Hypodermin A, a trypsin-like neutral proteinase from the insect Hypoderma lineatum

Tong, Nguyen Thanh; Imhoff, Jean-Marie; Lecroisey, Anne; Keil, B.

doi:10.1016/0005-2744(81)90291-6

Cited by 39 publications

(7 citation statements)

References 27 publications

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“…First instars of the Hypodermatinae penetrate the skin and migrate through the host's conjunctive tissue aided by enzymatic secretions. Different studies on larval secretions of both species of Hypoderma affecting cattle, H. lineatum and H. bovis , have described and characterized three serine proteases called hypodermin A, B and C (Boulard & Garrone, 1978; Lecroisey et al , 1979, 1983; Tong et al , 1981).…”

Section: Introductionmentioning

confidence: 94%

Use of a crude extract or purified antigen from first-instar cattle grubs,Hypoderma lineatum, for the detection of anti-Hypodermaantibodies in free-ranging cervids from southern Spain

Fontán

Martínez‐Carrasco

León-Vizcaíno

et al. 2010

Medical and Veterinary Entomology

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During the 2003-2005 hunting seasons, a total of 120 Cervidae, including 39 red deer (Cervus elaphus hispanicus) and 81 fallow deer (Dama dama), were examined for subcutaneous myiasis. Animals were shot from January to June in southern Spain. Specific antibodies against Hypodermatinae (Diptera: Oestridae) were detected by indirect enzyme-linked immunosorbent assay (iELISA) using a crude larval extract (CLE) and a purified antigen [hypodermin C (HC)] obtained from first instars of Hypoderma lineatum (De Villers) (Diptera: Oestridae). Hypoderma actaeon Brauer was the only species detected in this study, which represents the first confirmation of this species in fallow deer from Spain. The overall prevalence of animals presenting subcutaneous larvae (14.2%) was considerably lower than the prevalences determined by iELISA with CLE (43.3%) and HC (40.0%). Red deer showed a higher prevalence of Hypoderma than fallow deer. The concordance between larval examination during the hunting season and iELISA using both antigens was low, whereas the concordance between the CLE and HC ELISAs was good. Larval antigens obtained from H. lineatum constitute a good tool for the diagnosis of H. actaeon in Cervidae, especially when the hunting season does not coincide with the maximum presence of larvae on the back.

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Section: Introductionmentioning

confidence: 94%

Use of a crude extract or purified antigen from first-instar cattle grubs,Hypoderma lineatum, for the detection of anti-Hypodermaantibodies in free-ranging cervids from southern Spain

Fontán

Martínez‐Carrasco

León-Vizcaíno

et al. 2010

Medical and Veterinary Entomology

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“…This protein has been extensively studied and its nucleotide sequence has been determined (Moiré et al 1994). It is a serine protease related to the trypsin family (Tong et al 1981) . We and others have shown that HA inhibits the proliferation of lymphocytes activated by different mitogens. Recent studies (Nicolas-Gaulard, Moiré & Boulard 1995) described that the inhibition of PHA-induced proliferation was provoked by an increase in prostaglandin E2 (PGE 2 ) production and the subsequent inhibition of interleukin-2 (IL-2) secretion.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic effect of hypodermin A, a parasite protease, on bovine lymphocyte membrane antigens

Moiré

Nicolas-Gaulard

Vern

et al. 1997

Parasite Immunology

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The protease hypodermin A (HA) is produced by the parasitic warble-fly larva and is implicated in the modulation of the bovine immune system. This study examines the effect of this enzyme on the cell surface markers of bovine lymphocytes. HA interfered with the binding of all anti-lymphocyte receptor antibodies tested. Anti-BoCD2 and CD5 staining was completely abolished. But the mean fluorescence intensity (MFI) only was diminished for antibodies against BoCD4, CD8 and CD18. On the contrary, the MFI for anti-MHC Cl I molecules staining was increased. This effect of HA began as early as one h, and was reversed by removal of HA. Heating or PMSF treatment, which both inhibit protease activity, abolished the action of HA on the surface antigens. The HA concentrations (100 micrograms/ml) needed to alter antibody binding were similar to those that inhibited phytohaemagglutinin (PHA)-induced proliferation. These results show that enzymatic activity of HA on lymphocyte surface markers may be implicated in the inhibition of lymphocyte proliferation.

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“…This route of infection has also been suggested for myiases caused by the genera Gasterophilus and Cuterebra (Medownick et al, 1985;Anderson, 2006). Larval migration takes place as a consequence of the mechanical action of the cephaloskeleton, as well as of the proteolytic enzymes secreted by the larvae (i.e., serine proteases) (Boulard and Garrone, 1978;Lecroisey et al, 1979Lecroisey et al, , 1983Tong et al, 1981). Once the larvae reach the ocular region, they enter the sclera (Kearney et al, 1991) and the subretinal space, possibly causing hypopigmented subretinal tracks.…”

Section: Routes Of Infectionmentioning

confidence: 81%

Arthropods affecting the human eye

Fontán

Otranto

2015

Veterinary Parasitology

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Hypodermin A, a trypsin-like neutral proteinase from the insect Hypoderma lineatum

Cited by 39 publications

References 27 publications

Use of a crude extract or purified antigen from first-instar cattle grubs,Hypoderma lineatum, for the detection of anti-Hypodermaantibodies in free-ranging cervids from southern Spain

Use of a crude extract or purified antigen from first-instar cattle grubs,Hypoderma lineatum, for the detection of anti-Hypodermaantibodies in free-ranging cervids from southern Spain

Enzymatic effect of hypodermin A, a parasite protease, on bovine lymphocyte membrane antigens

Arthropods affecting the human eye

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