Hyphomycetes from aquatic habitats in Southern China: Species of Curvularia (Pleosporaceae) and Phragmocephala (Melannomataceae)

Abstract

Aquatic hyphomycetes are a diverse, polyphyletic group of asexually reproducing fungi involved in the decomposition of litter in freshwater ecosystems. Curvularia eragrostidis, C. verruculosa and Phragmocephala atra were identified from submerged wood collected from freshwater streams in Yunnan Province, Southwestern China. They were characterised based on morphology and LSU, ITS and SSU sequence data. Phylogenetic analysis of LSU sequences placed the isolates within the order Pleosporales. Curvularia eragrostidis and C. verruculosa are reported from freshwater habitats for the first time. An epitype is designated for Curvularia verruculosa. This is the first phylogenetic placement of the genus Phragmocephala in the family Melanommataceae in Dothideomycetes, providing new DNA sequence data. A new species, Phragmocephala garethjonesii is introduced based on DNA sequence data and morphology. Descriptions and illustrations are provided for the species with notes on their taxonomy and phylogeny.

Two new species of Helicascus (Morosphaeriaceae) from submerged wood in northern Thailand

“…The final volume of the PCR reaction was 25 μL and contained 12.5 μL of 2×Power Taq PCR MasterMix (a premix and ready to use solution, including 0.1 Units/μl Taq DNA Polymerase, 500 μm dNTP Mixture each (dATP, dCTP, dGTP, dTTP), 20 mM Tris-HCl pH 8.3, 100MmKCl, 3 mMMgCl 2 , stabilizer and enhancer), 1 μL of each primer (10 μM), 1 μL genomic DNA extract and 9.5 μL deionised water. The PCR thermal cycles for the amplification of the gene regions were as described in Su et al (2015). PCR products were purified using minicolumns, purification resin and buffer according to the manufacturer's protocols (Amershamproduct code: 27-9602-01).…”

Curvularia microspora sp. nov. associated with leaf diseases of Hippeastrum striatum in China

“…the production of sympodial conidiophores with tretic, terminal and intercalary conidiogenous cells and elongate, transversely septate conidia with a dark basal scar (Boedijn 1933). Phylogenetic analyses compared the DNA sequence from four phylogenetic markers with related species in the trifolii -clade: Curvularia akaii , C. borreriae , C. gladioli , C. gaudauskasii , C. heteropogonis , C. pallescens and C. trifolii (Figure 1, Manamgoda et al 2012, 2015, Madrid et al 2014, Jeong et al 2015, Su et al 2015). These taxa are morphologically similar in producing a strongly protruding hilum (Madrid et al 2014).…”

Pseudobactrodesmium (Dactylosporaceae, Eurotiomycetes, Fungi) a Novel Lignicolous Genus

“…The amplifications were carried out in a 25 µL reaction volume containing 9.5 µL ddH 2 O, 12.5 µL 2 × PCR Master Mix, 1 µL DNA template, 1 µL each primer (10 µM). The PCR thermal cycles for the amplification of the gene regions followed the methods in Jeewon et al (2004); Réblová et al (2011), andSu et al (2015). PCR products were checked on 1% agarose electrophoresis gels stained with Gel Red.…”