Hypothalamic expression of serotonin 1A, 2A and 2C receptor and GAD67 mRNA in female cynomolgus monkeys with different sensitivity to stress

Centeno, Maria Luisa; Sanchez, Rachel L.; Cameron, Judy L.; Bethea, Cynthia L.

doi:10.1016/j.brainres.2007.01.056

Cited by 27 publications

(27 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Upon examination of the PVN in hematoxylin-stained sections, it appeared that the PVN was larger and extended further in a caudal direction in the MSR and SS groups (not shown). There was also a significant positive correlation between the expression of CRH mRNA and 5HT2A mRNA [previously reported, [8]].…”

Section: Resultsmentioning

confidence: 54%

“…Bottom: The average CRH mRNA expression in the PVN was significantly correlated with 5HT2A mRNA expression in the PVN. From Centeno et al [8]. …”

Section: Resultsmentioning

confidence: 99%

“…In addition, the average CRH ISH signal was compared to the average signal previously obtained for 5HT2A expression in the PVN [8] in the same animals using a linear regression analysis.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, we observed that SS animals secrete less prolactin in response to a fenfluramine challenge compared to HSR animals [4]; that SS animals have fewer serotonin neurons and lower expression of genes involved in serotonergic neurotransmission compared to HSR animals [5]; that SS animals have higher expression of 5HT2A receptors, 5HT2C receptors and GAD67 in the hypothalamus compared to HSR animals [8], and that SS animals have fewer and smaller GnRH fibers in the median eminence compared to HSR animals [9]. Other regulatory neural systems that are activated in conditions of stress and that impinge upon GnRH neurons include corticotropin-releasing hormone (CRH) and β-endorphin.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Corticotropin-Releasing Hormone and Pro-Opiomelanocortin Gene Expression in Female Monkeys with Differences in Sensitivity to Stress

Centeno

Sanchez

Reddy³

et al. 2007

Neuroendocrinology

Self Cite

View full text Add to dashboard Cite

Background/Aims: The expressions of corticotropin-releasing hormone (CRH) and pro-opiomelanocortin (POMC) were assessed in brain tissue collected from nonstressed female cynomolgus monkeys previously categorized as highly stress resilient (HSR), medium stress resilient (MSR), or stress sensitive (SS) with respect to stress-induced anovulation. Methods: In situ hybridization and quantitative image analysis was used to measure mRNAs coding for CRH in the hypothalamic paraventricular nucleus (PVN) and thalamic center median-subfascicular complex (CM-Sf). Then, CRH neurons in the PVN were immunostained and the area of immunostaining was measured. Also, CRH fibers were immunostained in the central nucleus of the amygdala and the area of immunostaining was obtained. Finally, POMC mRNA expression was characterized in the hypothalamic infundibular nucleus. The groups were compared with ANOVA and Student-Newman-Keul’s (SNK) post hoc comparison. Results: CRH mRNA was significantly elevated in the caudal PVN in the MSR and SS animals compared to HSR animals (p < 0.05, SNK). There was a significant increase in average and total CRH-positive area in the MSR and SS groups compared to the HSR group (p < 0.05, SNK). There was also a significant increase in CRH volume in the MSR and SS groups compared to the HSR group (p < 0.05, SNK). In the CM-Sf, the average CRH optical density was significantly higher in the MSR and SS groups than in the HSR group (p < 0.05, SNK). In the central nucleus of the amygdala, the area of CRH fiber staining was significantly higher in the SS group than in the MSR or HSR groups (p < 0.05, SNK). There was no difference between the groups in POMC mRNA expression in the mediobasal hypothalamus. Conclusion: Macaques that exhibit immediate suppression of reproductive function upon stress are considered stress sensitive. These animals have elevated CRH in the hypothalamus and limbic structures, which may play a role in suppressing the hypothalamic-gonadal axis upon stress initiation.

show abstract

Section: Resultsmentioning

confidence: 54%

“…Bottom: The average CRH mRNA expression in the PVN was significantly correlated with 5HT2A mRNA expression in the PVN. From Centeno et al [8]. …”

Section: Resultsmentioning

confidence: 99%

“…In addition, the average CRH ISH signal was compared to the average signal previously obtained for 5HT2A expression in the PVN [8] in the same animals using a linear regression analysis.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Corticotropin-Releasing Hormone and Pro-Opiomelanocortin Gene Expression in Female Monkeys with Differences in Sensitivity to Stress

Centeno

Sanchez

Reddy³

et al. 2007

Neuroendocrinology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Our study design was limited in our ability to examine these potential mechanisms, as our first goal was to develop a model to test whether synergistic effects of low-level stressors on reproductive function could be demonstrated. More recently however, we have found that female monkeys who readily develop stress-induced reproductive dysfunction when exposed to combined psychosocial and metabolic stresses have elevated levels of anxious behavior (18), depressed expression of a number of genes in the central serotonin pathway as well as increased GABAergic gene expression in the medial basal hypothalamus (12). Our finding that low-level stressors exhibit synergistic effects on the reproductive axis was recently used to guide the development of a behavioral intervention for women with FHA (6).…”

Section: Discussionmentioning

confidence: 99%

Synergism between psychosocial and metabolic stressors: impact on reproductive function in cynomolgus monkeys

Williams¹,

Berga²,

Cameron³

2007

American Journal of Physiology-Endocrinology and Metabolism

Self Cite

143

111

View full text Add to dashboard Cite

Williams NI, Berga SL, Cameron JL. Synergism between psychosocial and metabolic stressors: impact on reproductive function in cynomolgus monkeys. Am J Physiol Endocrinol Metab 293: E270-E276, 2007. First published April 3, 2007; doi:10.1152/ajpendo.00108.2007.-The role of energy imbalance versus psychosocial stress in the pathogenesis of female reproductive dysfunction characterized by anovulation and amenorrhea remains controversial. In women, functional hypothalamic amenorrhea can develop in the absence of significant weight loss, excessive exercise, or profound psychosocial disruption. We posited, therefore, that commonplace, seemingly minor stressors that alone would have minimal impact upon reproductive function might interact synergistically such that combinations of stressors would cause a greater impairment of the reproductive axis than any single stressor alone. We then developed a monkey model to test this hypothesis. Adult female cynomolgus monkeys with normal menstrual cycles were randomized into three experimental groups and studied over four menstrual cycles. The groups were: low-level psychosocial stress (i.e., moving to a new housing environment; Move, n ϭ 8), moderate energy imbalance (Exercise ϩ Diet, n ϭ 9); and all stressors in combination (Move ϩ Exercise ϩ Diet, n ϭ 10). Food intake, body weight, menstrual cyclicity, and reproductive hormones were assessed for two control menstrual cycles followed by two experimental cycles during which the monkeys experienced the stressors. Abnormal cycles were considered to be abnormally long or anovulatory cycles. Few abnormal cycles occurred in the Move group (1 of 8 monkeys) and in the Exercise ϩ Diet group (1 of 9 monkeys). In contrast, 7 of 10 monkeys in the Move ϩ Exercise ϩ Diet group displayed at least one abnormal cycle ( 2 ϭ 9.61, P ϭ 0.008). These findings suggest that infertility due to hypothalamic hypogonadism can result from the combination of commonplace, seemingly minor stressors that often escape clinical attention. exercise; diet; psychosocial stress; reproduction CLINICALLY RECOGNIZED FORMS of stress-induced reproductive dysfunction include functional hypothalamic amenorrhea (FHA), anorexia nervosa, bulimia nervosa, and exercise-associated amenorrhea. The proximate cause of these forms of reproductive compromise is a functional and theoretically reversible reduction in central drive to the reproductive axis provided by the hypothalamic neuroendocrine hormone gonadotropin-releasing hormone (GnRH). The presence of clinical forms of stress-induced reproductive dysfunction heightens the risk for other diseases, including cardiovascular disease, osteoporosis, depression, and other psychiatric conditions as well as infertility (1,15,20,28). Stress exposure during pregnancy can also have a negative impact on fetal development. Potential fetal consequences include preterm labor, poor neurodevelopment, and compromised psychosocial development (26, 31, 35).Although it was previously believed that different forms of stress-induced reproductive dy...

show abstract

Effects of Social Subordination on Macaque Neurobehavioral Outcomes: Focus on Neurodevelopment

Godfrey

Pincus

Sánchez

2016

Developments in Primatology: Progress and Prospects

View full text Add to dashboard Cite

Hypothalamic expression of serotonin 1A, 2A and 2C receptor and GAD67 mRNA in female cynomolgus monkeys with different sensitivity to stress

Cited by 27 publications

References 68 publications

Corticotropin-Releasing Hormone and Pro-Opiomelanocortin Gene Expression in Female Monkeys with Differences in Sensitivity to Stress

Corticotropin-Releasing Hormone and Pro-Opiomelanocortin Gene Expression in Female Monkeys with Differences in Sensitivity to Stress

Synergism between psychosocial and metabolic stressors: impact on reproductive function in cynomolgus monkeys

Effects of Social Subordination on Macaque Neurobehavioral Outcomes: Focus on Neurodevelopment

Contact Info

Product

Resources

About