Hyperthermic Preconditioning of Presynaptic Calcium Regulation in<i>Drosophila</i>

Klose, Markus K.; Atwood, Harold L.; Robertson, R. Meldrum

doi:10.1152/jn.01251.2007

Cited by 26 publications

(37 citation statements)

References 52 publications

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“…In addition to the N-type calcium channel, Hsp70s interact with the cytosolic loop of Ca V 2.3 R-type voltage-gated calcium channel [89]. The interaction may be important in decreasing the intracellular calcium overload that accompanies hyperthermic insults [25]. Prior to heat shock or induced elevation, Hsp70 at the D. melanogaster neuromuscular junction stabilizes the intracellular calcium concentration by maintaining near-resting calcium level and promoting calcium clearance in subsequent hyperthermic episodes.…”

Section: Hsp70 In the Stressed Synapsementioning

confidence: 99%

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Multifaceted Role of Heat Shock Protein 70 in Neurons

Quan

Feng

2010

Mol Neurobiol

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Section: Hsp70 In the Stressed Synapsementioning

confidence: 99%

“…Increasing Hsp expression level by overexpression [24], drug induction [21], genetic manipulation [25] and preconditioning [20,[26][27][28] protects the cells/organisms from lethal conditions. The mechanisms underlying the cytoprotective effects of heat shock proteins, specifically the Hsp70s, have been well delineated.…”

Section: Introductionmentioning

confidence: 99%

Multifaceted Role of Heat Shock Protein 70 in Neurons

Quan

Feng

2010

Mol Neurobiol

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“…Synaptic failure at the Drosophila neuromuscular junction results from a loss of the ability to regulate intracellular Ca 2ϩ concentrations (Klose et al, 2008). Given these precedents and the time dependence of recovery of the tadpole swim CPG, we believe it to be a reasonable supposition that hyperthermic failure and recovery in this system are closely related to loss and restoration of appropriate ion gradients.…”

Section: Discussionmentioning

confidence: 93%

“…Long-term processes of acclimation (Johnston and Temple, 2002) can modify neuronal properties and change optimal temperature ranges (Behan-Martin et al, 1993). More rapid processes requiring protein synthesis can be activated by heat shock pretreatments (Robertson et al, 1996;Kelty et al, 2002;Armstrong et al, 2006;Rodgers et al, 2007), and in larval Drosophila the stress protein HSP70 has been shown to have a role in protecting locomotion (Xiao et al, 2007), and calcium dynamics at the neuromuscular junction (Klose et al, 2008), from hyperthermia. Recently, the nitric oxide (NO)/ cGMP/protein kinase G pathway has been implicated in rapid adaptation of the temperature sensitivity of motor circuits in insects (Dawson-Scully et al, 2007), but it is not known whether similar mechanisms are operational in vertebrates.…”

Section: Introductionmentioning

confidence: 99%

The Nitric Oxide/cGMP Pathway Tunes the Thermosensitivity of Swimming Motor Patterns inXenopus laevisTadpoles

Robertson

Sillar²

2009

J. Neurosci.

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We investigated the role of the nitric oxide (NO)/cGMP pathway in setting thresholds for failure and recovery during hyperthermic stress of the swimming central pattern generator of immobilized Xenopus tadpoles (stage 42). We recorded swimming motor patterns induced by tail skin stimulation (TS) (1 ms current pulse) or by bath application of 50 M NMDA. Swimming rhythm frequency increased in a linear manner with increasing temperature. In the presence of the NO donor S-nitroso-N-acetylpenicillamine (SNAP), recovery from hyperthermic failure was greatly slowed, often taking longer than the duration of the experiment. Pharmacological activation of the NO/cGMP pathway using SNAP or 8-bromo-cGMP (1) decreased the duration of TS-evoked swim episodes; (2) decreased the temperature threshold for hyperthermic circuit failure; (3) decreased the temperature at which the circuit recovered; and (4) increased the time taken to recover. Pharmacological inhibition of the NO/cGMP pathway using the NO scavenger CPTIO, the nitric oxide synthase (NOS) inhibitor L-NAME or the guanylyl cyclase inhibitor ODQ (1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one) had the opposite effects. NMDA rhythms were more resistant to hyperthermic failure than TS-evoked swim episodes, but the effects of SNAP on the temperature sensitivity of swimming evoked by NMDA were similar to those on TS-evoked swimming, suggesting that drug effects occur on central patterngenerating networks rather than sensory pathways. We conclude that the NO/cGMP pathway is involved in setting the threshold temperatures for hyperthermic failure and subsequent recovery of fictive swimming in tadpoles, and we suggest that this is part of a variable response to prevent overexcitation during abiotic stress under different environmental conditions.

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“…Gangguan regulasi Ca 2+ yang penting untuk presynaps menyebabkan kejadian synaptic plasticity selama beberapa menit sehingga menurunkan aliran impuls synaps. 20 Impuls yang terputus ini menyebabkan ekspresi TNF-α dan SP, biomarker yang terlibat dalam neurogenic switching juga menurun dengan cepat.…”

Section: Analisis Varians (Anova) Pembahasanunclassified

Menurunkan Biomarker Migren Secara Cepat dengan Terapi “Assisted Drainage” (Studi Eksperimental pada Hewan coba)

Utomo

2015

Majalah Kedokteran Gigi Indonesia

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ABSTRAKMigren merupakan gejala nyeri kepala rekuren yang paling sering dikeluhkan dalam dunia medis. Di Amerika Serikat, lebih dari 30 juta orang menderita satu atau lebih nyeri kepala migren dalam setahun. Berdasarkan literatur, migren merupakan komorbiditas rinosinusitis dan asma serta melibatkan saraf trigeminus. Tatalaksana migren dalam bidang kedokteran adalah obat-obatan dan tindakan invasif seperti injeksi atau operasi pada saraf. Sangat menarik bahwa menurut laporan kasus telah dibuktikan bahwa migren berhubungan dengan gingivitis karena suatu perawatan periodontal non invasif yang disebut sebagai terapi "assisted drainage" (ADT) dapat mengurangi gejala migren dalam hitungan menit. Terapi tersebut berupa masase sulkus gingiva dalam 3 menit dengan sisi tumpul scaler manual sampai timbul keluarnya darah secara pasif. Akan tetapi mekanisme kejadian pengurangan gejala migren setelah ADT masih belum jelas. Tujuan penelitian adalah untuk verifikasi pengaruh ADT terhadap penurunan biomarker migren tikus nonalergi dan alergi yang gingivitis. Tikus Wistar dibagi menjadi dua kelompok secara acak, penelitian dengan control series design study. Kelompok perlakuan adalah tikus alergi yang diinduksi dengan ovalbumin (OVA) dan non-alergi, kelompok ini disuntik dengan lipopolisakarida Porphyromonas gingivalis. Kelompok kontrol positif adalah tikus alergi, kontrol negatif dengan phosphate buffered saline (PBS). Pada beberapa kelompok dilakukan ADT. Ekspresi substans P (SP), leukotrien C4 (LTC4) dan TNF α pada gingiva dan hidung diperiksa dengan imunohistokimia peroksidase. PENDAHULUANMigren adalah nyeri kepala neurovaskuler kambuhan yang ditandai dengan nyeri kepala berdenyut parah berkala, disertai mual, muntah, fotofobia, fonofobia, enggan melakukan aktivitas fisik. Prevalensi migren meningkat dari 4% sebe lum pubertas sampai pada puncaknya, yaitu 25% pada wanita usia subur serta berkurang setelah menopause. Kurang lebih 30 juta penduduk Amerika Serikat dewasa (18% wanita dan 6% of pria) menderita migren. ARTIKEL PENELITIAN

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Hyperthermic Preconditioning of Presynaptic Calcium Regulation inDrosophila

Abstract: Klose MK, Atwood HL, Robertson RM. Hyperthermic preconditioning of presynaptic calcium regulation in Drosophila. J Neurophysiol

Cited by 26 publications

References 52 publications

Multifaceted Role of Heat Shock Protein 70 in Neurons

Multifaceted Role of Heat Shock Protein 70 in Neurons

The Nitric Oxide/cGMP Pathway Tunes the Thermosensitivity of Swimming Motor Patterns inXenopus laevisTadpoles

Menurunkan Biomarker Migren Secara Cepat dengan Terapi “Assisted Drainage” (Studi Eksperimental pada Hewan coba)

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