Hyphal wall composition inApodachlyella completa

Synthesis in vitro of crystalline chitin by a solubilized enzyme from the cellulosic fungus Saprolegnia monoica

“…Biophysical or cytochemical studies have reveaIed that chitin is present in Leptomitales (Aronson & Lin, 1978;Bertke & Aronson, 1980) and in Achyh (Dietrich, 1975 ;CamposTakaki ef a/., 1982). Recently we demonstrated the presence of chitin in the cell wall of Saprulegnzi monoica by chemical extraction of the polymer, X-ray and electron diffraction patterns and infrared spectroscopy.…”

Hyphal Wall Composition of Mindeniella Spinospora and Araiospora Sp.

“…Walls of Apodachyla sp., L. lacteus and A. completa also contain, in addition to chitin, the ,B-glucans typical of Oomycetes. Cellulose fractions in these leptomitacean species, however, constitute less than 10% of wall weight (Lin et al, 1976;Aronson and Lin, 1978;Bertke and Aronson, 1980). Based on results of the present study with M. spinospora and Araiospora sp., along with a previous investigation of the rhipidiacean species, Sapromyces elongatus (Pao and Aronson, 1970) and a recent finding that walls of Sapromyces elongatus contain 28% cellulose (Bertke and Aronson, unpublished results), it appears that the rhipidiacean genera may be characterized by their unusually high cellulose contents as well as the absence ofchitin.…”

“…Although the absence of chitin in walls of these oomycetes is not unexpected, these observations become particularly significant when the wall compositions of these two rhipidacean genera are compared with previously investigated leptomitalean genera in the family Leptomitaceae. Studies ofhypha1wall composition in the leptomitacean species, Apodachlya sp., Leptomitus lacteus, and Apodachlyella completa, demonstrated 18%, 14% and 5% chitin, respectively (Lin et al, 1976;Aronson and Lin, 1978;Bertke and Aronson, 1980). Additionally, a paper chromatographic survey of walls of a comparatively large number of oomycetes reported by Vaziri-Tehrani and Dick (1980) has provided some qualitative confirmation for the presence of hexosamine in Leptomitaceae and its apparent absence in Rhipidiaceae.…”

“…genera lack chitin, as does Sapromyces, cell wall composition would be a definitive character distinguishing members of the two leptomitalean families (Aronson and Lin, 1978;Bertke and Aronson, 1980;Lin et al, 1976). This biochemical criterion takes on added significance since conventional morphological approaches to the systematics and phylogeny of leptomitalean fungi have resulted in disparate views (Dick, 1973;Emerson and Weston, 1967;Sparrow, 1976).…”

“…Mycelia, harvested by filtration, were washed free ofculture medium with chilled 0.1 Mtris-hydrochloride buffer, pH 7.5. Maintaining samples at 3-5 C, mycelia were given a preliminary homogenization in a Sorvall Omnimixer (Lin et al, 1976) followed by more extensive treatment in a Braun MSK cell homogenizer (Bertke and Aronson, 1980). Treatment times during the latter step were 2 min and 6 min, respectively, for Mindeniella and Araiospora.…”