Hypobaric Hypoxia Aggravates Renal Injury by Inducing the Formation of Neutrophil Extracellular Traps through the NF-κB Signaling Pathway

Wei, Junyu; Hu, Miaoyue; Chen, Xiuqi; Wei, Jinshuang; Chen, Jie; Qin, Xuan-Kai; Lei, Feng-Ying; Zou, Jia-Sen; Zhu, Shi-qun; Qin, Yue

doi:10.1007/s11596-023-2744-3

Cited by 2 publications

(1 citation statement)

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“…Subsequently, the sections were incubated in a blocking solution for 30 min and then kept overnight at 4°C with a primary antibody, NF-κB-p65 (1:300, sc-8008, Santa Cruz Biotechnology) diluted in PBS. Next, the slices were incubated with the fluorescent-dye conjugated second antibody, Alexa Fluor 594 (1:400, Yeasen 33212ES60) for 50 min, and stained with DAPI for 5 min, and then mounted with anti-fade fluorescence mounting medium ( Wei et al, 2023 ). The fluorescent images were acquired by a fluorescence microscope (Leica GER) at ×20 magnification and the positive area was calculated semi-quantitatively by imajeJ.…”

Section: Methodsmentioning

confidence: 99%

Ginsenosides ameliorates high altitude-induced hypoxia injury in lung and kidney tissues by regulating PHD2/HIF-1α/EPO signaling pathway

Ji,

Zhang,

Mingyao

et al. 2024

Front. Pharmacol.

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Background: The primary constituent of ginseng, known as ginsenosides (GS), has been scientifically demonstrated to possess anti-fatigue, anti-hypoxia, anti-inflammatory, and antioxidant properties. However, the effect and mechanisms of GS on tissue injury induced by high-altitude hypoxia still remain unclear.Aim of the study: This study aims to investigate the protective effect of GS on a high-altitude hypoxia model and explore its mechanism.Materials and methods: Sprague-Dawley rats were placed in a high-altitude simulation chamber for 48 h (equivalent to an altitude of 6,000 m) to establish a high-altitude hypoxia model. We assessed the anti-hypoxic efficacy of GS through blood gas analysis, complete blood count, and hemorheology analysis. We used H&E and hypoxia probe assays to evaluate the protective effect of GS on organ ischemia-induced injury. Further, we used ELISA and qPCR analysis to detect the levels of inflammatory factors and oxidative stress markers. Immunohistochemistry and immunofluorescence staining were performed to determinate protein expression of hypoxia inducible factor 1-alpha (HIF-1α), erythropoietin (EPO), and prolyl hydroxylase 2 (PHD2).Results: In the survival experiment of anoxic mice, 100 mg/kg of GS had the best anti-anoxic effect. GS slowed down the weight loss rate of rats in hypoxic environment. In the fluorescence detection of hypoxia, GS reduced the fluorescence signal value of lung and kidney tissue and alleviated the hypoxia state of tissue. Meanwhile GS improved blood biochemical and hematological parameters. We also observed that GS treatment significantly decreased oxidative stress damage in lung and kidney tissues. Further, the levels of inflammatory factors, IL-1β, IL-6, and TNF-α were reduced by GS. Finally, GS regulated the PHD2/HIF-1α/EPO signaling pathway to improve blood viscosity and tissue hyperemia damage.Conclusion: GS could alleviate high-altitude induced lung and kidney damage by reducing the level of inflammation and oxidative stress, improving blood circulation through the PHD2/HIF-1α/EPO pathway.

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Section: Methodsmentioning

confidence: 99%