Hypoglycaemic effects of Mammea africana (Guttiferae) in diabetic rats

Tchamadeu, Marie Claire; Dzeufiet, Paul Désiré Djomeni; Nouga, C.C. Kouambou; Azebaze, Anatole Guy Blaise; Allard, Julien; Girolami, Jean-Pierre; Tack, Ivan; Kamtchouing, Pièrre; Dimo, Théophile

doi:10.1016/j.jep.2009.10.029

Cited by 35 publications

(23 citation statements)

References 11 publications

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“…The water and ethanol extracts were qualitatively phytochemically analyzed using the standard methods described by Cieule (1964); Harbone (1973); Mojab et al (2003) and Tchamadeu et al (2010) for presence of alkaloids salts (Meyer's and drangedorffs test), tannins (Styassny's reagent), saponins (foaming test), flavonoids (Shibata's reaction), reducing sugars (Fehling's tests), anthracenocides (Borntagen's reaction), coumarins (colour fluorescence under UV light), glycosides and triterpenoids (Liebermann-Burchard's test) and anthocynosides .…”

Section: Preliminary Phytochemical Screeningmentioning

confidence: 99%

In vitro anthelmintic potential of Vernonia amygdalina and Secamone africana on gastrointestinal nematodes

Nalule¹,

Mbaria²

2013

ABJNA

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In-vitro studies were conducted to determine the anthelmintic activity of ethanolic and water extracts of Vernonia amygdalina and Secamone africana used by agro-pastoralists in semi-arid land Uganda. The plant materials were collected from Nakasongola district and extracted using 70% ethanol and water. Efficacy and potency of crude extracts was determined using extracts' serial dilutions ranging 4mg/ml to 64mg/ml parallel to serial dilutions of albendazole ranging: 6.25-100mg/ml in three replicates. Ascaris suum model was used for the assays. Standard phytochemical methods were used for qualitative phytochemical analysis. The plants' extracts caused a dose-dependent motility inhibition with highest concentration of both ethanolic and water extracts of V.amygdalina causing 90% inhibition compared with 100% inhibition caused by albendazole. The corresponding median effective doses of ethanolic, water and albendazole were 5.94mg/ml, 13.70mg/ml and 15.12mg/ml respectively and significantly differed (F (2, 53) =257.43, p=0.001). There was a significant difference in motility inhibition in all dose levels (F (5, 53) = 14.01, P = 0.001; R 2 = 0.93). Similarly, the water and ethanolic extracts of S.africana caused a 93.3% and 80% motility inhibition with median effective doses of 40.08mg/ml and 25.41mg/ml respectively which also significantly differed (F (2, 53) =183.26, p= 0.001). There was also a highly significant difference in motility inhibition in all dose levels (F (5, 53) = 8.00, P = 0.001; R 2 = 0.92). The phytochemical screening revealed presence of tannins, alkaloid, saponins, flavonoids, steroids glycosides, triterpenes, coumarin derivatives, anthocyanocides, anthracenocides, and reducing sugars. In conclusion, V.amygdalina and S.africana are potential sources for novel antihelmintics and the secondary metabolites present justify their ethno-veterinary use.

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Section: Preliminary Phytochemical Screeningmentioning

confidence: 99%

In vitro anthelmintic potential of Vernonia amygdalina and Secamone africana on gastrointestinal nematodes

Nalule¹,

Mbaria²

2013

ABJNA

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“…To determine the chemical constituents, qualitative phytochemical screening of the aqueous stem bark extract of Pterocarpus soyauxii was carried out following standard procedures routinely used in the laboratory (Tchamadeu et al, 2010) and revealed alkaloids (Meyer and Dragendoff's test), tannins (FeCl 3 test), saponins (frothing test), lipids (Wattman paper test), flavonoids (Schinoda's test), glycosides and polyoses (NaCl, and Fehling's solutions A and B), anthraquinones (ether-chloroform and NaOH), phenols (FeCl 3 ), polyphenols (K 3 Fe(CN) 6 ), terpenoids (Liberman Burchard's test).…”

Section: Phytochemical Screeningmentioning

confidence: 99%

“…Whole blood cell (WBC), red blood cell (RBC), leukocyte and platelet counts, hematocrit, haemoglobin, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC), were determined as previously described (Tan et al, 2008), using an automatic analyser (System H1, Bayer Diagnostics). The serum was analysed as previously described (Tchamadeu et al, 2010) for total protein, cholesterol, creatinine, triglycerides and (ALT/AST) activities, using specific commercial diagnostic kits (Fortress Diagnostics, London, UK). Glutathione level was determined enzymatically (Ellman, 1959).…”

Section: Blood Analysismentioning

confidence: 99%

Acute and sub-chronic oral toxicity studies of an aqueous stem bark extract of Pterocarpus soyauxii Taub (Papilionaceae) in rodents

Tchamadeu

Dzeufiet

Nana

et al. 2011

Journal of Ethnopharmacology

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“…[11] The overall improvement on glucose tolerance with each of the plant extract over a period of 4h was evaluated using the total area under oral glucose tolerance curve, which has been followed by many authors. [1213]…”

Section: Discussionmentioning

confidence: 99%

Study of antihyperglycaemic activity of medicinal plant extracts in alloxan induced diabetic rats

et al. 2013

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Background:Diabetes mellitus, for a long time, has been treated with plant derived medicines in Sri Lanka.Aim:The aim of this study is to determine the efficacy and dose response of oral antihyperglycaemic activity of eight Sri Lankan medicinal plant extracts, which are used to treat diabetes in traditional medicine in diabetic rats.Materials and Methods:Medicinal plants selected for the study on the basis of documented effectiveness and wide use among traditional Ayurveda physicians in the Southern region of Sri Lanka for the treatment of diabetes mellitus. The effect of different doses of aqueous stem bark extracts of Spondias pinnata (Anacardiaceae), Kokoona zeylanica (Celastraceae), Syzygium caryophyllatum (Myrtaceae), Gmelina arborea (Verbenaceae), aerial part extracts of Scoparia dulcis (Scrophulariaceae), Sida alnifolia (Malvaceae), leaf extract of Coccinia grandis (Cucurbitaceae) and root extract of Languas galanga (Zingiberaceae) on oral glucose tolerance test was evaluated. A single dose of 0.25, 0.50, 0.75, 1.00, 1.25, 2.00 g/kg of plant extract was administered orally to alloxan induced (150 mg/kg, ip) diabetic Wistar rats (n = 6). Glibenclamide (0.50 mg/kg) was used as the standard drug. The acute effect was evaluated over a 4 h period using area under the oral glucose tolerance curve.Statistical Analysis:The results were evaluated by analysis of variance followed by Dunnett's test.Results:The eight plant extracts showed statistically significant dose dependent improvement on glucose tolerance (P < 0.05). The optimum effective dose on glucose tolerance for six extracts was found to be 1.00 g/kg in diabetic rats with the exception of C. grandis: 0.75 g/kg and L. galanga: 1.25 g/kg.Conclusion:The aqueous extract of G. arborea, S. pinnata, K. zeylanica, S. caryophyllatum, S. dulcis, S. alnifolia, L. galanga and C. grandis possess potent acute antihyperglycaemic activity in alloxan induced diabetic rats.

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Hypoglycaemic effects of Mammea africana (Guttiferae) in diabetic rats

Cited by 35 publications

References 11 publications

In vitro anthelmintic potential of Vernonia amygdalina and Secamone africana on gastrointestinal nematodes

In vitro anthelmintic potential of Vernonia amygdalina and Secamone africana on gastrointestinal nematodes

Acute and sub-chronic oral toxicity studies of an aqueous stem bark extract of Pterocarpus soyauxii Taub (Papilionaceae) in rodents

Study of antihyperglycaemic activity of medicinal plant extracts in alloxan induced diabetic rats

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