Hyphomycetes Celuloliticos en Suelos De Bosques De Nothofagus, Tierra Del Fuego

Mártínez, A E; Chiocchio, Viviana M.; Godeas, Alicia Margarita

doi:10.4067/s0717-66432001000200003

Cited by 10 publications

(7 citation statements)

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“…The genera isolated in this study have been reported by other authors for their cellulolytic activity, e.g. , Pseudomonas ( Shastry and Prasad, 2002 ) Streptomyces ( Gaitán and Perez, 2007 ; Grigorevski de Lima et al , 2005 ; Gutiérrez et al , 2008 ), Rhodococcus and Penicillium ( Villalba et al , 2004 ), and Mortierella ( MartÃnez et al , 2001 ; Valenzuela et al , 2001 ).…”

Section: Discussionsupporting

confidence: 66%

Assessment of cellulolytic microorganisms in soils of Nevados Park, Colombia

Avellaneda-Torres¹,

Pulido²,

Rojas³

2014

Braz. J. Microbiol.

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A systematized survey was conducted to find soil-borne microbes that degrade cellulose in soils from unique ecosystems, such as the Superpáramo, Páramo, and the High Andean Forest in the Nevados National Natural Park (NNNP), Colombia. These high mountain ecosystems represent extreme environments, such as high levels of solar radiation, low atmospheric pressure, and extreme daily changes in temperature. Cellulolytic activity of the microorganisms was evaluated using qualitative tests, such as growth in selective media followed by staining with congo red and iodine, and quantitative tests to determine the activity of endoglucanase, β-glucosidase, exoglucanase, and total cellulase. Microorganisms were identified using molecular markers, such as the 16S rRNA gene for bacteria and the internal transcribed spacer region (ITS) of ribosomal DNA for fungi. Multivariate statistical analysis (MVA) was used to select microorganisms with high cellulolytic capacity. A total of 108 microorganisms were isolated from the soils and, in general, the enzymatic activities of fungi were higher than those of bacteria. Our results also found that none of the organisms studied were able to degrade all the components of the cellulose and it is therefore suggested that a combination of bacteria and/or fungi with various enzymatic activities be used to obtain high total cellulolytic activity. This study gives an overview of the potential microorganism that could be used for cellulose degradation in various biotechnological applications and for sustainable agricultural waste treatment.

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Section: Discussionsupporting

confidence: 66%

Assessment of cellulolytic microorganisms in soils of Nevados Park, Colombia

Avellaneda-Torres¹,

Pulido²,

Rojas³

2014

Braz. J. Microbiol.

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“…Agar block (6 mm in diameter) from five days old fungal colony grown on MEA plates was cut and inoculated in the center of the plates. Inoculated plates were incubated at 28 ± 2 °C for 4 days to estimate the growth extension of the colony (Guigón-López et al, 2010;Andrino, Morte, & Honrubia, 2011) and the degradation of carbon substrate supplemented through the procedure of Mangelli & Forchiassin (1999), calculated as the Cellulolytic Efficiency Index (CEI) according to Martínez, Chiocchio, & Godeas (2001). All analyzes were performed in duplicate.…”

Section: Methodsmentioning

confidence: 99%

Cellulolytic ability of a promising Irpex lacteus (Basidiomycota: Polyporales) strain from the subtropical rainforest of Misiones province, Argentina

Giorgio

Villalba

Robledo

et al. 2018

RBT

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The cellulolytic activity of fungi growing in the subtropical rainforest of Misiones (Argentina) represents a challenge in the technological development of the production of cellulosic bioethanol in the region using native sources. These fungi are promising to obtain sustainable enzyme cocktails using their enzymes. Cellulolytic ability of 22 white-rot fungi isolated from the subtropical rainforest of Misiones-Argentina in agar medium with two types of cellulosic substrates, carboxy-methylcellulose or crystalline cellulose, were comparatively analyzed, and the activity of two cellulolytic enzymes was evaluated in liquid medium. Although all isolates were able to grow and degrade both substrates in agar medium, and to produce total cellulase Filter paper (FPase) and endo-β-1,4-glucanase (EG) activities in broth, the isolate Irpex sp. LBM 034 showed the greatest enzymatic levels (FPase, 65.45 U L-1; EG, 221.21 U L-1). Therefore, the ITS sequence of this fungus was sequenced and analyzed through a phylogenetic analysis. These results indicate that the isolate LBM 034, corresponding to Irpex lacteus, has a promising cellulolytic ability and enzymes such as EG useful in sustainable saccharification of cellulosic materials in the region.

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“…5,19,20,33 Humicolopsis cephalosporioides Cabral & Marchand (anamorph Ascomycota, Insertae Sedis) was identified as one of the soil fungi with high cellulolytic activity in Tierra del Fuego (Argentina). 10,17,18 However there is no information about the ability of H. cephalosporioides to use other organic substrates, its enzyme systems and its role in nutrient cycling. Recently, we isolated four strains of H. cephalosporioides from soils of N. pumilio harvested by shelter wood-cut management.…”

Section: Datos Preliminares Sobre El Crecimiento Y La Capacidad Enzim...mentioning

confidence: 99%