Hypothyroidism impairs somatovisceral reflexes involved in micturition of female rabbits

Sánchez-García, Octavio; López-Juárez, Rhode; Rodríguez-Castelán, Julia; Corona-Quintanilla, Dora Luz; Martı́nez–Gómez, Margarita; Cuevas-Romero, Estela; Castelán, Francisco

doi:10.1002/nau.23594

Cited by 5 publications

(18 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nine urethane‐anesthetized (1.5 g/kg, ip) rabbits were used for the simultaneous records of cystometrograms (CMGs), UP, and electromyogram (EMG). We followed a protocol reported elsewhere 17 . Measured urodynamic variables were the volume of saline that triggers voiding (ThV), the total volume expelled during the voiding (VV), and the remaining volume inside the bladder (RV) as the result of ThV minus VV.…”

Section: Methodsmentioning

confidence: 99%

“…We followed a protocol reported elsewhere. 17 Measured urodynamic variables were the volume of saline that triggers voiding (ThV), the total volume expelled during the voiding (VV), and the remaining volume inside the bladder (RV) as the result of ThV minus VV. We also measured the efficiency of voiding (VE) calculated as (VV/VV+RV) × 100, the pressure that triggers the voiding phase (ThP), the highest pressure observed at voiding (MP), the time from ThP to the basal pressure (VD), and the elapsed time between two voiding phases (ICI) ( Figure S1A).…”

Section: Cystometry Urethral Pressure and Electromyogram Recordingmentioning

confidence: 99%

“…The variables measured of the were the pressure that triggered the rise of the UP (ThUP), the maximal pressure during one micturition cycle (maximum urethral pressure [MUP]), the time from the ThUP to the triggering of the MUP (ThtUPD); the pressure at which the UP returned to the basal line (RUP); the time from the ThUP to the next basal pressure (urethral pressure duration [UPD]); the UPC, as the MUP/MP ratio, as an indicator of the pressure to close the urethra; and the urethral resistance during voiding (UR), as the MP divided by the squared maximum flow ([cmH 2 O]/[mL/s] 2 ), 17 ( Figure S1B).…”

Section: Cystometry Urethral Pressure and Electromyogram Recordingmentioning

confidence: 99%

See 2 more Smart Citations

Lumbosacral ventral root avulsion alters reflex activation of bladder, urethra, and perineal muscles during micturition in female rabbits

Corona-Quintanilla

Acosta‐Ortega

Flores‐Lozada

et al. 2020

Neurourology and Urodynamics

Self Cite

View full text Add to dashboard Cite

Aim To determine the effect of the lumbosacral ventral root avulsion (VRA) on the reflex activation of bladder, urethra, and activation of perineal muscles during micturition in female rabbits. Methods We allocated 14 virgin female rabbits to evaluate, first, the gross anatomy of lumbosacral spinal cord root (n = 5) and, second, to determine the effect of VRA on perineal muscles during micturition (n = 9). We recorded cystometrograms, urethral pressure, and electromyograms of the bulbospongiosus (Bsm) and ischiocavernosus (Ism) muscles before and after the L6‐S2 VRA. Standard variables were measured from each recording and analyzed to identify significant differences (P < .05). Results We found that the L6‐S2 VRA affected directly the bladder and urethral function and reduced the duration and the frequency of the bursting of Ism and Bsm muscles during voiding. The Ism and Bsm showed a phasic activation, of different frequencies, during the voiding phase and the L6‐S2 VRA inhibited the co‐contraction of the Ism and Bsm‐bladder‐urethra. Conclusions The Ism and Bsm are activated at different frequencies to trigger the voiding phase. The L6‐S2 VRA affected the activity pattern of both perineal muscles. These modifications affected the bladder and urethra function. It is possible that the restoration of the activation frequency of perineal muscles contributed for an efficient bladder contraction.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Cystometry Urethral Pressure and Electromyogram Recordingmentioning

confidence: 99%

Section: Cystometry Urethral Pressure and Electromyogram Recordingmentioning

confidence: 99%

See 1 more Smart Citation

Lumbosacral ventral root avulsion alters reflex activation of bladder, urethra, and perineal muscles during micturition in female rabbits

Corona-Quintanilla

Acosta‐Ortega

Flores‐Lozada

et al. 2020

Neurourology and Urodynamics

Self Cite

View full text Add to dashboard Cite

show abstract

“…We recorded cystometrograms using a technique described elsewhere . Briefly, urethane‐anesthetized (1.5 g/kg, ip) rabbits were placed in a dorsal supine position.…”

Section: Methodsmentioning

confidence: 99%

“…We quantified the volumes of saline that triggers voiding (ThV), the total volume expelled during the voiding (VV), and the remaining volume inside the bladder as the result of ThV minus VV (RV). We also measured the efficiency of voiding (VE) calculated as (VV/VV + RV) × 100, the pressure that triggers the voiding phase (ThP), the highest pressure observed at voiding (MP), the time from ThP to the basal pressure (VD), and the elapsed time between two voiding phases (ICI) …”

Section: Methodsmentioning

confidence: 99%

Bladder and urethral dysfunction in multiparous and mature rabbits correlates with abnormal activity of pubococcygeus and bulbospongiosus muscles

Corona-Quintanilla

López-Juárez

Pacheco

et al. 2019

Neurourology and Urodynamics

Self Cite

View full text Add to dashboard Cite

Aims: To evaluate the combined effect of age and multiparity on the micturition reflex, including pelvic floor muscle activation.Methods: Young and mature nulliparous rabbits were compared to young and mature multiparas (n = 6 per group). Cystometrograms and urethral pressure (UP) were performed while simultaneously recording the electromyogram (EMG) activity of the pubococcygeus and bulbospongiosus muscles to establish their functional correlation to urological function.Results: Multiparity and age significantly influence the bladder and UP affecting the voiding efficiency and intercontraction interval. Such interaction also reduced the UP threshold, timing, and duration. Other bladder and urethral variables were predominantly affected only by age. Urodynamic alterations correlated with abnormal patterns or absent EMG activity of the pubococcygeus and bulbospongiosus muscles. Conclusions:The present findings strongly suggest that multiparity and age affects specific pelvic floor muscle reflex activation during micturition, and may contribute to alterations in bladder and urethral function. This data broadens our understanding of the critical role of the appropriate activity of the individual pelvic floor muscles in micturition. K E Y W O R D Sbulbospongiosus, cystometrograms, intraurethral, pubococcygeus, stress urinary incontinence

show abstract

Estrogens influence differentially on the pelvic floor muscles activation at somatovisceral reflexes involved in micturition of rabbits

et al. 2021

Self Cite

View full text Add to dashboard Cite

Objective: To determine the estrogen-dependency of the bladder and urethral function and the coordinated activation of pelvic floor muscles (PFM) during micturition.Methods: We allocated age-matched female rabbits to control, 1-month ovariectomized (OVX), and OVX plus 2-week estradiol benzoate (EB) groups to record cystometry, urethral pressure, and electromyograms of bulbospongiosus (Bsm), and pubococcygeus muscles (Pcm) simultaneously. We also measured serum estradiol levels and myofiber cross-sectional area. We assessed urodynamic and urethral variables, categorized the Bsm-Pcm activation patterns at storage and voiding phases, and obtained the power spectrum density of muscle activation around the voiding phase. We investigated the influence of ovarian hormones, in general, and the contribution of estrogen, particularly on the functions of the bladder, urethra, and PFM. Statistical significance was set at P < 0.05.Results: Ovarian hormones influence the bladder, urethral, and PFM functions. The urodynamics analyses indicated estrogens contribute to voiding duration and, to a lesser extent, to the time between bladder contractions. Urethral pressure at closure (maximal pressure-to-maximal urethral pressure ratio) improved partially (8%, P < 0.05) in the OVX plus 2-week estradiol benzoate compared with OVX, but urethral resistance increased ($1.9-fold, P < 0.05) compared with control rabbits. Our findings support that Pcm activity at voiding is estrogensensitive, albeit EB administration reduced it at storage resume, which relates to high urethral resistance.Conclusions: Ovariectomy impairs bladder and urethral pressures and Bsm and Pcm activation at micturition in anesthetized rabbits. Estrogen administration partially reverts some of these effects and influences Pcm activation.

show abstract

Hypothyroidism impairs somatovisceral reflexes involved in micturition of female rabbits

Abstract: Our findings demonstrate hypothyroidism impairs the bladder and, urethral functions, and reflex activation of Pcm and Bsm affecting the micturition in female rabbits.

Cited by 5 publications

References 26 publications

Lumbosacral ventral root avulsion alters reflex activation of bladder, urethra, and perineal muscles during micturition in female rabbits

Lumbosacral ventral root avulsion alters reflex activation of bladder, urethra, and perineal muscles during micturition in female rabbits

Bladder and urethral dysfunction in multiparous and mature rabbits correlates with abnormal activity of pubococcygeus and bulbospongiosus muscles

Estrogens influence differentially on the pelvic floor muscles activation at somatovisceral reflexes involved in micturition of rabbits

Contact Info

Product

Resources

About