood spots for screening of neonatal hypothyroidism

Jirkalová

¹

,

Čáp²,

Strakova³

et al. 1997

Early Human Development

1

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Summary:Two monoclonal antibodies to thyrotropin prepared in our laboratory were employed for the development of two-site immunometric assays in two modifications for the estimation of human thyrotropin in dried blood spots designed for the screening of neonatal hypothyroidism. The immunoradiometric assay using the second antibody labelled with I25 I is simple and fast (one incubation step lasting 2 h). The detection limit of 1 mU/1 and the absence of the hook effect up to a concentration of more than 1000mU/l are optimal for neonatal screening; the presence of other glycoprotein hormones does not interfere with the assay. In the luminescence immunoenzymometric assay the second antibody is labelled with peroxidase. In spite of the two-step configuration the method is fast (4.5 h) and enables specific determination of thyrotropin levels in the range of 2.4-1100 mU/1. The conditions and properties of both immunometric assays described are comparable with the time-resolved immunofluorometric assay widely used in Europe.The luminescence immunoenzymometric assay was applied successfully in the screening of 3000 neonates for congenital hypothyroidism.

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Hypothyroïdie centrale de découverte néonatale

Giroux¹,

Metz

²

,

Giroux³

et al. 1997

Archives de Pédiatrie

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Hypothyroidism in Children with Filter Paper TSH of 30 to 50 μU/ml at Initial Screening Implication of the TSH Cut‐off Point for Recalling of Infants at Risk

Cited by 5 publications

References 3 publications

Immunoradiometric and Luminescence Immunoenzymometric Assay of Human Thyrotropin from Dried Blood Spots for Screening of Neonatal Hypothyroidism

Immunoradiometric and Luminescence Immunoenzymometric Assay of Human Thyrotropin from Dried Blood Spots for Screening of Neonatal Hypothyroidism

Immunoradiometric and luminescence immunoenzymometric assay of human thyrotropin from dried blood spots for screening of neonatal hypothyroidism

Hypothyroïdie centrale de découverte néonatale

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