Hypervariable antigenic region 1 of classical swine fever virus E2 protein impacts antibody neutralization

Liao, Xun; Wang, Zuohuan; Cao, Tong; Tong, Chao; Geng, Shichao; Gu, Yunqing; Zhou, Yingshan; Li, Xiaoliang; Fang, Weihuan

doi:10.1016/j.vaccine.2016.06.007

Cited by 12 publications

(5 citation statements)

References 36 publications

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“…The R value between CSFV Alfort 187 and CSFV Diepholz was 10.8 (representing a 9.25-fold difference), indicating significant antigenic differences even between strains belonging to the same pestivirus species. In fact, it is well known that variations of E2 in CSFV field strains may influence the reaction patterns and cross-neutralization with sera from heterologous strains (Liao et al, 2016). The highest R values (28.8 and 30, representing 3.3fold and 3.5-fold differences) were observed, as expected, between BVDV-1h and BVDV-1b and, interestingly, between the ovine pestivirus and CSFV Diepholz, indicating the close serological relatedness between these virus pairs (Figures S1 and S2).…”

Section: Re Sults and Discussionmentioning

confidence: 99%

Serological relationship between a novel ovine pestivirus and classical swine fever virus

Casciari

Sozzi

Bazzucchi

et al. 2020

Transbound Emerg Dis

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The genus Pestivirus comprises globally distributed members of the family Flaviviridae, which cause severe losses in livestock. The most common species of the genus are bovine viral diarrhoea virus type 1 (BVDV‐1) and type 2 (BVDV‐2), classical swine fever virus (CSFV) and border disease virus (BDV). Recently, a novel ovine pestivirus was repeatedly detected in aborted lamb foetuses on a farm located in the Brescia Province (Italy). Complete genome characterization of this isolate showed that it was highly divergent from known pestivirus species and that it was genetically closely related to CSFV. The aim of this study was to determine the serological relatedness between the identified novel pestivirus and BVDV, BDV and CSFV selected strains for which homologous serum was available, by antigenic characterization performed using cross‐neutralization assays. The serological relatedness was expressed as the coefficient of antigenic similarity (R). Both field and specific antisera raised against the ovine pestivirus neutralized the CSFV reference strain Diepholz with titres significantly higher than those specific for the BDV and BVDV strains. Furthermore, the calculated R values clearly indicated that the novel ovine pestivirus is antigenically more related to CSFV than to ruminant pestiviruses, in agreement with the results of the genomic analysis. This would have severe consequences on CSFV serology in the event of a switch to porcine hosts with implications for CSFV surveillance and porcine health management.

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Section: Re Sults and Discussionmentioning

confidence: 99%

Serological relationship between a novel ovine pestivirus and classical swine fever virus

Casciari

Sozzi

Bazzucchi

et al. 2020

Transbound Emerg Dis

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“…However, the present analysis indicated antigenic differences between CSFV strain Alfort/187 and Paderborn, whereas both viruses belong to one pestivirus species ( Table S1 ). It has already been mentioned that differences in the E2 protein sequence can influence cross-neutralization with sera from heterologous CSFV strains [ 45 ]. The dendrogram showed that the pestivirus strain Aydin and TSV are antigenically more closely related to CSFV (averaged R value for the Aydin pestivirus and the three CSFV strains = 5.4; averaged R value for TSV and the three CSFV strains = 3.9) than to BDV (averaged R value for Aydin pestivirus and the two BDV strains = 2.1; averaged R value for TSV and the two BDV strains = 2.1), respectively ( Table S2 ).…”

Section: Resultsmentioning

confidence: 99%

Comparative Analysis of Tunisian Sheep-like Virus, Bungowannah Virus and Border Disease Virus Infection in the Porcine Host

Meyer

Postel

Wiedemann

et al. 2021

Viruses

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Apart from the established pestivirus species Pestivirus A to Pestivirus K novel species emerged. Pigs represent not only hosts for porcine pestiviruses, but are also susceptible to bovine viral diarrhea virus, border disease virus (BDV) and other ruminant pestiviruses. The present study focused on the characterization of the ovine Tunisian sheep-like virus (TSV) as well as Bungowannah virus (BuPV) and BDV strain Frijters, which were isolated from pigs. For this purpose, we performed genetic characterization based on complete coding sequences, studies on virus replication in cell culture and in domestic pigs, and cross-neutralization assays using experimentally derived sera. TSV forms a distinct phylogenetic group more closely related to Pestivirus C (classical swine fever virus, CSFV) than to Pestivirus D (BDV). In contrast to BDV and BuPV, TSV replicates by far more efficiently on ovine than on porcine cells. Nevertheless, pigs were susceptible to TSV. As a consequence of close antigenic relatedness of TSV to CSFV, cross-reactivity was detected in CSFV-specific antibody assays. In conclusion, TSV is genetically closely related to CSFV and can replicate in domestic pigs. Due to close antigenic relatedness, field infections of pigs with TSV and other ruminant pestiviruses can interfere with serological diagnosis of classical swine fever.

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“…Previous studies demonstrated that residue E713 is a major determinant of antigenic variation of CSFV field strains. The G713E substitution enhanced the recognition of recombinant vaccine strains and mutated E2 protein belonging to genotype 1.1 by mAbs [ 33 , 50 ] or swine serum against CSFV genotype 2.1 [ 51 ]. This present study is the first report demonstrating that residue E713 represents an important antigenic determinant residue of a common conformational epitope shared by both CSFV and BDV.…”

Section: Discussionmentioning

confidence: 99%

Identification of a Common Conformational Epitope on the Glycoprotein E2 of Classical Swine Fever Virus and Border Disease Virus

Huang

Meyer²,

Postel³

et al. 2021

Viruses

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Classical swine fever virus (CSFV) shares high structural and antigenic homology with bovine viral diarrhea virus (BVDV) and border disease virus (BDV). Because all three viruses can infect swine and elicit cross-reactive antibodies, it is necessary to differentiate among them with regard to serological diagnosis of classical swine fever. To understand the mechanism of cross-reactivity, it is important to define common or specific epitopes of these viruses. For this purpose, epitope mapping of six monoclonal antibodies (mAbs) was performed using recombinant expressed antigenic domains of CSFV and BDV E2 proteins. One CSFV-specific conformational epitope and one CSFV and BDV common epitope within domain B/C of E2 were identified. Site-directed mutagenesis confirmed that residues G725 and V738/I738 of the CSFV-specific epitope and P709/L709 and E713 of the second epitope are important for mAbs binding. Infection of CSFV in porcine cells was significantly reduced after pre-incubation of the cells with the domain B/C of E2 or after pre-incubation of CSFV with the mAbs detecting domain B/C. 3D structural modeling suggested that both epitopes are exposed on the surface of E2. Based on this, the identified epitopes represent a potential target for virus neutralization and might be involved in the early steps of CSFV infection.

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Hypervariable antigenic region 1 of classical swine fever virus E2 protein impacts antibody neutralization

Cited by 12 publications

References 36 publications

Serological relationship between a novel ovine pestivirus and classical swine fever virus

Serological relationship between a novel ovine pestivirus and classical swine fever virus

Comparative Analysis of Tunisian Sheep-like Virus, Bungowannah Virus and Border Disease Virus Infection in the Porcine Host

Identification of a Common Conformational Epitope on the Glycoprotein E2 of Classical Swine Fever Virus and Border Disease Virus

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