Hypochlorous acid induced structural and conformational modifications in human DNA: A multi-spectroscopic study

Tantry, Irfan Qadir; Waris, Sana; Habib, Safia; Khan, Rizwan Hasan; Mahmood, Riaz; Ali, Asif

doi:10.1016/j.ijbiomac.2017.08.051

Cited by 23 publications

(4 citation statements)

References 34 publications

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“…The oxidation of viral proteins was discernible above the 1.6 ppm threshold, and a reduction in infectious titer and protein aggregation was observable at approximately 0.6 ppm, implying that protein aggregation alone is adequate for viral inactivation. Nucleic acids are an intriguing potential target for HClO treatment ( Hawkins and Davies, 2002 ; Jiang et al, 2003 ), whereby nucleic chloramine formation produces mutagenic, genotoxic, and cytotoxic effects ( Bernofsky, 1991 ; Tantry et al, 2018 ). Notably, no oxidation was observed in the viral RNA of the purified viral particles treated with Hp-SA-HAW ( Figure 3A ), and the purified viral RNA from the particles treated with Hp-SA-HAW displayed compatible infectivity following transfection into cells ( Figure 3B ).…”

Section: Discussionmentioning

confidence: 99%

Electrolyzed hypochlorous acid water exhibits potent disinfectant activity against various viruses through irreversible protein aggregation

Dianty,

Hirano,

Anzai

et al. 2023

Front. Microbiol.

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It is essential to employ efficient measures to prevent the transmission of pathogenic agents during a pandemic. One such method involves using hypochlorous acid (HClO) solution. The oxidative properties of HClO water (HAW) can contribute to its ability to eliminate viral particles. Here, we examined a highly purified slightly acidic hypochlorous acid water (Hp-SA-HAW) obtained from the reverse osmosis membrane treatment of an electrolytically-generated SA-HAW for its anti-viral activity and mode of action on viral proteins. Hp-SA-HAW exhibited broad-spectrum antiviral effects against various viruses, including adenovirus, hepatitis B virus, Japanese encephalitis virus (JEV), and rotavirus. Additionally, Hp-SA-HAW treatment dose-dependently resulted in irreversibly aggregated multimers of the JEV envelope and capsid proteins. However, Hp-SA-HAW treatment had no discernible effect on viral RNA, indicating that Hp-SA-HAW acts against amino acids rather than nucleic acids. Furthermore, Hp-SA-HAW substantially reduced the infectivity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including the ancestral variant and other multiple variants. Hp-SA-HAW treatment induced the aggregation of the SARS-CoV-2 spike and nuclear proteins and disrupted the binding of the purified spike protein of SARS-CoV-2 to human ACE2. This study demonstrates that the broad-spectrum virucidal activity of highly purified HClO is attributed to viral protein aggregation of virion via protein oxidation.

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Section: Discussionmentioning

confidence: 99%

Electrolyzed hypochlorous acid water exhibits potent disinfectant activity against various viruses through irreversible protein aggregation

Dianty,

Hirano,

Anzai

et al. 2023

Front. Microbiol.

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“…Содержание хлора в исходных растворах составило 801 и 1566 mg/L. Концентрацию гипохлорита натрия в водном растворе определяли спектрофотометрически, используя коэффициент экстинкции ε 292 = 350 М −1 см −1 [13]. Концентрация гипохлорита натрия во всех комплексах составляла 7.3 • 10 −3 M.…”

Section: материалы и методыunclassified

Влияние структурной организации нуклеиновых кислот на взаимодействие с гипохлоритом: АТФP, PolyA и ДНК

Moroshkina¹,

Осинникова²,

Pavlova³

et al. 2023

Оптика И Спектроскопия

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The action of hypochlorite on various biological molecules in a living cell has been actively studied for years. However, the influence of the structural organization of nucleic acids on their interaction with hypochlorite remains underinvestigated. In this work, using ultraviolet and infrared spectroscopy, we analyzed the effect of the structure of nucleic acids on the reaction with hypochlorite using the example of the three most common and biologically significant types of nucleic acids (NA): double-stranded DNA in the B-form, single-stranded RNA, and nucleotide phosphates. It was found that the rate of the initial stage of the reaction of hypochlorite with endocyclic nitrogen atoms depends on the presence/absence of base pairing in the NA structure. At the same time, the polymeric structure of NC significantly accelerates and increases the efficiency of the subsequent stages of the reaction associated with the chlorination of exocyclic nitrogen atoms and the destruction of the ring structure of nitrogenous bases.

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“…This is particularly important in cancers related to cigarette smoke and patients with lung cancer, which have elevated MPO levels in lung tissue and serum. MPO-generated HOCl can cause structural changes, strand breaks and impact DNA binding properties, thereby leading to DNA irregularities, which ultimately may promote cancer progression [ 96 ]. As mentioned above, other MPO products such as HOBr and HOCl can oxidize components of DNA, resulting in the generation of 5-bromouracil, 5-chlorocytosine and other DNA-centered radicals and this has been proposed for consideration as a potential therapeutic target to prevent MPO-associated carcinogen formation [ 152 , 153 ].…”

Section: Mpo and Cancermentioning

confidence: 99%

Non-Canonical Functions of Myeloperoxidase in Immune Regulation, Tissue Inflammation and Cancer

Lockhart

Sumagin

2022

IJMS

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Myeloperoxidase (MPO) is one of the most abundantly expressed proteins in neutrophils. It serves as a critical component of the antimicrobial defense system, facilitating microbial killing via generation of reactive oxygen species (ROS). Interestingly, emerging evidence indicates that in addition to the well-recognized canonical antimicrobial function of MPO, it can directly or indirectly impact immune cells and tissue responses in homeostatic and disease states. Here, we highlight the emerging non-canonical functions of MPO, including its impact on neutrophil longevity, activation and trafficking in inflammation, its interactions with other immune cells, and how these interactions shape disease outcomes. We further discuss MPO interactions with barrier forming endothelial and epithelial cells, specialized cells of the central nervous system (CNS) and its involvement in cancer progression. Such diverse function and the MPO association with numerous inflammatory disorders make it an attractive target for therapies aimed at resolving inflammation and limiting inflammation-associated tissue damage. However, while considering MPO inhibition as a potential therapy, one must account for the diverse impact of MPO activity on various cellular compartments both in health and disease.

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Hypochlorous acid induced structural and conformational modifications in human DNA: A multi-spectroscopic study

Cited by 23 publications

References 34 publications

Electrolyzed hypochlorous acid water exhibits potent disinfectant activity against various viruses through irreversible protein aggregation

Electrolyzed hypochlorous acid water exhibits potent disinfectant activity against various viruses through irreversible protein aggregation

Влияние структурной организации нуклеиновых кислот на взаимодействие с гипохлоритом: АТФP, PolyA и ДНК

Non-Canonical Functions of Myeloperoxidase in Immune Regulation, Tissue Inflammation and Cancer

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