Hypomethylated drm1 drm2 cmt3 mutant phenotype of Arabidopsis thaliana is related to auxin pathway impairment

Forgione, Ivano; Wołoszyńska, Magdalena; Pacenza, Marianna; Chiappetta, Adriana; Greco, Maria; Araniti, Fabrizio; Abenavoli, Maria Rosa; Lijsebettens, Mieke Van; Bitonti, Maria Beatrice; Bruno, Leonardo

doi:10.1016/j.plantsci.2018.12.029

Cited by 32 publications

(34 citation statements)

References 89 publications

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“…3e ). Consistent with an earlier report of msh1 epigenetic memory 16 and other studies 22 , 23 , these observations infer epigenomic responsiveness of phytohormone networks, particularly as relates directly or indirectly to auxin.…”

Section: Resultssupporting

confidence: 90%

MSH1-induced heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants

et al. 2020

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Plants transmit signals long distances, as evidenced in grafting experiments that create distinct rootstock-scion junctions. Noncoding small RNA is a signaling molecule that is graft transmissible, participating in RNA-directed DNA methylation; but the meiotic transmissibility of graft-mediated epigenetic changes remains unclear. Here, we exploit the MSH1 system in Arabidopsis and tomato to introduce rootstock epigenetic variation to grafting experiments. Introducing mutations dcl2, dcl3 and dcl4 to the msh1 rootstock disrupts siRNA production and reveals RdDM targets of methylation repatterning. Progeny from grafting experiments show enhanced growth vigor relative to controls. This heritable enhancement-through-grafting phenotype is RdDM-dependent, involving 1380 differentially methylated genes, many within auxin-related gene pathways. Growth vigor is associated with robust root growth of msh1 graft progeny, a phenotype associated with auxin transport based on inhibitor assays. Large-scale field experiments show msh1 grafting effects on tomato plant performance, heritable over five generations, demonstrating the agricultural potential of epigenetic variation.

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Section: Resultssupporting

confidence: 90%

MSH1-induced heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants

et al. 2020

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“…The accumulation and distribution of auxin in drm1-drm2-cmt3 mutants were affected [58]. DNA methylation is an epigenetic modification of DNA, which plays an important role in the development of embryo, organ, flower and fruit, and the response of plants to different stresses [59].…”

Section: Sto Had Crosstalk With Aba Upon Uv-b Radiationmentioning

confidence: 99%

“…DNA methylation is an epigenetic modification of DNA, which plays an important role in the development of embryo, organ, flower and fruit, and the response of plants to different stresses [59]. Mutations in DNA methyltransferase lead to DNA methylation loss, affect gene expression regulating auxin synthesis, transport and signaling pathways, and lead to developmental abnormalities [58]. DNA methyltransferase CMT3 and DRM2 were up-regulated to increase glutathione reductase (GR), ascorbic acid peroxidase (APX), and catalase (CAT) under Cu or Cd stress, which improve resistance to heavy metals in plants [60].…”

Section: Sto Had Crosstalk With Aba Upon Uv-b Radiationmentioning

confidence: 99%

Quantitative Proteomic Analyses Identify STO/BBX24 -Related Proteins Induced by UV-B

Lyu

Xiong

et al. 2020

IJMS

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Plants use solar radiation for photosynthesis and are inevitably exposed to UV-B. To adapt to UV-B radiation, plants have evolved a sophisticated strategy, but the mechanism is not well understood. We have previously reported that STO (salt tolerance)/BBX24 is a negative regulator of UV-B-induced photomorphogenesis. However, there is limited knowledge of the regulatory network of STO in UV-B signaling. Here, we report the identification of proteins differentially expressed in the wild type (WT) and sto mutant after UV-B radiation by iTRAQ (isobaric tags for relative and absolute quantitation)-based proteomic analysis to explore differential proteins that depend on STO and UV-B signaling. A total of 8212 proteins were successfully identified, 221 of them were STO-dependent proteins in UV-B irradiated plants. The abundances of STO-dependent PSB and LHC (light-harvesting complex) proteins in sto mutants decreased under UV-B radiation, suggesting that STO is necessary to maintain the normal accumulation of photosynthetic system complex under UV-B radiation to facilitate photosynthesis photon capture. The abundance of phenylalanine lyase-1 (PAL1), chalcone synthetase (CHS), and flavonoid synthetase (FLS) increased significantly after UV-B irradiation, suggesting that the accumulation of flavonoids do not require STO, but UV-B is needed. Under UV-B radiation, STO stabilizes the structure of antenna protein complex by maintaining the accumulation of PSBs and LHCs, thereby enhancing the non-photochemical quenching (NPQ) ability, releasing extra energy, protecting photosynthesis, and ultimately promoting the elongation of hypocotyl. The accumulation of flavonoid synthesis key proteins is independent of STO under UV-B radiation. Overall, our results provide a comprehensive regulatory network of STO in UV-B signaling.

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“…Due to functional redundancy, mutations in CMT3 and DRM genes do not lead to any visible phenotype. However, the triple mutant drm1 drm2 cmt3 was shown to exhibit a pleiotropic phenotype with alterations during embryonic and postembryonic development similar to met1 [46,48]. Most of the phenotypes found in this triple mutant, such as agravitropic root growth, leaf vascularity misconnection, and altered embryo development, were shown to be associated with alterations in auxin-related processes.…”

Section: Epigenetic Control Of Auxin Biosynthesis and Inactivationmentioning

confidence: 99%

Epigenetic Regulation of Auxin Homeostasis

2019

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Epigenetic regulation involves a myriad of mechanisms that regulate the expression of loci without altering the DNA sequence. These different mechanisms primarily result in modifications of the chromatin topology or DNA chemical structure that can be heritable or transient as a dynamic response to environmental cues. The phytohormone auxin plays an important role in almost every aspect of plant life via gradient formation. Auxin maxima/minima result from a complex balance of metabolism, transport, and signaling. Although epigenetic regulation of gene expression during development has been known for decades, the specific mechanisms behind the spatiotemporal dynamics of auxin levels in plants are only just being elucidated. In this review, we gather current knowledge on the epigenetic mechanisms regulating the expression of genes for indole-3-acetic acid (IAA) metabolism and transport in Arabidopsis and discuss future perspectives of this emerging field.

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Hypomethylated drm1 drm2 cmt3 mutant phenotype of Arabidopsis thaliana is related to auxin pathway impairment

Abstract: Hypomethylated drm1 drm2 cmt3 mutant phenotype of Arabidopsis thaliana is related to auxin pathway impairment

Cited by 32 publications

References 89 publications

MSH1-induced heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants

MSH1-induced heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants

Quantitative Proteomic Analyses Identify STO/BBX24 -Related Proteins Induced by UV-B

Epigenetic Regulation of Auxin Homeostasis

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