HYPOGLYCEMIC ACTIVITY OF FRUITING BODY EXTRACTS FROM Pycnoporus sanguineus (L.: Fr.) Murr. MUSHROOM

Abstract

ABSTRACT
 Alpha-amylase and α-glucosidase are two main enzymes involved in carbohydrate metabolism. Inhibition of α-amylase and α-glucosidase can be said as an effective treatment for delaying the absorption of glucose after meals in people with diabetes mellitus. The objective of the present study is to provide an in vitro evidence for the potential hypoglycemic activity via inhibitory activity of the ethanolic and aqueous extracts from fruiting bodies of Pycnoporus sanguineus mushroom on α-amylase and α-glucosidase enzymes. Alpha-amylase and α-glucosidase inhibitory activities of aqueous and ethanolic extracts of P. sanguineus fruiting body were examined in a dose-response manner. Acarbose was used as a positive control. The results showed that the ethanolic extract of P. sanguineus possessed strong inhibitory activity on α-amylase and α-glucosidase with IC50 values of 97.08 and 92.60 μg/mL, respectively. The aqueous extract also exhibited moderate activity against α-amylase and α-glucosidase with IC50 values of 150.15 and 102.29 μg/mL, respectively. Which was significantly lower than that of acarbose with IC50 values of 85.12 and 68.36 μg/mL, respectively. The degree of α-amylase and α-glucosidase inhibition was correlated with the dose of inhibitors. From these results Pycnoporus sanguineus (Trametes sanguinea) possesses high potential in lowering blood glucose level, reduced insulin resistance and the risk of diabetic-related complications.

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“…More generally, P. sanguineus has been used in empirical medicine in Africa [15] and South America to treat many diseases such as skin lesions, arthritis, gout, sore throat, ulcer, dental pain, fever and hemorrhage [16][17]. It has a high potential in reducing blood glucose levels and reduces insulin resistance and the risk of diabetes complications by inhibiting alpha-amylase and alpha-glucosidase [18]. According to Smânia et al [16], P. sanguineus has antifungal, antiradical, antimicrobial, antiviral and cytotoxic activities.…”

Expression of the Thermobifida fusca β-1,3-Glucanase in Yarrowia lipolytica and Its Application in Hydrolysis of β-1,3-Glucan from Four Kinds of Polyporaceae

“…β-glucans from various fungi vary greatly in length and the degree of branching. Although the flavonoids, saponins, tannins, and terpenes are antioxidant compounds in the mushroom [8]. Therefore, the antioxidant properties of fungi are primarily attributed to β-glucan [9].…”