Hypogalactosylation of Salivary and Gingival Fluid Immunoglobulin G in Patients With Advanced Periodontitis

Stefanović, G; Marković, Dragana; Ilić, Vesna; Brajović, Gavrilo; Petrović, Sonja; Milosević-Jovcić, N

doi:10.1902/jop.2006.060049

Cited by 20 publications

(21 citation statements)

References 17 publications

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“…The microenvironment of the periodontal pocket contains an abundance of sialylated glycoproteins such as fibronectin and immunoglobulins, found in saliva and gingival crevicular fluid (56). While a number of periodontal bacteria are known to exploit host sialylated glycoproteins as a nutrient source, sialidase activity can also play an important role in their pathogenicity (3,4,10,17,28).…”

Section: Discussionmentioning

confidence: 99%

Filifactor alocis Has Virulence Attributes That Can Enhance Its Persistence under Oxidative Stress Conditions and Mediate Invasion of Epithelial Cells by Porphyromonas gingivalis

2011

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Filifactor alocis, a Gram-positive anaerobic rod, is one of the most abundant bacteria identified in the periodontal pockets of periodontitis patients. There is a gap in our understanding of its pathogenicity and ability to interact with other periodontal pathogens. To evaluate the virulence potential of F. alocis and its ability to interact with Porphyromonas gingivalis W83, several clinical isolates of F. alocis were characterized. F. alocis showed nongingipain protease and sialidase activities. In silico analysis revealed the molecular relatedness of several virulence factors from F. alocis and P. gingivalis. In contrast to P. gingivalis, F. alocis was relatively resistant to oxidative stress and its growth was stimulated under those conditions. Biofilm formation was significantly increased in coculture. There was an increase in adherence and invasion of epithelial cells in coculture compared with P. gingivalis or F. alocis monocultures. In those epithelial cells, endocytic vesiclemediated internalization was observed only during coculture. The F. alocis clinical isolate had an increased invasive capacity in coculture with P. gingivalis compared to the ATCC 35896 strain. In addition, there was variation in the proteomes of the clinical isolates compared to the ATCC 35896 strain. Hypothetical proteins and those known to be important virulence factors in other bacteria were identified. These results indicate that F. alocis has virulence properties that may enhance its ability to survive and persist in the periodontal pocket and may play an important role in infection-induced periodontal disease.

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Section: Discussionmentioning

confidence: 99%

Filifactor alocis Has Virulence Attributes That Can Enhance Its Persistence under Oxidative Stress Conditions and Mediate Invasion of Epithelial Cells by Porphyromonas gingivalis

2011

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“…As the presence of N-linked sugar chains was found to play a crucial role in IgG effector functions [1,2,8], there has been an increasing interest in the analysis of the N-glycosylation profile(s) of human IgG in health and a number of disease states, such as infections, inflammation and autoimmunity [10][11][12][13][14][15][16][17][18]. However, less is known about the potential role of the Fc glycans in malignancy and tumor immunity.…”

Section: Introductionmentioning

confidence: 99%

Immunoglobulin G Fc N-glycan profiling in patients with gastric cancer by LC-ESI-MS: relation to tumor progression and survival

et al. 2011

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The IgG Fc glycans strongly influence the Fcγ receptor interactions and Fc-mediated effector mechanisms. Changes in the structure of IgG glycans are associated with various diseases, such as infections and autoimmunity. However, the possible role of Fc glycans in tumor immunity is not yet fully understood. The aim of this study was to profile the Fc N-glycans of IgG samples from patients with gastric cancer (n = 80) and controls (n = 51) using LC-ESI-MS method to correlate the findings with stage of cancer and patients survival. Analysis of 32 different IgG N-glycans revealed significant increase of agalactosylated (GnGnF, GnGn(bi)F), and decrease of galactosylated (AGn(bi), AGn(bi)F, AA(bi), AAF) and monosialylated IgG glycoforms (NaAF, NaA(bi)) in cancer patients. A statistically significant increase of Fc fucosylation was observed in tumor stage II and III whereas reverse changes were found for the presence of bisecting GlcNAc. Higher level of fully sialylated glycans and elevated expression of glycans with bisecting GlcNAc were associated with better survival rate. Our findings provide the first evidence that the changes in Fc glycan profile may predict the survival of patients with gastric cancer. Cancer stage-dependent changes in Fc fucosylation and the bisecting N-acteylglucosamine expression as well as an association of several IgG glycoforms with the survival suggest that IgG glycosylation is related to pathogenesis of cancer and progression of the disease.

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“…La bolsa periodontal representa una mayor cantidad y variedad de bacterias periodontopatógenas que en la gingivitis. Estudios como los de Reinharrdt y cols (1989) 14 , Lamster y cols (1990) 15 , Ebersole (1994) 16 y Stefanovic y cols (2000) 13 han medido la concentración de IgG en fluido crevicular demostrando su elevación en condiciones periodontalmente patógenas. Es así que a mayor profundidad de surco gingival existe mayor cantidad de fluido crevicular que puede se vaciado en la saliva junto con los diversos marcadores presentes en éste.…”

Section: Discussionunclassified

Niveles de inmunoglobulina G en saliva como marcador biológico de la enfermedad periodontal

Bravo-castagnola

García-Linares

Bonilla-Ferreyra

2014

Odontol. sanmarquina

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Niveles de inmunoglobulina G en saliva como marcador biológico de la enfermedad periodontal Inmunoglobulin G levels in saliva as biological marker in periodontal disease Resumen El presente estudio evaluó la concentración de inmunoglobulina G (IgG) en saliva total y su papel como marcador biológico en la enfermedad periodontal. Se incluyeron 50 individuos sanos y 40 pacientes con enfermedad periodontal como grupos control y de estudio respectivamente. El grupo de estudio se dividió en subgrupos de 20 individuos, gingivitis y periodontitis. El examen clínico evaluó placa dentaria, sangrado al sondaje y profundidad de sondaje antes y después de la Fase I del tratamiento. En ambos momentos se tomaron muestras de saliva total para determinar la concentración de IgG mediante el análisis espectrofotométrico de su absorbancia. Se encontró que a mayor progresión de la enfermedad periodontal los niveles de IgG eran más elevados encontrándose diferencias significativas entre pacientes sanos, con gingivitis y periodontitis. Luego del tratamiento los valores de pacientes con gingivitis disminuyeron significativamente alcanzando valores normales. En los individuos con periodontitis los valores disminuyeron significativamente pero sin alcanzar valores normales. Asimismo, la variación de los niveles de IgG se correlacionaba más fuertemente con la profundidad de sondaje en relación a los otros parámetros diagnósticos. Se concluyó que los niveles de IgG en saliva total son marcadores biológicos importantes en la evaluación de la presencia, progresión y efectividad del tratamiento en la enfermedad periodontal Abstract This study evaluated the concentration of immunoglobulin (IgG) in saliva and its role as a biological marker in periodontal disease. For this study, 50 healthy people and 40 patients with periodontal disease were included as control and study group respectively. The study group was divided in gingivitis and periodontitis subgroups with 20 patients each. The clinical examination evaluated, dental plaque, probing bleeding and probing depth before and after the first phase of treatment. It was taken samples of saliva to determine the IgG concentration through spectrophotometric analysis of its absorbance, in both moments. We found, that the greater the progression of periodontal disease, the most elevated IgG level, showing statistical significant differences between healthy group and gingivitis and periodontitis groups. After treatment, gingivitis group scores decreased until they reached normal rates. The periodontitis group scores, decreased, but not to levels of healthy group. IgG level variations was strongly related to probing depth diagnostic parameter than the other two. We concluded that, IgG levels in total saliva are important biological markers for evaluating periodontal disease's presence, progression, treatment and effectiveness. IntroduccIÓnEn los últimos años el entendimiento, diagnóstico y tratamiento de la enfermedad periodontal han avanzado notablemente. Es así que muchas investigaciones relacionadas...

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Hypogalactosylation of Salivary and Gingival Fluid Immunoglobulin G in Patients With Advanced Periodontitis

Abstract: The results suggest that the shift toward hypogalactosylated glycoforms may occur during the process of inflammation of the gingiva.

Cited by 20 publications

References 17 publications

Filifactor alocis Has Virulence Attributes That Can Enhance Its Persistence under Oxidative Stress Conditions and Mediate Invasion of Epithelial Cells by Porphyromonas gingivalis

Filifactor alocis Has Virulence Attributes That Can Enhance Its Persistence under Oxidative Stress Conditions and Mediate Invasion of Epithelial Cells by Porphyromonas gingivalis

Immunoglobulin G Fc N-glycan profiling in patients with gastric cancer by LC-ESI-MS: relation to tumor progression and survival

Niveles de inmunoglobulina G en saliva como marcador biológico de la enfermedad periodontal

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