Hypocholesterolemic effect of β-caryophyllene in rats fed cholesterol and fat enriched diet

Harb, Amani A.; Bustanji, Yasser; Abdalla, Shtaywy

doi:10.3164/jcbn.17-3

Cited by 39 publications

(39 citation statements)

References 41 publications

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“…In contrast to the studies described herein, other investigators have reported hypolipidemic effects of BCP [58][59][60][61]. These observations were detected, in vivo, under both hyperlipidemic and hypercholesterolemic conditions.…”

Section: Resultscontrasting

confidence: 98%

β-caryophyllene enhances the transcriptional upregulation of cholesterol biosynthesis in breast cancer cells

Mboge¹,

Bullock²,

O’Donnell³

et al. 2019

Current Topics in Biochemical Research

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Supplementary Figure LegendsSupplementary Figure Legends Supplementary Figure 1. Paclitaxel reduces growth of aggressive breast cells. Panels A and B. UFH-001, MDA-MB-231 LM2, T47D, or MCF10 A cells were exposed to specific concentrations of Paclitaxel for 24 h (Panel A) or 48 (Panel B). The MTT assay was then used to assess viability/cell growth. Data are reported as percent of control: avg ± S.E.M., n = 3. Panel C. LDH activity was measured in the medium of cells treated with specific concentrations of Paclitaxel for 32 h followed by exposure to 70 µM BCP or vehicle for 16 h. Data are reported as the avg ± S.E.M., n=3. Panel D. Under the same conditions as in Panel C, the MTT assay was performed at 48 to assess viability/cell growth. Data are presented at OD at 570: avg ± S.E.M., n = 3. Supplementary Figure 2. Cytotoxicity of BCP is not affected by hypoxia. Cells were exposed to normoxic or hypoxic condition in the presence of increasing concentrations of BCP. LDH release was measured after 16 h. Data shown represent the average of at least 3 biological replicates ± S.E.M.

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Section: Resultscontrasting

confidence: 98%

β-caryophyllene enhances the transcriptional upregulation of cholesterol biosynthesis in breast cancer cells

Mboge¹,

Bullock²,

O’Donnell³

et al. 2019

Current Topics in Biochemical Research

View full text Add to dashboard Cite

show abstract

“…In contrast to the studies described herein, other investigators have reported hypolipidemic effects of BCP (58)(59)(60)(61). These observations were detected, in vivo, under both hyperlipidemic and hypercholesterolemic conditions.…”

Section: Is Cholesterol Content or Redistribution Within The Pm The Kcontrasting

confidence: 99%

β-Caryophyllene Enhances the Transcriptional Upregulation of Cholesterol Biosynthesis in Breast Cancer Cells

Mboge

Ramirez-Mata

Bullock

et al. 2019

Preprint

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β-caryophyllene (BCP) exhibits anti-proliferative properties in cancer cells. Here, we examine the hypothesis that BCP induces membrane remodeling. Our data show that high concentrations of BCP increase membrane permeability of human breast cells (hBrC) causing detachment and cell death. At a sub-lethal concentration of BCP, we show that BCP induces a striking upregulation of genes involved in cholesterol biosynthesis, including the gene that encodes for HMGCoA reductase (HMGCR), the ratedetermining step in cholesterol biosynthesis. In addition, stearoyl-CoA desaturase (SCD) is also upregulated which would lead to the enhanced formation of monounsaturated fatty acids, specifically oleate and palmitoleate from stearoyl CoA and palmitoyl CoA, respectively. These fatty acids are major components of membrane phospholipids and cholesterol esters. Together, these data suggest that cells respond to BCP by increasing the synthesis of components found in membranes. These responses could be viewed as a repair mechanism and/or as a mechanism to mount resistance to the cytotoxic effect of BCP. Blocking HMGCR activity enhances the cytotoxicity of BCP, suggesting that BCP may provide an additional therapeutic tool in controlling breast cancer cell growth.

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“…Moreover, the caffeoylquinic acid had been reported to have antihyperlipidemic mechanism by increasing the expression of PPARalpha and PPARdelta, suppressing adiponectin, and upregulation of LPL and AMPK activities [46][47][48]. Furthermore, it had been reported that beta-caryophyllene, one of the main components in the PIT, could attenuate palmitate-induced lipid accumulation through AMPK signaling and prevent atherosclerosis in hypercholesterolemic rats [49][50][51][52].…”

Section: Discussionmentioning

confidence: 99%

Pluchea indica (L.) Less. Tea Ameliorates Hyperglycemia, Dyslipidemia, and Obesity in High Fat Diet‐Fed Mice

Sirichaiwetchakoon

Lowe

Kupittayanant

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Pluchea indica (L.) Less. (P. indica) tea has been used for a health-promoting drink, especially in Southeast Asia. The effect of P. indica tea (PIT) on amelioration of hyperglycemia; dyslipidemia that was total cholesterol (TC), LDL-cholesterol (LDL-C), HDL-cholesterol (HDL-C), and triglyceride (TG); and obesity in high fat diet-induced (HFD) mice was investigated. Oral glucose tolerance test (OGTT) displayed that PIT at 400 and 600 mg/kg orally ameliorated hyperglycemia with a dose-dependent manner compared to the untreated group. Moreover, PIT at these dosages exhibited significantly lower TC, LDL-C, TG, and perigonadal fat weight in HFD treated mice compared to HFD mice (P<0.05) with a dose-dependent manner. In contrast, HDL-C was higher than in the HFD group, but not a significant difference (P>0.05). The PIT chemical analysis results demonstrated that PIT contained total phenolic content (TPC), total flavonoid content (TFC), 4-O-caffeoylquinic acid (4-CQ), 5-O-caffeoylquinic acid (5-CQ), 3,4-O-dicaffeoylquinic acid (3,4-CQ), 3,5-O-dicaffeoylquinic acid (3,5-CQ), 4,5-O-dicaffeoylquinic acid (4,5-CQ), beta-caryophyllene, and gamma-gurjunene that may play an important role in inhibiting hyperlipidemia and hyperglycemia. Also, histological analysis expressed that the mean area and amount of perigonadal fat adipocytes of PIT treated groups were significantly lower and higher than the HFD group (P<0.05), respectively. The toxicity test of PIT at 600 mg/kg/day in mice showed that serum creatinine, alanine transaminase (ALT), alkaline phosphatase (ALP), and complete blood count (CBC) levels of HFD and PIT treated groups were not significantly different compared to the normal control diet group (NCD) (P>0.05). These results suggest that PIT does not become toxic to the kidney, liver, and blood. In conclusion, PIT has the potential to develop into healthy food supplement or medicine for the prevention and treatment of hyperglycemic, hyperlipidemic, and obese patients.

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Hypocholesterolemic effect of β-caryophyllene in rats fed cholesterol and fat enriched diet

Cited by 39 publications

References 41 publications

β-caryophyllene enhances the transcriptional upregulation of cholesterol biosynthesis in breast cancer cells

β-caryophyllene enhances the transcriptional upregulation of cholesterol biosynthesis in breast cancer cells

β-Caryophyllene Enhances the Transcriptional Upregulation of Cholesterol Biosynthesis in Breast Cancer Cells

Pluchea indica (L.) Less. Tea Ameliorates Hyperglycemia, Dyslipidemia, and Obesity in High Fat Diet‐Fed Mice

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