Hypoglycemia as a Pathological Result in Medical Praxis

Bjelakovic, G.; Stojanović, Ivana; Jevtović-Stoimenov, Tatjana; Pavlović, Davor; Kocić, Gordana; Bjelakovic, B.

doi:10.5772/24754

Cited by 1 publication

(1 citation statement)

References 75 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was shown that the 3'-hydroxyl and 4'-hydroxyl group in the Ring B of the structure of a flavonoid, as well as the 3-hydroxyl residue in ring C, are very important for the inhibition of thrombin activity [51]. Ring B and C in flavonoids can interact with thrombin anchor S1 and S2, while ring A only partially interacts with S3 [64]. This suggests that the 3'-hydroxyl group and the 4'-hydroxyl group on the B ring of a flavonoid form hydrogen bond with amino acids to form the S1 anchor, which means that the B ring with hydroxyl groups at the position of R1 and R2 can mimic the arginine residue in P1 of thrombin substrates [65].…”

Section: Effect Of Hydroethanolic Extract On Clotting Timesmentioning

confidence: 99%

Laelia furfuracea Lindl.: an Endemic Mexican Orchid with Anticoagulant Activity

et al. 2021

View full text Add to dashboard Cite

Abstract. Laelia furfuracea is an endemic orchid from Mexico, with antioxidant activity. The objective of this study was to evaluate the effect of hydroethanolic extract and fractions obtained from the orchid leaves on the clotting times of patients with venous thromboembolic disease (VTD) and to identify their tentative compounds. The anticoagulant activity was evaluated by determining prothrombin (PT), thrombin (TT) and, activated partial thromboplastin (APTT) times. Identification of the compounds was carried out using a chromatographic technique with an ultra-high-performance liquid chromatographic analyzer coupled with electrospray ionization with quadrupole time of flight-mass-mass spectrometry. The extract prolonged the clotting times depending on the concentration-response (5-60 mg / mL); 25 mg/mL prolonged the PT (33.2 ± 2.3 s) and TT (33.1 ± 0.3 s); and APTT (61.8 ± 3.4 s) at a concentration of 15 mg/mL. The main groups tentatively identified were xanthine, carboxylic acid, amino acid, and phenolic compounds. These compounds or the synergy between them prolong clotting times. Laelia furfuracea is an orchid with research potential in the search for new anticoagulant agents. Resumen. Laelia furfuracea es una orquídea endémica de México, la cual posee actividad antioxidante. El objetivo de este estudio fue evaluar el efecto del extracto hidroetanólico y fracciones obtenidas de hojas de la orquídea sobre los tiempos de coagulación de pacientes con enfermedad tromboembólica venosa (ETV) e identificar sus posibles compuestos. La actividad anticoagulante se evaluó determinando los tiempos de protrombina (TP), trombina (TT) y tromboplastina parcial activada (TTPA). La identificación de los compuestos se realizó usando una técnica cromatográfica con un analizador cromatográfico líquido de Ultra Alta Resolución con Ionización por electroespray acoplado a espectrometría de masas con Cuadrupolo y Tiempo de Vuelo. El extracto prolongó los tiempos de coagulación dependiente de la concentración-respuesta (5-60 mg/mL); 25 mg/mL prolongó el TP (33.2±2.3 s) y TT (33.1±0.3 s); y TTPA (61.8±3.4 s) a una concentración de 15 mg/mL. Los principales grupos de posibles compuestos identificados fueron xantina, ácido carboxílico, aminoácido y compuestos fenólicos. Estos compuestos o la sinergia entre ellos prolongan los tiempos de coagulación. Laelia furfuracea es una orquídea con potencial en investigación para la búsqueda de nuevos agentes anticoagulantes.

show abstract

Section: Effect Of Hydroethanolic Extract On Clotting Timesmentioning

confidence: 99%