Hyphenated Analytical Methods in Determination of Biologically Active Compounds in Hen's Eggs

Walczak, Justyna; Bocian, Szymon; Trziszka, Tadeusz; Buszewski, Bogusław

doi:10.1080/10408347.2015.1023428

Cited by 7 publications

(5 citation statements)

References 117 publications

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“…In addition, eggs are considered nutraceuticals, i.e. natural food with health-promoting and therapeutic functions (Nain et al, 2012), and 'designer eggs' may contain several times more biologically active substances (Trziszka et al, 2013;Walczak et al, 2016). An undoubted advantage of eggs is that their composition can be modulated relatively easily, in the case of both nutrients and elements (Szymanek et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Comparison of the quality of cage and organic eggs available in retail and their content of selected macroelements

Banaszewska

Biesiada–Drzazga

Marciniuk

et al. 2020

Acta Sci Pol Technol Aliment

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Background. The chicken egg is a food product with a rich content of nutrients, such as proteins, vitamins, lipids, and minerals with high bioavailability. Furthermore, eggs are easy to prepare and a relatively inexpensive component of the human diet. The aim of this study was to compare the quality of eggs from an organic and a conventional farm and their content of Na and K. Material and methods. The research material consisted of eggs from laying hens reared in two different systems-organic (according to standards for organic farming and with access to a chicken run) and cage. Forty eggs from each group were analysed. Egg quality traits were divided into destructive and non-destructive. In addition, potassium (K) and sodium (Na) contents were determined in the whole egg, yolk and albumen. Results. The research results indicated slightly better quality of eggs from organic farming compared to eggs from cages in the case of most physical properties. The data clearly show that the content of sodium and potassium in the albumen, yolk and whole egg was higher in the eggs of chickens raised organically compared to the eggs of chickens reared in cages (P ≤ 0.05). Conclusions. The research results indicate a slightly better quality of eggs from the organic farm compared to eggs from cages in the case of most physical properties, as well as the content of macro-elements. Eggs in both systems are produced following scientific management practices. There are many myths among consumers regarding the nutritional quality of eggs produced in different systems. This information can be useful for raising awareness among consumers selecting eggs.

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Section: Introductionmentioning

confidence: 99%

Comparison of the quality of cage and organic eggs available in retail and their content of selected macroelements

Banaszewska

Biesiada–Drzazga

Marciniuk

et al. 2020

Acta Sci Pol Technol Aliment

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“…Earlier results gained by two-dimensional electrophoresis suggest that egg white protein abundances could differ importantly between both chicken breeds and populations. 7 Since the egg white contains large amounts of proteins involved in antimicrobial defense, 8 such variation could potentially affect egg quality, biosafety, and chicken embryo development.…”

Section: ■ Introductionmentioning

confidence: 99%

Domestic Fowl Breed Variation in Egg White Protein Expression: Application of Proteomics and Transcriptomics

Bílková

Świderská

Zita

et al. 2018

J. Agric. Food Chem.

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Avian egg white is essential for protecting and nourishing bird embryos during their development. Being produced in the female magnum, variability in hen oviduct gene expression may affect egg white composition in domestic chickens. Since traditional poultry breeds may represent a source of variation, in the present study we describe the egg white proteome (mass spectrometry) and corresponding magnum transcriptome (high-throughput sequencing) for 20 hens from five domestic fowl breeds (large breeds: Araucana, Czech golden pencilled, Minorca; and small breeds: Booted bantam, Rosecomb bantam). In total, we identified 189 egg white proteins and 16391 magnum-expressed genes. The majority of egg white protein content comprised proteins with an antimicrobial function. Despite general similarity, Between-class Principal Component Analysis revealed significant breed-specific variability in protein abundances, differentiating especially small and large breeds. Though we found strong association between magnum mRNA expression and egg white protein abundance across genes, coinertia analysis revealed no transcriptome/proteome costructure at the individual level. Our study is the first to show variation in protein abundances in egg white across chicken breeds with potential effects on egg quality, biosafety, and chick development. The observed interindividual variation probably results from post-transcriptional regulation creating a discrepancy between proteomic and transcriptomic data. KEYWORDS: antimicrobial peptides, avian oviduct transcriptome, bird albumen, chicken breed, egg white proteome

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“…Two proteins responsible for the variance between fluids are proteins from the serpin family—OVAL (ovalbumin) and OVALY (ovalbumin-related protein Y). Both proteins are also present in egg white which is predominantly composed of proteins that exhibit antimicrobial activity 75 , 76 , which was confirmed for OVAL 77 , 78 . There is also higher abundance of RBP (riboflavin-binding protein), whose precursor was already found in laying hens, where its occurrence was primarily associated with the transfer of vitamin B to the egg 79 .…”

Section: Discussionmentioning

confidence: 58%

Proteomic analysis reveals dynamic changes in cloacal fluid composition during the reproductive season in a sexually promiscuous passerine

Míčková,

Jelínek,

Tomášek

et al. 2024

Sci Rep

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Cryptic female choice (CFC) is a component of postcopulatory sexual selection that allows females to influence the fertilization success of sperm from different males. While its precise mechanisms remain unclear, they may involve the influence of the protein composition of the female reproductive fluids on sperm functionality. This study maps the protein composition of the cloacal fluid across different phases of female reproductive cycle in a sexually promiscuous passerine, the barn swallow. Similar to mammals, the protein composition in the female reproductive tract differed between receptive (when females copulate) and nonreceptive phases. With the change in the protein background, the enriched gene ontology terms also shifted. Within the receptive phase, distinctions were observed between proteomes sampled just before and during egg laying. However, three proteins exhibited increased abundance during the entire receptive phase compared to nonreceptive phases. These proteins are candidates in cryptic female choice, as all of them can influence the functionality of sperm or sperm-egg interaction. Our study demonstrates dynamic changes in the cloacal environment throughout the avian breeding cycle, emphasizing the importance of considering these fluctuations in studies of cryptic female choice.

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Hyphenated Analytical Methods in Determination of Biologically Active Compounds in Hen's Eggs

Cited by 7 publications

References 117 publications

Comparison of the quality of cage and organic eggs available in retail and their content of selected macroelements

Comparison of the quality of cage and organic eggs available in retail and their content of selected macroelements

Domestic Fowl Breed Variation in Egg White Protein Expression: Application of Proteomics and Transcriptomics

Proteomic analysis reveals dynamic changes in cloacal fluid composition during the reproductive season in a sexually promiscuous passerine

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