Hypothermic preservation of endothelialized gas‐exchange membranes

Pflaum, Michael; Merhej, Hayan; Peredo, Ariana; De, Adim; Dipresa, Daniele; Wiegmann, Bettina; Wolkers, Willem F.; Haverich, Axel; Korossis, Sotirios

doi:10.1111/aor.13776

Cited by 5 publications

(7 citation statements)

References 14 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Post-oxidation, cells underwent washing with PBS twice and were either fixed for staining using 4% paraformaldehyde (PFA) in PBS or replenished with EGM-2 and cultivated for 24 hours. Human endothelial colony forming cells (ECFCs), kindly provided by Michael Pflaum [16], were used as negative controls for IL-B4 and serum staining and treated similar to the PBS controls.…”

Section: Oxidation Of Pecs With Naio4mentioning

confidence: 99%

Reduction of Xenogeneic Epitopes on Porcine Endothelial Cells by Periodate Oxidation

Thom,

Roters,

Schuemann

et al. 2024

Preprint

View full text Add to dashboard Cite

Background: Patterns of humoral immune responses represent a major hurdle in terms of pig-to-human xenotransplantation approaches. The best-known xenogeneic glycan antigens pre-sent in pigs are the αGal (Galili-antigen) and the non-human sialic acid Neu5Gc. As there are further differences between porcine and human cellular surface glycosylation, a much broader range of glycan epitopes with xeno-reactive relevance can be anticipated. Therefore, we set-out to chemically modify porcine cellular surface glycans in a global approach by applying sodium peri-odate (NaIO4) oxidation. Methods: Porcine endothelial cells were exposed to oxidation with 1 to 5 mM NaIO4 for different time periods at 37°C or 4°C and under static or dynamic conditions. The impact on cellular sur-vival was determined applying live/dead-assays. Oxidation of αGal-epitopes was assessed by flu-orescence microscopy-based quantification of isolectin-B4 (IL-B4) staining. Overall immunogenic-ity of porcine cells was determined by human serum antibody binding. Results: Treatment of porcine endothelial cells and tissues with NaIO4 led to a reduced binding of the αGal-specific IL-B4 and/or human serum antibodies. NaIO4 revealed to be cytotoxic when performed at elevated temperature and for prolonged time. However, by applying 2 mM NaIO4 for 60 min at 4°C a high extent of cellular viability and a relevant reduction of detectable αGal epitope were observed. No differences were detected irrespectively on whether the cells were oxi-dized under static or flow conditions. Conclusion: Glycan epitopes on living cells can be oxidized with NaIO4 while maintaining their viability. Accordingly, this strategy holds promise to prevent immune reactions mediated by pre-formed anti-glycan antibodies.

show abstract

Section: Oxidation Of Pecs With Naio4mentioning

confidence: 99%

Reduction of Xenogeneic Epitopes on Porcine Endothelial Cells by Periodate Oxidation

Thom,

Roters,

Schuemann

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…resulting in reduced gas transfer efficiency, lowered membrane function, and ultimately device failure (80). Other limiting factors relate to platelet and coagulation activation, resulting in clot formation within the system (79).…”

Section: The Sign (+) Is Used As a Scale To Represent Circuit Priming...mentioning

confidence: 99%

“…Despite improved hemocompatibility, the deposition of proteins onto artificial surfaces is still a challenge ( 1 , 24 , 79 ). This includes the formation of a limiting diffusion layer composed of fibrin, single cells, and cell clusters, which can cover large areas of the membrane surface, resulting in reduced gas transfer efficiency, lowered membrane function, and ultimately device failure ( 80 ). Other limiting factors relate to platelet and coagulation activation, resulting in clot formation within the system ( 79 ).…”

Section: Technical Outlookmentioning

confidence: 99%

Trends, Advantages and Disadvantages in Combined Extracorporeal Lung and Kidney Support From a Technical Point of View

Costa

Halfwerk

Wiegmann

et al. 2022

Front. Med. Technol.

Self Cite

View full text Add to dashboard Cite

Extracorporeal membrane oxygenation (ECMO) provides pulmonary and/or cardiac support for critically ill patients. Due to their diseases, they are at high risk of developing acute kidney injury. In that case, continuous renal replacement therapy (CRRT) is applied to provide renal support and fluid management. The ECMO and CRRT circuits can be combined by an integrated or parallel approach. So far, all methods used for combined extracorporeal lung and kidney support present serious drawbacks. This includes not only high risks of circuit related complications such as bleeding, thrombus formation, and hemolysis, but also increase in technical workload and health care costs. In this sense, the development of a novel optimized artificial lung device with integrated renal support could offer important treatment benefits. Therefore, we conducted a review to provide technical background on existing techniques for extracorporeal lung and kidney support and give insight on important aspects to be addressed in the development of this novel highly integrated artificial lung device.

show abstract

“…However, current research on how to establish a single layer of endothelial tissue on the gas exchange of ECLS equipment is not advanced [23]. In addition, the shelf life of an endothelialized oxygenator can, under hypothermic conditions, be stretched up to two weeks [86] compared to the shelf life of an otherwise coated oxygenator being typically 2 years. This would result in complex resource planning and management for both manufacturers and ECLS centers.…”

Section: Endothelializationmentioning

confidence: 99%

New Trends, Advantages and Disadvantages in Anticoagulation and Coating Methods Used in Extracorporeal Life Support Devices

et al. 2021

View full text Add to dashboard Cite

The use of extracorporeal life support (ECLS) devices has significantly increased in the last decades. Despite medical and technological advancements, a main challenge in the ECLS field remains the complex interaction between the human body, blood, and artificial materials. Indeed, blood exposure to artificial surfaces generates an unbalanced activation of the coagulation cascade, leading to hemorrhagic and thrombotic events. Over time, several anticoagulation and coatings methods have been introduced to address this problem. This narrative review summarizes trends, advantages, and disadvantages of anticoagulation and coating methods used in the ECLS field. Evidence was collected through a PubMed search and reference scanning. A group of experts was convened to openly discuss the retrieved references. Clinical practice in ECLS is still based on the large use of unfractionated heparin and, as an alternative in case of contraindications, nafamostat mesilate, bivalirudin, and argatroban. Other anticoagulation methods are under investigation, but none is about to enter the clinical routine. From an engineering point of view, material modifications have focused on commercially available biomimetic and biopassive surfaces and on the development of endothelialized surfaces. Biocompatible and bio-hybrid materials not requiring combined systemic anticoagulation should be the future goal, but intense efforts are still required to fulfill this purpose.

show abstract

Hypothermic preservation of endothelialized gas‐exchange membranes

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 5 publications

References 14 publications

Reduction of Xenogeneic Epitopes on Porcine Endothelial Cells by Periodate Oxidation

Reduction of Xenogeneic Epitopes on Porcine Endothelial Cells by Periodate Oxidation

Trends, Advantages and Disadvantages in Combined Extracorporeal Lung and Kidney Support From a Technical Point of View

New Trends, Advantages and Disadvantages in Anticoagulation and Coating Methods Used in Extracorporeal Life Support Devices

Contact Info

Product

Resources

About