Hyperthermia-induced protein corona improves the therapeutic effects of zinc ferrite spinel-graphene sheets against cancer

Abstract

Superparamagnetic zinc ferrite spinel-graphene nanostructures were synthesized as potential therapeutic agents in the magnetic targeted photothermal therapy of cancer and/or drug delivery. The global temperature of the solution and the local temperature at the nanoparticle (NP) surface determine the protein corona composition/content, which in turn affects the biological effects of NPs and the corresponding physiological responses. Therefore, it is rational to hypothesize that spinel-graphene nanostructures may have distinct protein corona compositions and contents, and therapeutic and toxic effects under laser irradiation. To assess this hypothesis, the effects of laser irradiation on the competitive binding of proteins to the spinel-graphene surface and the corresponding therapeutic effects of corona-coated spinel-graphene nanomaterials against cancer were evaluated. The results showed that the composition and content of the protein corona adsorbed onto the spinel-graphene nanomaterials was correlated with the local and global heating caused by laser irradiation. Moreover, the corona-coated spinel-graphene nanomaterials obtained following laser irradiation induced a higher intracellular concentration of reactive oxygen species (ROS) and exhibited a significantly higher cellular uptake efficacy compared to that achieved in the absence of laser irradiation (37 C). This finding indicates that the therapeutic effects of graphene-based photothermal therapy against cancer are, in part, related to the composition and content of the protein corona formed under laser irradiation. The results of this study reveal that changes in the composition and content of the protein corona that occur during laser irradiation affect the therapeutic and toxic effects of NPs.

Superparamagnetic Gadolinium Ferrite Nanoparticles with Controllable Curie Temperature – Cancer Theranostics for MR‐Imaging‐Guided Magneto‐Chemotherapy

“…Our studies suggest that hyperthermia combined with DOX‐loaded GdIO NPs can significantly enhance ROS generation and thus increase the cancer cell killing rate. Similar behavior has also been observed in recent reports …”

“…Similar behavior has also been observed in recent reports. [41] In addition to ROS measurements, cellular apoptosis was evaluated with fluorescence activated cell sorting (FACS), flow cytometry analysis, and confocal microscopy by using propid- ium iodide (PI), 4′,6-diamidino-2-phenylindole (DAPI), and fluorescein isothiocyanate (FITC) as stains. It is well-known that a nucleus dye (i.e.…”

Analysis of the Human Plasma Proteome Using Multi‐Nanoparticle Protein Corona for Detection of Alzheimer's Disease

“…[17] Indeed, the specific content of the PC dramatically affects the biological fate of the NPs in terms of uptake and interactions with cells, pharmacokinetics, therapeutic efficacy, toxicity, and circulation time. [20,[22][23][24][25][26][27][28][29][30][31] It is increasingly being accepted that alterations in the plasma proteome, caused by a spectrum of diseases, can affect the PC composition, generating disease-specific protein coronas. [32][33][34] Therefore, characterization of personalized PCs may become a tool for the detection of variations in plasma protein concentrations and a valuable alternative method for capturing fluctuations in circulating protein concentrations undetectable with conventional blood analysis.…”

Molecular interaction of fibrinogen with zeolite nanoparticles

“…In fact, the formation of protein corona changes the surface properties of NPs and it provides a biological mask, which is being “seen” by the biological systems such as cells 7,18–21 . The type, concentration and configuration of the participated proteins in the corona layer can give useful information for predicting the biological fate of NPs including their pharmacokinetics and biodistribution 10,14,22–25 .…”