Hypertrophy of Adipose Tissues in Quail Embryos by in ovo Injection of All-Trans Retinoic Acid

Kim, Dong Hwan; Lee, Joonbum; Kim, Sanggu; Lillehoj, Hyun S.; Lee, Kichoon

doi:10.3389/fphys.2021.681562

Cited by 6 publications

(6 citation statements)

References 35 publications

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“…In chickens, our recent studies showed that atRA promoted lipid accumulation in primary preadipocytes ( Kim D.-H. et al, 2020 ). In addition, in ovo injection of atRA in quails resulted in increased fat pad weight with adipocyte hypotrophy ( Kim et al, 2021a ). These data suggest pro-adipogenic activity of atRA in preadipocyte and adipose tissues in vitro and in vivo .…”

Section: Discussionmentioning

confidence: 99%

“…These precursor cells have abilities to be differentiated into myocytes with the expression of myogenic transcriptional factors ( Rosen and MacDougald 2006 ; Rodeheffer et al, 2008 ). Especially, Pparγ has been served as a well-known indicator for adipogenic differentiation in mammals ( Tamori et al, 2002 ; Liang et al, 2016 ) and avian species ( Kim et al, 2020a , Kim et al, 2020b , Kim et al, 2021a , Kim et al, 2021b ; Lee et al, 2021 ). In addition, over-expression or activation of Pparγ can convert myogenic cells to adipocytes ( Hu et al, 1995 ; Teboul et al, 1995 ).…”

Section: Introductionmentioning

confidence: 99%

“…In various avian cell types, chicken embryonic fibroblasts which are isolated at embryonic day (E) 5, stromal vascular cells isolated from fat tissues of chicken embryos, and a DF-1 chicken embryonic fibroblast cell line can be induced to adipogenic cells in the culture condition supplementing atRA ( Kim D.-H. et al, 2020 ; Lee et al, 2021 ). Furthermore, direct in ovo injection of atRA increased adipose weight and size during embryonic development ( Kim et al, 2021a ). Although atRA has positive regulatory effects on adipogenic differentiation of various avian cell types, it has not been specifically investigated whether atRA can induce transdifferentiation of myoblasts into adipocytes in poultry.…”

Section: Introductionmentioning

confidence: 99%

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Transdifferentiation of Myoblasts Into Adipocytes by All-Trans-Retinoic Acid in Avian

Kim

Lee

Suh

et al. 2022

Front. Cell Dev. Biol.

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Increased adipogenesis in muscle tissues is related to metabolic syndromes and muscle weakness in humans and improvement of meat quality in animal production. With growing evidence for pro-adipogenic functions of all-trans-retinoic acid (atRA), the current study investigated whether atRA can transdifferentiate myoblasts into adipocytes using a quail myogenic cell line (QM7) and avian primary myoblasts. atRA increased cytoplasmic lipid droplet accumulation and mRNA expression for adipogenic genes in these cells. An acute induction of Pparγ expression by atRA under cycloheximide treatment indicated a direct regulation of Pparγ by atRA. In addition, the induction of Pparγ expression was mediated by retinoic acid receptors . At high levels of Pparγ by atRA, BADGE, an antagonist of Pparγ, inhibited, and rosiglitazone, an agonist of Pparγ, further enhanced atRA-induced transdifferentiation. However, at very low levels of Pparγ in the absence of atRA treatment, rosiglitazone could not induce transdifferentiation of avian myoblasts. These data suggest that the induction of Pparγ expression by atRA is an essential molecular event in myoblasts for atRA-induced transdifferentiation into adipocytes. Based on our findings, atRA can be a new transdifferentiation factor of myoblasts to adipocytes, providing a potential nutrient to enhance marbling in poultry.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transdifferentiation of Myoblasts Into Adipocytes by All-Trans-Retinoic Acid in Avian

Kim

Lee

Suh

et al. 2022

Front. Cell Dev. Biol.

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“…The in ovo injection of medical plant extracts (1% solution, e5, air cell) such as garlic, ginger, oregano, or cinnamon has been shown to influence the time of sexual maturity and the quality of early eggs laid by Japanese quails ( 197 ). The importance of all-trans retinoic acid, a metabolite of vitamin A in adipose tissue development has been emphasized by its in ovo injection (300 nM) on e9 into quail embryos ( 198 ). Additionally, in ovo injection of this metabolite increased mRNA expression of adipogenic markers and decreased expression of preadipocyte markers, suggesting the promotion of adipocyte differentiation and decrease population of preadipocytes ( 198 ).…”

Section: In Ovo Effects In Other Avian Speciesmentioning

confidence: 99%

Physiological effects of in ovo delivery of bioactive substances in broiler chickens

Kpodo

Proszkowiec‐Weglarz²

2023

Front. Vet. Sci.

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The poultry industry has improved genetics, nutrition, and management practices, resulting in fast-growing chickens; however, disturbances during embryonic development may affect the entire production cycle and cause irreversible losses to broiler chicken producers. The most crucial time in the chicks' development appears to be the perinatal period, which encompasses the last few days of pre-hatch and the first few days of post-hatch. During this critical period, intestinal development occurs rapidly, and the chicks undergo a metabolic and physiological shift from the utilization of egg nutrients to exogenous feed. However, the nutrient reserve of the egg yolk may not be enough to sustain the late stage of embryonic development and provide energy for the hatching process. In addition, modern hatchery practices cause a delay in access to feed immediately post-hatch, and this can potentially affect the intestinal microbiome, health, development, and growth of the chickens. Development of the in ovo technology allowing for the delivery of bioactive substances into chicken embryos during their development represents a way to accommodate the perinatal period, late embryo development, and post-hatch growth. Many bioactive substances have been delivered through the in ovo technology, including carbohydrates, amino acids, hormones, prebiotics, probiotics and synbiotics, antibodies, immunostimulants, minerals, and microorganisms with a variety of physiological effects. In this review, we focused on the physiological effects of the in ovo delivery of these substances, including their effects on embryo development, gastrointestinal tract function and health, nutrient digestion, immune system development and function, bone development, overall growth performance, muscle development and meat quality, gastrointestinal tract microbiota development, heat stress response, pathogens exclusion, and birds metabolism, as well as transcriptome and proteome. We believe that this method is widely underestimated and underused by the poultry industry.

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“…Supplementation of atRA can lead to inhibition or activation of adipogenesis in the preadipocyte 3T3-L1 model depending on times and concentrations of atRA treatment ( Safonova et al, 1994 ; Suryawan and Hu, 1997 ; Hida et al, 1998 ; Dave et al, 2012 ; Kim et al, 2019 ). Especially, our recent studies using avian species revealed that atRA induced/promoted adipocyte differentiation in vitro ( Kim et al, 2020 ; Lee et al, 2021 ) and increased adipose tissues during the development of chicken embryos ( Kim et al, 2021 ). The understanding of retinoid functions on adipogenesis comes from studies investigating endogenous metabolism of vitamin A.…”

Section: Introductionmentioning

confidence: 99%

Retinol Binding Protein 7 Promotes Adipogenesis in vitro and Regulates Expression of Genes Involved in Retinol Metabolism

Kim

Ahn

Suh

et al. 2022

Front. Cell Dev. Biol.

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Retinol is an essential nutrient in animals. Its metabolites, specifically retinoic acid (RA), are crucial for cell differentiation, including adipogenesis. Retinol binding protein 7 (Rbp7) is under the control of PPARγ, the master regulator of adipogenesis. However, the role of RBP7 in adipogenesis is unclear. Our study showed that Rbp7 was abundantly expressed in white and brown mouse adipose tissues and had a higher expression in adipocytes than in stromal vascular fraction. Rbp7 overexpression promoted 3T3-L1 preadipocyte differentiation with increased triglyceride accumulation and up-regulation of Pparγ, Fabp4, C/ebpα, and AdipoQ. Rbp7 deficient adipocytes had opposite effects of the overexpression, which were rescued by RA supplementation. Indirect assessment of relative nuclear RA levels using RAR response element (RARE)-Luc reporter assay demonstrated that Rbp7 overexpression significantly increased RARE-Luc reporter activity. Rbp7 overexpression significantly increased expression of Raldh1, responsible for RA production, and up-regulation of Lrat and Cyp26a1, involved in retinol storage and RA catabolism, respectively, in 3T3-L1 adipocytes. Rbp7 deficient adipocytes had opposite effects of the overexpression of those genes involved in retinol metabolism. These data suggest that RBP7 increases transcriptional activity of RARE that may induce negative feedback responses via regulation of the gene expression for retinol homeostasis. Our data indicate critical RBP7 functions in adipocytes: regulation of transcriptional activity of RARE and adipocytes differentiation, potentially providing a new target for obesity therapy.

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Hypertrophy of Adipose Tissues in Quail Embryos by in ovo Injection of All-Trans Retinoic Acid

Cited by 6 publications

References 35 publications

Transdifferentiation of Myoblasts Into Adipocytes by All-Trans-Retinoic Acid in Avian

Transdifferentiation of Myoblasts Into Adipocytes by All-Trans-Retinoic Acid in Avian

Physiological effects of in ovo delivery of bioactive substances in broiler chickens

Retinol Binding Protein 7 Promotes Adipogenesis in vitro and Regulates Expression of Genes Involved in Retinol Metabolism

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