Hyperthermia Severely Affects the Vascular Effects of MDMA and Metabolites in the Human Internal Mammary Artery In Vitro

Fonseca, Diogo A.; Guerra, A. F.; Carvalho, Félix; Fernandes, Eduarda; Ferreira, Laura Fernandes; Branco, Paula S.; Antunes, Pedro E.; Antunes, Manuel J.; Cotrim, Maria Dulce

doi:10.1007/s12012-017-9398-y

Cited by 5 publications

(2 citation statements)

References 41 publications

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“…Vessel samples from 54 patients were harvested and prepared as described previously [13]. Briefly, ITAs were harvested as a pedicle after sternal incision and were externally irrigated with papaverine to prevent vasospasm.…”

Section: Methodsmentioning

confidence: 99%

Histomorphometric analysis of the human internal thoracic artery and relationship with cardiovascular risk factors

Fonseca

Antunes

et al. 2019

PLoS ONE

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In this study, we aimed at performing a histomorphometric analysis of human left internal thoracic artery (ITA) samples as well as at correlating the histomorphometric findings with the clinical profile, including risk factors and medication. Distal segments of ITA were obtained from 54 patients undergoing coronary artery bypass grafting. Histological observation was performed in paraffin-embedded transverse sections of ITA through four staining protocols: hematoxylin-eosin, van Gieson, Masson’s trichrome and von Kossa. Morphometric analysis included the intimal width (IW), medial width (MW) and intima/media ratio (IMR). No overt atherosclerotic lesions were observed. Mild calcifications were observed across the vascular wall layers in almost all samples. Multivariable linear regression analysis showed associations between IW and IMR and the following clinical variables: age, gender, kidney function expressed as eGFR and myocardial infarction history. Age (odds ratio = 1.16, P = 0.004), female gender (odds ratio = 11.34, P = 0.011), eGFR (odds ratio = 1.03, P = 0.059) and myocardial infarction history (odds ratio = 4.81, P = 0.040) were identified as the main clinical predictors for intimal hyperplasia. Preatherosclerotic lesions in ITA samples from patients undergoing coronary revascularization were associated not only with classical cardiovascular risk factors such as age and gender, but also with other clinical variables, namely kidney function and myocardial infarction history.

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Section: Methodsmentioning

confidence: 99%

Histomorphometric analysis of the human internal thoracic artery and relationship with cardiovascular risk factors

Fonseca

Antunes

et al. 2019

PLoS ONE

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“…Vessel samples from 79 patients were harvested and prepared as described previously (Fonseca et al., ). Briefly, ITAs were harvested as a pedicle after sternal incision and were externally irrigated with papaverine to prevent vasospasm.…”

Section: Methodsmentioning

confidence: 99%

Vasomotion as an oscillatory sign of functional impairment in the human internal thoracic artery: A study based on risk factors and vessel reactivity

Fonseca

Antunes

et al. 2018

Experimental Physiology

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Vasomotion has been defined as the rhythmic oscillation of the vascular tone, involved in the control of the blood flow and subsequent tissue perfusion. Our aims were to study the incidence of vasomotion in the human internal thoracic artery and the correlation of this phenomenon with the clinical profile and parameters of vascular reactivity. In our study, vasomotion was elicited with a single-dose contractile stimulation of noradrenaline (10 μm) in internal thoracic artery segments, from patients undergoing coronary artery bypass grafting, mounted in tissue organ bath chambers. The incidence was 29.1%. Vessel samples with vasomotion presented significantly higher contractility in response to both potassium chloride (maximal response or E of 7.65 ± 5.81 mN versus 4.52 ± 3.73 mN in control vessels, P = 0.024) and noradrenaline (E of 7.60 ± 5.93 mN versus 2.96 ± 4.41 mN in control vessels, P < 0.001). Predictive modelling through multivariable logistic regression analysis showed that female sex (odds ratio = 9.82) and increasing maximal response to noradrenaline (odds ratio = 1.19, per 1 mN increase) were associated with a higher probability of the occurrence of vasomotion, whereas increasing kidney function (expressed as estimated glomerular filtration rate) was associated with a lower probability (odds ratio = 0.97, per 1 ml min (1.73 m) ]. Our results provide a characterization of the phenomenon of vasomotion in the internal thoracic artery and suggest that vasomotion might be associated with endothelial dysfunction settings, as determined by a multivariable analysis approach. Considering the associations observed in our results, vasomotion might be a signal of functional impairment and not of integrity.

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Ecstasy (3,4-methylenedioxymethamphetamine): Cardiovascular effects and mechanisms

Fonseca

Ribeiro

Tapadas

et al. 2021

European Journal of Pharmacology

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Hyperthermia Severely Affects the Vascular Effects of MDMA and Metabolites in the Human Internal Mammary Artery In Vitro

Cited by 5 publications

References 41 publications

Histomorphometric analysis of the human internal thoracic artery and relationship with cardiovascular risk factors

Histomorphometric analysis of the human internal thoracic artery and relationship with cardiovascular risk factors

Vasomotion as an oscillatory sign of functional impairment in the human internal thoracic artery: A study based on risk factors and vessel reactivity

Ecstasy (3,4-methylenedioxymethamphetamine): Cardiovascular effects and mechanisms

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