Hyphenation of ionic liquid albumin glassy carbon biosensor or protein label-free sensor with differential pulse stripping voltammetry for interaction studies of human serum albumin with fenoprofen enantiomers

El‐Hady, Deia Abd; Youssef, Adel F.

doi:10.1016/j.aca.2013.02.018

Cited by 21 publications

(23 citation statements)

References 27 publications

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“…This suggested electrochemical mechanism opened a new venue for diverse applications of proposed biosensor for the determination of small molecules. Therefore, the current results indicated that ionic liquid BMIMPF 6 is considered a suitable mediator and promoter for the electrochemical activity of protein on GCE [19]. Therefore, it could be considered that BMIMPF 6 enhanced for the stability and electrochemical activity of HSA on GCE.…”

Section: Ilagc Biosensor and Retinol Electrochemical Behaviormentioning

confidence: 77%

“…The cleaned electrodes were dried in a room temperature. The ionic liquid human serum albumin modified GCE was simply prepared by following the procedure described in our previous work [19]. The HSA solution was mixed with BMIMPF 6 and then the mixture was dripped on the glassy carbon electrode surface and dried by passing very slow rate of hot air for 10.0 min to form a stable gel-like film.…”

Section: Preparation Of the Electrochemical Biosensormentioning

confidence: 99%

“…The value of binding constant for the interaction of retinol with HSA was determined by using the equation mentioned in our published work [19]; the binding number (m) and binding constant (K) of HSA-m retinol were calculated.…”

Section: Determination Of the Binding Constant Between Retinol And Hsamentioning

confidence: 99%

“…The covalent binding technique was often used for protein immobilization [17,18], but the process of covalent immobilization was somewhat complex and might induce protein denaturation. Recently, we have fabricated an ionic liquid albumin glassy carbon (ILAGC) biosensor and used it for the chiral discrimination of fenoprofen enantiomers [19]. In this work, ILAGC exhibits a stable biosensor for electrochemical measurements due to the moderate hydrophobicity of IL and its possibility to adsorb on the electrode substrate or attractive interactions with protein.…”

Section: Introductionmentioning

confidence: 99%

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Green Ionic Liquid Albumin Glassy Carbon Biosensor (ILAGC) for On-line Binding Studies of Human Serum Albumin with Retinol

Albishri¹

2013

J Anal Bioanal Tech

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A new biosensor composed of 1-butyl-3-methylimidazolium hexafluorophosphates (BMIMPF 6 )-human serum albumin (HSA) film coated on glassy carbon electrode (GCE) was produced. In the current work, the conjugation of ionic liquid (IL) with HSA improved the stability and binding affinity of protein onto GCE. A rapid and reliable voltammetric method for the on-line protein binding studies of retinol with HSA was developed by hyphenating ionic liquid albumin glassy carbon (ILAGC) biosensor with differential pulse anodic stripping voltammetry under physiological conditions. Detection limit and quantitation limit of 4.5 and 15.0 nmol L -1 were achieved, respectively. This gave us the opportunity to study the high binding constant between retinol and HSA by their detection in nanomolars. The electrochemical behavior of retinol onto ILAGC biosensor was monitored by cyclic voltammetry. The surface coverage of retinol on the proposed biosensor was calculated by double potential-step chronocoulometry. The binding constant of retinol with HSA was estimated to be 1.30×105 L mol -1 giving acceptable precision (SD ≤ 0.02) and good agreement with literature values. The proposed electrochemical biosensor opened a new venue for cheap, green and rapid on-line binding studies of small molecules with protein. As well, our proposed method could use to study either high or low binding affinities.

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Section: Ilagc Biosensor and Retinol Electrochemical Behaviormentioning

confidence: 77%

Section: Preparation Of the Electrochemical Biosensormentioning

confidence: 99%

Section: Determination Of the Binding Constant Between Retinol And Hsamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Green Ionic Liquid Albumin Glassy Carbon Biosensor (ILAGC) for On-line Binding Studies of Human Serum Albumin with Retinol

Albishri¹

2013

J Anal Bioanal Tech

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“…Recently, ionic liquids (ILs) were used for the modification of carbon electrodes because of their properties, including high/moderate conductivity, negligible vapor pressure, good thermal stability and ability to form micelles with the variable possibility of interactions by electrostatic, hydrophobic, π-π and hydrogen bonding [7][8]. 4 The electrode material consisting of IL acquired the same properties of ILs.…”

Section: Introductionmentioning

confidence: 99%

Protein/ionic liquid/glassy carbon sensors following analyte focusing by ionic liquid micelle collapse for simultaneous determination of water soluble vitamins in plasma matrices

El‐Hady

Albishri

2015

Talanta

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Designing Powerful Biindole‐Based Inherently Chiral Selectors: Enhancing Enantiodiscrimination by Core Functionalization with Additional Coordination Elements

Grecchi,

Bonetti,

Emanuele

et al. 2024

Chemistry A European J

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Among inherently chiral selectors of axial stereogenicity, usually resulting in very good enantiodiscrimination performances, the biindole‐based family has the additional advantage of very easy functionalization of the two nitrogen atoms with a variety of substituents with desirable properties. Aiming to evaluate the possibility of exploiting such feature to enhance the enantiodiscrimination ability of the archetype structure, a series of three inherently chiral monomers were designed and synthesized, characterised by a 2,2’‐biindole atropisomeric core conjugated to bithiophene wings enabling fast and regular electrooligomerization, and functionalised at the nitrogen atoms with an ethyl, a methoxyethyl, or a hydroxyethyl substituent. Nitrogen alkylation was also exploited to obtain for the first time the chemical resolution of the biindole selectors without employing chiral HPLC. The enantiodiscrimination ability of the selector series was comparatively evaluated in proof‐of‐concept chiral voltammetry experiments with a “benchmark” chiral ferrocenyl probe as well as with chiral non‐steroidal anti‐inflammatory drugs naproxen and ketoprofen. The large enantiomer potential differences for all probes increased in the ethyl < methoxyethyl ≪ hydroxyethyl sequence of selector substituents, supporting our assumption on the beneficial role of an additional coordination element. The powerful hydroxyethyl selector was also applied to ketoprofen in a commercial drug matrix.

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Hyphenation of ionic liquid albumin glassy carbon biosensor or protein label-free sensor with differential pulse stripping voltammetry for interaction studies of human serum albumin with fenoprofen enantiomers

Cited by 21 publications

References 27 publications

Green Ionic Liquid Albumin Glassy Carbon Biosensor (ILAGC) for On-line Binding Studies of Human Serum Albumin with Retinol

Green Ionic Liquid Albumin Glassy Carbon Biosensor (ILAGC) for On-line Binding Studies of Human Serum Albumin with Retinol

Protein/ionic liquid/glassy carbon sensors following analyte focusing by ionic liquid micelle collapse for simultaneous determination of water soluble vitamins in plasma matrices

Designing Powerful Biindole‐Based Inherently Chiral Selectors: Enhancing Enantiodiscrimination by Core Functionalization with Additional Coordination Elements

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