Hypothermia Induced by Adenosine5′-Monophosphate Attenuates Acute Lung Injury Induced by LPS in Rats

Abstract

We have built a rat's model to investigate whether the hypothermia induced by adenosine 5′-monophosphate (5′-AMP) (AIH) could attenuate acute lung injury induced by LPS in rats. We detected the inflammatory cytokine levels in the plasma and bronchoalveolar lavage fluid samples, and we analyzed the pathological changes in the lungs. We have found that AIH can effectively inhibit acute inflammatory reactions and protect the lung from acute injury induced by LPS in rats.

Hypothermia induced by adenosine 5′‐monophosphate attenuates injury in an L‐arginine‐induced acute pancreatitis rat model

“…In addition, many experiments confirmed that hypothermia could reduce pancreatic edema,microvascular permeability and the serum levels of amylase and lipase in acute pancreatitis in rats. Our previous studies showed that 5′‐AMP was able to successfully induce hypothermia and torpor‐like states in rats in a dose‐dependent manner . Here, our studies also revealed that the hypothermia induced by 5′‐AMP could inhibit the early inflammatory response in acute pancreatitis.…”

“…5′‐AMP, together with the appropriate AT, to initiate, maintain, and terminate deep hypometabolism of diverse species of mammals. Miao et al . has confirmed that myocardial ischemia in mice given 5′‐AMP without hypothermia had similar ischemia/reperfusion injuries as the euthermic control.…”

“…The results demonstrated that rats that received the 0.5 g/kg dose entered a state of torpor. Therefore, we utilized a dose of 0.5 g/kg body weight to establish a rat model of hypothermia . 5′‐AMP was dissolved in phosphate‐buffered saline, and the pH of the resulting solution was adjusted to 7.4.…”

Hypothermia in mouse is caused by adenosine A1 and A3 receptor agonists and AMP via three distinct mechanisms

“…Large exogenous doses of AMP (500 to 3500 mg/kg) cause torpor and have protective effects in various injury models (Miao et al , 2015; Miao et al , 2012; Tao et al , 2011; Wang et al , 2014). There is no clearly identified receptor for AMP (although AMP has been proposed to be an A 1 AR ligand (Rittiner et al , 2012)).…”

Adenosine 5′-monophosphate-induced hypothermia inhibits the activation of ERK1/2, JNK, p38 and NF-κB in endotoxemic rats

“…Recently, studies have shown that hypothermia induced by 5′-AMP has an anti-inflammatory effect [10,11,14]. Our previous studies have also shown that hypothermia induced by 5′-AMP could inhibit LPS-induced inflammation [15]. In this study, we sought to elucidate how hypothermia induced by 5′-AMP could act to inhibit inflammatory responses.…”