Hypoglycemic effect and toxicity of the dry extract of Eugenia biflora (L.) DC. leaves

Oliveira, Edinilze S.C.; Acho, Leonard Domingo Rosales; Silva, Bárbara Janaína P. da; Morales-Gamba, Ruben Dario; Pontes, Flávia Lada Degaut; Rosário, Alessandro Silva do; Bezerra, Jaqueline de Araújo; Campos, Francinete Ramos; Barcellos, José Fernando Marques; Lima, Émerson Silva; Machado, Marcos B.

doi:10.1016/j.jep.2022.115276

Cited by 5 publications

(11 citation statements)

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“…and were analysed using NMR (10.0 mg) in 530 μL of DMSO-d 6 , with tetramethylsilane (TMS) as an internal reference (0.0 ppm). 18 The 75 solutions were subjected to ultrasound for 5 min (25.0 C) and were then transferred to their respective NMR tubes (5 mm). A 90 pulse calibration was performed for all samples using the 90 pulse sequence (zg).…”

Section: Discussionmentioning

confidence: 99%

“…The preparation of the infusion followed the protocol described by our research group in two previously published studies involving the dry extracts of M. multiflora (MmAPA) and E. biflora (EbAPA). 16,18 The leaves of the species were dried in a circulating air oven (DeLeo Equipamentos Laboratoriais, Porto Alegre, RS, Brazil) for 120 h at the drying were carried out on the same day. The preparation of the infusions using the commercial samples followed the same previously described protocol (2.0%, m/v).…”

Section: Preparation Of the Dry Extractsmentioning

confidence: 99%

“…are able to inhibit the enzymes α-glucosidase and α-amylase, and also have free-radical-scavenging activities and inhibit advanced glycation end products (AGEs) in vitro. 5,[14][15][16][17][18] In addition, these extracts are able to lower the blood glucose levels in diabetic animals induced by streptozotocin and alloxan. 5,[17][18][19] The constituents present in these extracts, such as flavonoids, procyanidins, and ellagitannins, have been identified as being responsible for these pharmacological activities.…”

Section: Introductionmentioning

confidence: 99%

“…5,[14][15][16][17][18] In addition, these extracts are able to lower the blood glucose levels in diabetic animals induced by streptozotocin and alloxan. 5,[17][18][19] The constituents present in these extracts, such as flavonoids, procyanidins, and ellagitannins, have been identified as being responsible for these pharmacological activities.…”

Section: Introductionmentioning

confidence: 99%

“…9,21 Chemometric analyses have also been employed to explore data obtained via NMR, and have enabled the discrimination of samples of medicinal plants, 9,22 spices, 20 pediatric syrups, 21 green tea, 23 and herbal blends. 24 In two previously published studies, 16,18 we reported the identification, via NMR and HPLC-high-resolution mass spectrometry (HRMS), of the main chemical constituents of two species of pedraume-caá, M. multiflora and E. biflora. The methodology for the analysis proved to be reproducible and motivated us to investigate other matrices of pedra-ume-caá, as well as commercial samples, which were devoid of information regarding collection and botanical identification.…”

Section: Introductionmentioning

confidence: 99%

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NMR and multivariate methods: Identification of chemical markers in extracts of pedra‐ume‐caá and their antiglycation, antioxidant, and enzymatic inhibition activities

Oliveira,

Pontes,

Acho

et al. 2024

Phytochemical Analysis

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IntroductionIn Brazil, the plant group popularly known as “pedra‐ume‐caá” is used in folk medicine for the treatment of diabetes, and its raw material is commonly sold.ObjectiveThe aim of the study was to apply a method for chemical identification of extracts of dry pedra‐ume‐caá leaves using HPLC–high‐resolution mass spectrometry (HRMS) and NMR and develop a multivariate model with NMR data to authenticate commercial samples. In addition, to evaluate the biological activities of the extracts.Materials and MethodsDry extracts of Myrcia multiflora, Myrcia amazonica, Myrcia guianensis, Myrcia sylvatica, Eugenia punicifolia leaves, and 15 commercial samples (sold in Manaus and Belém, Brazil) were prepared by infusion. All the extracts were analysed using HPLC–high‐resolution mass spectrometry (HRMS), NMR, principal component analysis (PCA), and hierarchical cluster analysis (HCA). The antidiabetic effect of extracts was evaluated according to enzymatic inhibition. Their content of total phenols, cell viability, and antioxidant and antiglycation activities were also determined.ResultsHPLC‐HRMS and NMR analysis of these extracts permitted the identification of 17 compounds. 1H NMR data combined with multivariate analyses allowed us to conclude that catechin, myricitrin, quercitrin, and gallic and quinic acids are the main chemical markers of pedra‐ume‐caá species. These markers were identified in 15 commercial samples of pedra‐ume‐caá. Additionally, only the extracts of M. multiflora and E. punicifolia inhibited α‐glucosidase. All the extracts inhibited the formation of advanced glycation end products (AGEs) and showed free‐radical‐scavenging activity. These extracts did not present cytotoxicity.ConclusionThis study revealed the chemical markers of matrices, and it was possible to differentiate the materials marketed as pedra‐ume‐caá. Moreover, this study corroborates the potential of these species for treating diabetes.

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Section: Discussionmentioning

confidence: 99%

Section: Preparation Of the Dry Extractsmentioning

confidence: 99%