Hyphal Growth in
            <i>Trichosporon asahii</i>
            Is Accelerated by the Addition of Magnesium

Aoki, Keita; Yamamoto, Kosuke; Ohkuma, Moriya; Sugita, Takashi; Tanaka, Naoto; Takashima, Masako

doi:10.1128/spectrum.04242-22

Cited by 5 publications

(3 citation statements)

References 68 publications

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“…We hypothesized that Hog1 in T. asahii controls the expression of genes related to hyphal formation. Hyphal formation in T. asahii is induced by magnesium 55 . In C. albicans , magnesium deficiency activates Hog1 56 .…”

Section: Discussionmentioning

confidence: 99%

Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii

Matsumoto,

Sugiyama,

Nagamachi

et al. 2023

Sci Rep

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Trichosporon asahii is an opportunistic pathogenic fungus that causes severe and sometimes fatal infections in immunocompromised patients. Hog1, a mitogen-activated protein kinase, regulates the stress resistance of some pathogenic fungi, however its role in T. asahii has not been investigated. Here, we demonstrated that the hog1 gene-deficient T. asahii mutant is sensitive to high temperature, cell membrane stress, oxidative stress, and antifungal drugs. Growth of the hog1 gene-deficient T. asahii mutant was delayed at 40 °C. The hog1 gene-deficient T. asahii mutant also exhibited sensitivity to sodium dodecyl sulfate, hydrogen peroxide, menadione, methyl methanesulfonate, UV exposure, and antifungal drugs such as amphotericin B under a glucose-rich condition. Under a glucose-restricted condition, the hog1 gene-deficient mutant exhibited sensitivity to NaCl and KCl. The virulence of the hog1 gene-deficient mutant against silkworms was attenuated. Moreover, the viability of the hog1 gene-deficient mutant decreased in the silkworm hemolymph. These phenotypes were restored by re-introducing the hog1 gene into the gene-deficient mutant. Our findings suggest that Hog1 plays a critical role in regulating cellular stress responses in T. asahii.

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Section: Discussionmentioning

confidence: 99%

Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii

Matsumoto,

Sugiyama,

Nagamachi

et al. 2023

Sci Rep

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“…Calcium and magnesium ions, as in other eukaryotic organisms, are essential for the growth of fungi and are involved in diverse functions [ 40 , 41 , 42 ]. We explored the correlations between the Ca/Mg ratio and the structure of the mycobiome communities in the two kinds of samples from Luohandu cave ( Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

Deconstructing the Dimensions of Mycobiome Fingerprints in Luohandu Cave, Guilin, Southern China

Man,

Xiang,

Cheng

et al. 2024

Microorganisms

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Subterranean karst caves are windows into the terrestrial subsurface to deconstruct the dimensions of mycobiome fingerprints. However, impeded by the constraints of remote locations, the inaccessibility of specimens and technical limitations, the mycobiome of subterranean karst caves has remained largely unknown. Weathered rock and sediment samples were collected from Luohandu cave (Guilin, Southern China) and subjected to Illumina Hiseq sequencing of ITS1 genes. A total of 267 known genera and 90 known orders in 15 phyla were revealed in the mycobiomes. Ascomycota dominated all samples, followed by Basidiomycota and Mortierellomycota. The sediments possessed the relatively highest alpha diversity and were significantly different from weathered rocks according to the diversity indices and richness metrics. Fifteen families and eight genera with significant differences were detected in the sediment samples. The Ca/Mg ratio appeared to significantly affect the structure of the mycobiome communities. Ascomycota appeared to exert a controlling influence on the mycobiome co-occurrence network of the sediments, while Ascomycota and Basidiomycota were found to be the main phyla in the mycobiome co-occurrence network of weathered rocks. Our results provide a more comprehensive dimension to the mycobiome fingerprints of Luohandu cave and a new window into the mycobiome communities and the ecology of subterranean karst cave ecosystems.

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“…Therefore, gaining insight into the infection mechanisms and drug-resistant systems of T. asahii is crucial. T. asahii JCM2466 (type strain CBS2479, Riken BioResource Research Center) is widely used in research (Aoki et al 2023; Kurakado et al 2020). The genome information of T. asahii JCM2466 (CBS2479) has been published (Yang et al 2012).…”

Section: Introductionmentioning

confidence: 99%

A gene-targeting system in Trichosporon asahii JCM2466 by Agrobacterium tumefaciens-mediated transformation

Matsumoto,

Nakayama,

Shimizu

et al. 2024

Preprint

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Trichosporon asahii is a pathogenic fungus that causes severe, deep-seated fungal infections in neutropenic patients. The complete genome information of T. asahii JCM2466 (CBS2479), a standard type strain widely used in research, is publicly available, but a gene-targeting system for T. asahii JCM2466 has not yet been developed. Here we generated gene-deficient mutants of T. asahii JCM2466 by Agrobacterium tumefaciens-mediated transformation (ATMT). A gene-deficient mutant lacking the ku70 gene, which encodes the Ku70 protein involved in non-homologous end-joining repair of DNA double-strand breaks, was generated by ATMT. The ku70 gene-deficient mutant exhibited higher gene-targeting efficiency than the wild-type strain for generating a mutant lacking the cnb1 gene, which encodes the beta-subunit of calcineurin. In the ku70 gene-deficient mutant used as a parent strain, cnb1 gene-deficient mutants were generated by ATMT. The cnb1 gene-deficient mutants exhibited sensitivities to high temperature and several stress-inducing compounds. These results suggest that the combination of the ku70 gene-deficient mutant and ATMT is a useful method for generating gene-deficient mutants of T. asahii JCM2466.

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Hyphal Growth in Trichosporon asahii Is Accelerated by the Addition of Magnesium

Abstract: Understanding the mechanism underlying fungal dimorphism is crucial to discern its invasion into human cells. Invasion is caused by the hyphal form rather than the yeast form; therefore, it is important to understand the mechanism of transition from the yeast to hyphal form.

Cited by 5 publications

References 68 publications

Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii

Hog1-mediated stress tolerance in the pathogenic fungus Trichosporon asahii

Deconstructing the Dimensions of Mycobiome Fingerprints in Luohandu Cave, Guilin, Southern China

A gene-targeting system in Trichosporon asahii JCM2466 by Agrobacterium tumefaciens-mediated transformation

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