Hyperthyreosis Effects on the Learning, Memory and Glial Intermediate Filaments of a Rat Brain

Nedzvetskiy, Victor S.; Nerush, P. A.

doi:10.1615/intjphyspathophys.v2.i3.70

Cited by 3 publications

(3 citation statements)

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“…SDS-PAGE was performed in a gradient of polyacrylamide gel (7-18%) in the presence of 0.1% SDS as described in [20]. The content of an intact polypeptide 49 kDa and the composition of GFAP polypeptide fragments were determined by Western blot [21].…”

Section: Methodsmentioning

confidence: 99%

Distribution of glial fibrillary acidic protein in different parts of the rat brain under cadmium exposure

Kovalchuk¹,

Prischepa²,

U³

et al. 2015

Ukr.Biochem.J.

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Section: Methodsmentioning

confidence: 99%

Distribution of glial fibrillary acidic protein in different parts of the rat brain under cadmium exposure

Kovalchuk¹,

Prischepa²,

U³

et al. 2015

Ukr.Biochem.J.

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“…After extraction of insoluble proteins, the samples were centrifuged for 60 min at 60,000 g and a fraction of the water-insoluble proteins was taken. The samples of both soluble and insoluble proteins were separated by electrophoresis in a gradient of a polyacrylamide gel (7-18%) with 0.1% sodium dodecyl sulfate (Nedzvetskiy et al, 2011). The content and polypeptide composition of glial intermediate filaments and S100β were assessed by immunoblotting using a polyclonal monospecific antiserums anti-GFAP (Santa Cruz Biotechnology, USA, cat.…”

Section: Methodsmentioning

confidence: 99%

Molecular mechanisms of aluminium ions neurotoxicity in brain cells of fish from various pelagic areas

Sukharenko

Samoylova

Nedzvetsky

2017

Regul. Mech. Biosyst.

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Neurotoxic effects of aluminum chloride in higher than usual environment concentration (10 mg/L) were studied in brains of fishes from various pelagic areas, especially in sunfish (Lepomis macrochirus Rafinesque, 1819), roach (Rutilus rutilus Linnaeus, 1758), crucian carp (Carasius carasius Linnaeus, 1758), goby (Neogobius fluviatilis Pallas, 1811). The intensity of oxidative stress and the content of both cytoskeleton protein GFAP and cytosol Ca-binding protein S100β were determined. The differences in oxidative stress data were observed in the liver and brain of fish during 45 days of treatment with aluminum chloride. The data indicated that in the modeling of aluminum intoxication in mature adult fishes the level of oxidative stress was noticeably higher in the brain than in the liver. This index was lower by1.5–2.0 times on average in the liver cells than in the brain. The obtained data evidently demonstrate high sensitivity to aluminum ions in neural tissue cells of fish from various pelagic areas. Chronic intoxication with aluminum ions induced intense astrogliosis in the fish brain. Astrogliosis was determined as result of overexpression of both cytoskeleton and cytosole markers of astrocytes – GFAP and protein S100β (on 75–112% and 67–105% accordingly). Moreover, it was shown that the neurotixic effect of aluminum ions is closely related to metabolism of astroglial intermediate filaments. The results of western blotting showed a considerable increase in the content of the lysis protein products of GFAP with a range of molecular weight from 40–49 kDa. A similar metabolic disturbance was determined for the upregulation protein S100β expression and particularly in the increase in the content of polypeptide fragments of this protein with molecular weight 24–37 kDa. Thus, the obtained results allow one to presume that aluminum ions activate in the fish brain intracellular proteases which have a capacity to destroy the proteins of intermediate filaments. The data presented display the pronounced neurotoxic effect of mobile forms of aluminum on both expression level and the metabolism of molecular markers of astrocytes GFAP and protein S100β. Aluminum ions induce integrated changes, the more important of which are a significant increase in final LPO products, an increase in antioxidant enzyme activity, a reactivation of glial cells in the brain. Integrated determination of the content and polypeptide fragments of specific astrocyte proteins in fishes brains coupled with oxidative stress data may be used as valid biomarkers of toxic pollutant effects in aquatic environments.

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“…Несприятливі дії різної природи індукують характерну клітинну відповідь гліїастрогліоз. Астрогліоз, тобто реактивация астроцитів, завжди супроводжується активацією фібрилогенезу та синтезу ГФКБ (Nedzvetskiy and Nerush, 2011). Надмірно інтенсивний фібрилогенез є головним показником реактивної відповіді астроцитів на нейрональні ушкодження.…”

Section: рис 2 результати імуноблотингу цитоскелетних фракцій білкіunclassified

Використання Специфічних Біомаркерів Донних Риб Керченської Протоки Для Оцінки Забруднення Водних Біоресурсів

Sukharenko¹

2014

VDNUBM

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The modern ecology situation in waters of the Kerch Strait requires assessment of disturbances in biotopes and monitoring of the degree of impact of industrial pollutants on ecosystem. Deposit of oil products after the 2007 year ships’ accidents might have considerable impact on the water biocenosis area. The investigation of cytoskeleton marker of astrocytes glial fibrillary acidic protein (GFAP) in brain of the bullhead (Neogobius fluviatilis), which is the typical representative of the commercial ground fish of the Kerch Strait, has been carried out. The results of comparative analysis of GFAP content in the brain of fish from the Kerch Bay near-shore waters and fish from conditionally clear area of Vorskla river shows the reliable (2.18 times) increasing of GFAP in the area of industrial pollution. Rising GFAP content indicates the astrogliosis development as a result of metabolic disturbances which can be induced by higher content of oil products in the near-bottom biotopes of the Kerch Bay. Increase in lipid peroxidation level was observed in the brain of fish from the Kerch Bay. The results provided with regard to violations of the state of astrocyte cytoskeleton and oxidative stress in the brain of bullhead from the Kerch Bay prove the sublethal biology effect of industrial pollutants in hydrobionts from this area. Results of this investigation also indicate the necessity of continuous ecology monitoring and comprehensive study of hydrobiont populations in the industrial regions and ecological disaster zones.

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Hyperthyreosis Effects on the Learning, Memory and Glial Intermediate Filaments of a Rat Brain

Cited by 3 publications

References 0 publications

Distribution of glial fibrillary acidic protein in different parts of the rat brain under cadmium exposure

Distribution of glial fibrillary acidic protein in different parts of the rat brain under cadmium exposure

Molecular mechanisms of aluminium ions neurotoxicity in brain cells of fish from various pelagic areas

Використання Специфічних Біомаркерів Донних Риб Керченської Протоки Для Оцінки Забруднення Водних Біоресурсів

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