Hypocrea virens sp. nov., the Teleomorph of Trichoderma virens

Chaverri, Priscila; Samuels, Gary J.; Stewart, Elwin L.

doi:10.2307/3761672

Cited by 36 publications

(23 citation statements)

References 24 publications

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“…virens was first reported from Indiana, USA from a decorticated wood, and it has been found to be the telemorph of T. virens according to molecular and morphological characters (Chaverri et al, 2001). H. virens has generally been found earlier from soil, decaying wood, on other fungi (such as basidiomycetes) and as opportunistic plant symbionts (Herman et al, 2004;Druzhinina et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial constituents of Hypocrea virens, an endophyte of the mangrove-associate plant Premna serratifolia L.

Ratnaweera¹,

Silva²,

Wijesundera³

et al. 2016

J. Natn. Sci. Foundation Sri Lanka

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Emergence of multidrug-resistant pathogenic microorganisms has prompted a worldwide search for new antibiotics from various sources. Endophytic fungi from unique habitats are considered as potential sources of novel bioactive compounds. Sri Lankan mangrove ecosystem is such a distinctive and unexploited resource for the discovery of structurally diverse and biologically active metabolites including antimicrobials. Nine endophytic fungi were isolated from the leaves and twigs of Premna serratifolia L. from a mangrove habitat in the Negombo lagoon and the antimicrobial activities of their laboratory cultures were evaluated. The most promising antimicrobial activity was exhibited by the endophytic fungus Hypocrea virens. Bioassay guided fractionation of the organic extract of this fungus led to the isolation of two known metabolites; the antimicrobial epidithiodioxopiperazine, gliotoxin (1), and the closely related but less active bisdethiobis(methylthio)gliotxin (2). The chemical structures of the two compounds were determined by spectroscopy and confirmed by comparison of mass and nuclear magnetic resonance (NMR) spectral data with the reported values for these molecules. The minimum inhibitory concentration (MIC) values obtained for gliotoxin (1) in the current study are 0.13 µg mL -1 for Bacillus subtilis, 16 µg mL -1 for Staphylococcus aureus, 32 µg mL -1 for Methicillin Resistant Staphylococcus aureus (MRSA) and Escherichia coli, 64 µg mL -1 for Pseudomonas aeruginosa and Candida albicans fungus. This is the first study to report the isolation of endophytic fungi from P. serratifolia and their antimicrobial activities.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial constituents of Hypocrea virens, an endophyte of the mangrove-associate plant Premna serratifolia L.

Ratnaweera¹,

Silva²,

Wijesundera³

et al. 2016

J. Natn. Sci. Foundation Sri Lanka

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“…Ex Fr. (Chaverri et al, 2001). Estudos culturais comprovam que muitas espécies de Hypocrea produzem a forma conidial (Trichoderma ), mas o oposto não é verdadeiro para a maioria das espécies (Gams et al, 1980).…”

Section: Discussionunclassified

“…(Chaverri et al, 2000). O teleomorfo de T. virens (Miller, Giddens & Foster) v.Arx foi recentemente descrito (Chaverri et al, 2001) como Hypocrea virens Chaverri, Samuels & Stewart.…”

Section: Introductionunclassified

Hypocrea stromatica sp. nov. teleomorfo de Trichoderma stromaticum

et al. 2003

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Trichoderma stromaticum é um fungo antagonista a Crinipellis perniciosa, agente causal da vassoura-de-bruxa do cacaueiro (Theobroma cacao). Estromas periteciais de Hypocrea sp. formaram-se sobre a superfície de frutos mumificados e vassouras secas de cacaueiros em áreas onde T. stromaticum tinha sido pulverizado, experimentalmente, no controle biológico de C. perniciosa. Ascósporos obtidos dos estromas de Hypocrea sp. originaram colônias idênticas às de T. stromaticum. DNA genômico de colônias provenientes de ascósporos desta Hypocrea sp., de conídios de T. stromaticum e de Fusarium sp. (controle negativo) foi extraído e amplificado com oito "primers" decâmeros para obtenção de marcadores RAPD. Observaram-se similaridades genéticas de 0,82 a 0,96 entre os isolados ascospóricos e conidiais com base nos marcadores RAPD e de 0,06 a 0,09 entre estes e o isolado de Fusarium sp. O teleomorfo de T. stromaticum é denominado H. stromatica Bezerra, Costa & Bastos sp. nov.

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“…The internal transcribed spacer (ITS-1) and internal transcribed spacer (ITS-2) region of 5.8Sr DNA and tef-1 (gene) of the five Trichoderma virens isolates were analyzed (Chaverri et al, 2001). Hermosa et al, 2004, attempted to analyze the genetic variability within bio-control isolates of Trichoderma using sequence data obtained from the ITS Trichoderma species is one of the key potential bio-control agents against soil-borne pathogens.…”

Section: Introductionmentioning

confidence: 99%