Hypertonic saline- and detergent-accelerated EDTA-based decalcification better preserves mRNA of bones
Zhongmin Li,
Clara Wenhart,
Andreas Reimann
et al.
Abstract:Ethylenediaminetetraacetic acid (EDTA), a classically used chelating agent of decalcification, maintains good morphological details, but its slow decalcification limits its wider applications. Many procedures have been reported to accelerate EDTA-based decalcification, involving temperature, concentration, sonication, agitation, vacuum, microwave, or combination. However, these procedures, concentrating on purely tissue-outside physical factors to increase the chemical diffusion, do not enable EDTA to exert it… Show more
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